Abstract
We raised an anti-glioma monoclonal antibody, named G-22, that specifically recognizes a human gliomaassociated surface antigen. Proven to be useful for target imaging of malignant gliomas after radioisotope labeling and cerebrospinal fluid diagnosis by enzyme-linked immunospecific assay, G-22 was found to immunoprecipitate an 83-kDa glycoprotein of the human glioma U-251MG cell. We purified this antigen by G-22-coupled cyanogenbromide-activated Sepharose affinity chromatography, and sequence analysis demonstrated that the 54 amino acid residues were identical to positions 55–108 of human CD44. The results show that the smallest spliced form (85 kDa) of CD44 is strongly expressed in glioma cells.
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Okada, H., Yoshida, J., Seo, H. et al. Anti-(glioma surface antigen) monoclonal antibody G-22 recognizes overexpressed CD44 in glioma cells. Cancer Immunol Immunother 39, 313–317 (1994). https://doi.org/10.1007/BF01519984
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DOI: https://doi.org/10.1007/BF01519984