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Scintigraphic detection of xenografted tumors producing human basic fibroblast growth factor

  • Original Articles
  • Basic Fibroblast Growth Factor, Monoclonal Antibody, Scintigraphy
  • Published:
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Abstract

A murine monoclonal antibody 3H3 recognizes the basic fibroblast growth factor (FGF) and inhibits the growth of human glioblastoma cells both in vitro and in vivo. We studied the potential of a scintigraphic technique using the 3H3 antibody to detect tumors that produce basic FGF.125I- and111In-labeled 3H3 bound to U87MG human glioblastoma cells in vitro. U87MG cells were inoculated subcutaneously into nude mice. After development of the tumor, radiolabeled 3H3 was injected into the subcutaneous space surrounding the tumor. A high level of radioactivity from 3H3 was retained at the tumor, whereas an irrelevant antibody cleared rapidly from the injected site. Radiolabeled 3H3 was not retained in tumors that did not produce basic FGF. Scintigraphic detection of tumors expressing basic FGF would be valuable for the therapeutic application of the antibody.

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References

  1. Abdel-Nabi HH, Schwartz AN, Higano CS, Wechter DG, Unger MW (1987) Colorectal carcinoma: detection with indium-111 anticarcinoembryonic-antigen monoclonal antibody ZCE-025. Radiology 164:617

    PubMed  Google Scholar 

  2. Carrasquillo JA, Mulshine JL, Bunn PA Jr, Reynolds JC, Foon KA, Schroff RW, Perentesis P, Steis RG, Keenan AM, Horowitz M, Larson SM (1987) Indium-111-T101 monoclonal antibody is superior to iodine-131-T101 in imaging of cutaneous T-cell lymphoma. J Nucl Med 28: 281

    PubMed  Google Scholar 

  3. Chatal JF, Saccavini JC, Gestin JF, Thedrez P, Curtet C, Kremer M, Guerreau D, Nelibe D, Fumoleau P, Guillard Y (1989) Biodistribution of indium-111-labeled OC125 monoclonal antibody intraperitoneally injected into patients operated on for ovarian carcinomas. Cancer Res 49: 3087

    PubMed  Google Scholar 

  4. Folkman J, Klagsbrun M (1987) Angiogenic factors. Science 235: 442

    PubMed  Google Scholar 

  5. Gospodarowicz D, Neufeld G, Schweigerer L, (1987) Fibroblast growth factor: structural and biological properties. J Cell Physiol [Suppl] 5: 15

    Google Scholar 

  6. Gospodarowicz D, Ferrara N, Schweigerer L, Neufeld G (1987) Structural characterization and biological functions of fibroblast growth factor. Endocrinol Rev 8: 95

    Google Scholar 

  7. Greenwood FC, Hunter WN, Glover JS (1963) The preparation of131I labeled human growth hormone of high specific radioactivity. Biochem J 89: 114

    PubMed  Google Scholar 

  8. Hunter WN, Greenwood FC (1962) Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature 194: 495

    PubMed  Google Scholar 

  9. Kalofonos HP, Pawlikowska TR, Hemingway N, Courtenay-Luck N, Dhokia B, Snook D, Sivolapenko GB, Hooker GR, McKenzie CG, Lavender PJ, Thomas DGT, Epenetos AA (1989) Antibody guided diagnosis and therapy of brain gliomas using radiolabeled monoclonal antibodies against epidermal growth factor receptor and placental alkaline phosphatase. J Nucl Med 30: 1636

    PubMed  Google Scholar 

  10. Kurokawa T, Sasada R, Iwane M, Igarashi K (1987) Cloning and expression of cDNA encoding human basic fibroblast growth factor. FEBS Lett 213: 189

    PubMed  Google Scholar 

  11. Libermann TA, Nusbaum HR, Razon N, Kris R, Lax I, Soreq H, Whittle N, Waterfield MD, Ullrich A, Schlessinger J (1985) Amplification, enhanced expression, and possible rearrangement of EGF receptor gene in primary human brain tumors of glial origin. Nature (Lond) 313: 144

    Google Scholar 

  12. Libermann TA, Friesel R, Jaye M, Lyall RM, Westmark B, Drohan W, Schmidt A, Maciag T, Schlessinger J (1987) An angiogenic growth factor is in human glioma cells. EMBO J 6: 1627

    PubMed  Google Scholar 

  13. Lindmo T, Boven E, Cuttitta F, Fedorko J, Bunn PA, Jr (1984) Determination of immunoreactive fraction of radiolabeled monoclonal antibodies by linear extrapolation to binding at infinite antigen excess. J Immunol Methods 72: 77

    PubMed  Google Scholar 

  14. Macauly VM, Teale JD, Everard MJ, Joshi GP, Smith IE, Millar JL (1988) Somatomedin-C/insulin-like growth factor-I is a mitogen for human small cell lung cancer. Br J Cancer 57: 91

    PubMed  Google Scholar 

  15. Muller WJ, Sinn E, Pattengale PK, Wallace R, Leder P (1988) Single-step induction of mammary adenocarcinoma in transgenic mice bearing the activated c-neu oncogene. Cell 54: 105

    PubMed  Google Scholar 

  16. Murray JL, Rosenblum MG, Sobol RE, Bartholomew RM, Plager CE, Haynie TP, Jahns MF, Glenn HJ, Lamki L, Benjamin RS, Papadopoulos N, Boddie AW, Frincke JM, David GS, Carlo DJ, Hersh EM (1985) Radioimmunoimaging in malignant melanoma with111In-labeled monoclonal antibody 96.5. Cancer Res 45: 2376

