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Long-term serum/plasma-free culture of human cytotoxic T lymphocytes induced from peripheral blood mononuclear cells

  • Original Articles
  • Cytotoxic T Lymphocyte, Serum-free Culture, Interleukin, Killing Activity Adoptive Immunotherapy
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Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

Tumor-specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin(IL)-1, IL-2, IL-4 and IL-6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free medium containing these four interleukins and 0.5% bovine serum albumin for over a month and maintained kiling activity of target cells within 48 h at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. Under these conditions, IL-4 and IL-6 could be omitted. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and IL-2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (1011) were obtained in 35 days.

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Authors and Affiliations

  1. RIKEN Cell Bank, The Institute of Physical and Chemical Research (RIKEN), Koyadai 3-1-1, 305, Tsukuba Science City, Ibaraki, Japan

    Shu Qin Liu, Reiko Shiba, Berm Soo Kim, Kaoru Saijo & Tadao Ohno

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  1. Shu Qin Liu
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  2. Reiko Shiba
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  3. Berm Soo Kim
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  4. Kaoru Saijo
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  5. Tadao Ohno
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Liu, S.Q., Shiba, R., Kim, B.S. et al. Long-term serum/plasma-free culture of human cytotoxic T lymphocytes induced from peripheral blood mononuclear cells. Cancer Immunol Immunother 39, 279–285 (1994). https://doi.org/10.1007/BF01519979

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  • DOI: https://doi.org/10.1007/BF01519979

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