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A human/mouse chimeric monoclonal antibody against CA125 for radioimmunoimaging of ovarian cancer

  • Original Articles
  • Monoclonal Antibody, Immunodetection, CA125, Ovarian Cancer
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

Murine monoclonal antibody 196-14 recognizes the ovarian-cancer-associated antigen CA 125, but the epitope it recognizes is different from that of monoclonal antibody OC125. We developed a human/mouse chimeric 196-14 using the variable regions of the murine 196-14 and human heavy-chain (γl) and light-chain (κ) constant regions. Cell binding and competitive inhibition assays using chimeric 196-14 labeled with125I,111In or99mTc demonstrated that the in vitro immunoreactivity of the chimeric antibody was identical to that of the parental murine monoclonal antibody. However, in mice bearing human ovarian cancer xenografts, the clearance from blood was faster and absolute levels of accumulation in the tumor were lower for the125I-labeled or99mTc-labeled chimeric antibody than for the murine antibody labeled with the corresponding radionuclides. The tumor-to-blood radioactivity ratio was not significantly different between the chimeric antibody and the murine antibody, regardless of the radionuclide used for labeling. Chimeric antibody 196-14 labeled with131I,111In or99mTc is promising for the radioimmunoimaging of ovarian cancer.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Kyoto University Faculty of Medicine, 54, Kawahara-cho, Shogoin, Sakyo-ku, 606, Kyoto, Japan

    Hisataka Kobavashi, Harumi Sakahara, Tsuneo Saga, Makoto Hosono, Makoto Shirato & Junji Konishi

  2. Eiken Chemical Co. Ltd., Tokyo, Japan

    Hidetoshi Kanda & Kaichiro Ishibashi

  3. Department of Molecular Immunology, Medical Institute of Bioregulation, Kyusyu University, Fukuoka, Japan

    Takeshi Watanabe

  4. Department of Nuclear Medicine, Gunma University School of Medicine, Gunma, Japan

    Keigo Endo

  5. Ishiwata Obstetrics and Gynecology Hospital, Mito, Ibaragi, Japan

    Isamu Ishiwata

Authors
  1. Hisataka Kobavashi
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  2. Harumi Sakahara
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  3. Tsuneo Saga
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  4. Makoto Hosono
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  5. Makoto Shirato
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  6. Hidetoshi Kanda
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  7. Kaichiro Ishibashi
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  8. Takeshi Watanabe
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  9. Keigo Endo
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  10. Isamu Ishiwata
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  11. Junji Konishi
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Kobavashi, H., Sakahara, H., Saga, T. et al. A human/mouse chimeric monoclonal antibody against CA125 for radioimmunoimaging of ovarian cancer. Cancer Immunol Immunother 37, 143–149 (1993). https://doi.org/10.1007/BF01525427

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  • DOI: https://doi.org/10.1007/BF01525427

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