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Immunological characterization of tumor-rejection antigens on ultraviolet-light-induced tumors originating in the CB6F1 mouse

  • Original Articles
  • UV-Induced Tumor, Tumor Rejection Antigen Ultraviolet Light
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Abstract

Six ultraviolet-light(UV)-induced tumors of (BALB/c×C57BL/6)F1 (H-2d/b) mouse origin were analyzed for the effector T cell subsets involved in tumor rejection the MHC class I to which cytolytic T lymphocytes (CTL) are restricted, and the effect of UV radiation on tumor rejection, to characterize their tumor-rejection antigens (TRA) recognized by CTL. All tumors were rejected in syngeneic normal mice but grew progressively in nude mice. CD8+ T cells mediated the antitumor responses for all tumors and CD4+ T cells could also do so for one tumor 6.1B. Each tumor induced potent CTL that recognized the specific TRA in preferential association with MHC class I haplotypes not from H-2b but from H-2d; that is, Kd, Dd or Ld. Profiles of TRA expression on two tumors were obtained by the analyses of their antigen-loss variants. ♀1A codominantly expressed at least four distinct TRA associated with Kd, all of which induced CTL. On the other hand, UV♂ 1 had at least two distinct TRA, one of which, associated with Kd, exclusively induced CTL. However, in the absence of the dominant TRA, another TRA associated with Ld on R95C, a variant of UV♂, 1, induced CTL. Unlike other tumors, R95C grew progressively in short-term-UV-irradiated syngeneic mice. Nude mice reconstituted with a combination of CD4+ T cells from short-term-UV-irradiated mice and CD8+ T cells from normal mice did not reject R95C. An increase in the former T cell population led the reconstituted mice to reject the tumor. These findings suggest some functional defects of CD4+ T cells rather than the generation of suppressor cells in short-term-UV-irradiated mice. The UV-induced tumors used in the present study provide a unique system for analyzing the preferential sorting of TRA as well as for elucidation of the TRA itself.

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Authors and Affiliations

  1. Department of Dermatology, Faculty of Medicine, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    Toshiyuki Kitajima & Sadao Imamura

  2. Institute for Immunology, Faculty of Medicine, Kyoto University, Kyoto, Japan

    Michihiro Iwashiro & Kagemasa Kuribayashi

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  1. Toshiyuki Kitajima
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  2. Michihiro Iwashiro
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  3. Kagemasa Kuribayashi
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  4. Sadao Imamura
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Kitajima, T., Iwashiro, M., Kuribayashi, K. et al. Immunological characterization of tumor-rejection antigens on ultraviolet-light-induced tumors originating in the CB6F1 mouse. Cancer Immunol Immunother 38, 372–378 (1994). https://doi.org/10.1007/BF01517206

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  • DOI: https://doi.org/10.1007/BF01517206

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