    PubMed  Google Scholar 

  17. Ponten J, Macintyre EH (1968) Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand 74: 465

    PubMed  Google Scholar 

  18. Saga T, Ishiwata I, Endo K, Sakahara H, Koizumi M, Watanabe Y, Nakai T, Hosono M, Ishikawa H, Sawada M, Konishi J (1990) An antibody-tumor model for the targeting of CA 125-producing gynecologic alignancies. Jpn J Cancer Res 81: 1141

    PubMed  Google Scholar 

  19. Saga T, Endo K, Akiyama T, Sakahara H, Koizumi M, Watanabe Y, Nakai T, Hosono M, Yamamoto T, Toyoshima K, Konishi J (1991) Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody. Cancer Res 51: 990

    PubMed  Google Scholar 

  20. Sakahara H, Endo K, Nakashima T, Koizumi M, Ohta H, Torizuka K, Furukawa T, Ohmoto Y, Yokoyama A, Okada K, Yoshida O, Nishi S (1985) Effect of DTPA conjugation on the binding activity and biodistribution of monoclonal antibodies against alpha-fetoprotein. J Nucl Med 26: 750

    PubMed  Google Scholar 

  21. Sakahara H, Endo K, Nakashima T, Koizumi M, Kunimatsu M, Kawamura Y, Ohta H, Nakamura T, Tanaka H, Kotoura Y, Yamamuro T, Hosoi S, Toyama S, Torizuka K (1987), Localization of human osteogenic sarcoma xenografts in nude mice by a monoclonal antibody labeled with radioiodine and indium-111. J Nucl Med 28: 342

    PubMed  Google Scholar 

  22. Sato Y, Murphy PR, Sato R, Friesen HG (1989) Fibroblast growth factor release by bovine endothelial cells and human astrocytoma cells in culture is density dependent. Mol Endocrinol 3: 744

    PubMed  Google Scholar 

  23. Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51: 660

    Google Scholar 

  24. Seno K, Sasada R, Iwane K, Iwane M, Sudo K, Kuroiwa T, Ito K, Igarashi K (1988) Stabilizing basic fibroblast growth factor using protein engineering. Biochem Biophys Res Commun 151: 701

    PubMed  Google Scholar 

  25. Sporn MB, Roberts AB (1985) Autocrine growth factors and cancer. Nature 313: 745

    PubMed  Google Scholar 

  26. Takahashi JA, Mori H, Fukumoto M, Igarashi K, Jaye M, Oda Y, Kikuchi H, Hatanaka M (1990) Gene expression of fibroblast growth factors in human gliomas and meningiomas: demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues. Proc Natl Acad Sci 87: 5710

    PubMed  Google Scholar 

  27. Takahashi JA, Fukumoto M, Kozai Y, Ito N, Oda Y, Kikuchi H, Hatanaka M (1991) Inhibition of cell growth and tumorgenesis of human glioblastoma cells by a neutralizing antibody against human basic fibroblast growth factor. FEBS Lett 288: 65

    PubMed  Google Scholar 

  28. Takahashi JA, Suzui H, Yasuda Y, Ito N, Ohta M, Jaye M, Fukumoto M, Oda Y, Kikuchi H, Hatanaka M (1991) Gene expression of fibroblast growth factor receptors in the tissues of human gliomas and meningiomas. Biochem Biophys Res Commun 177: 1

    PubMed  Google Scholar 

  29. Takahashi JA, Fukumoto M, Igarashi K, Oda Y, Kikuchi H, Hatanaka M (1992) Correlation of basic fibroblast growth factor expression levels with the degree of malignancy and vascularity in human gliomas. J Neurosurg 76: 792

    PubMed  Google Scholar 

  30. Tom BH, Rutzky LP, Jakstys MM (1976) Human colonic adenocarcinoma cell. I. Establishment and description of a new line. In Vitro (Rockville) 12: 180

    Google Scholar 

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Authors and Affiliations

  1. Department of Nuclear Medicine, Faculty of Medicine, Kyoto University, Shogoin, Sakyo-ku, Kyoto, Japan

    Hisataka Kobayashi, Harumi Sakahara, Makoto Hosono, Makoto Shirato & Junji Konishi

  2. Department of Neurosurgery, Faculty of Medicine, Kyoto University, Kyoto, Japan

    Jun A Takahashi, Yoshifumi Oda & Haruhiko Kikuchi

  3. Department of Nuclear Medicine, School of Medicine, Gunma University, Gunma, Japan

    Keigo Endo

  4. Biology Research Laboratories, Takeda Chemical Industries Ltd., Osaka, Japan

    Yoshio Kozai

  5. Department of Molecular Virology, Institute for Virus Research, Kyoto University, Kyoto, Japan

    Masakazu Hatanaka

Authors
  1. Hisataka Kobayashi
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  2. Harumi Sakahara
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  3. Makoto Hosono
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  4. Makoto Shirato
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  5. Junji Konishi
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  6. Jun A Takahashi
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  7. Yoshifumi Oda
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  8. Haruhiko Kikuchi
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  9. Keigo Endo
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  10. Yoshio Kozai
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  11. Masakazu Hatanaka
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Kobayashi, H., Sakahara, H., Hosono, M. et al. Scintigraphic detection of xenografted tumors producing human basic fibroblast growth factor. Cancer Immunol Immunother 37, 281–285 (1993). https://doi.org/10.1007/BF01518449

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  • DOI: https://doi.org/10.1007/BF01518449

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