Abstract
In intact mitochondria supplemented with succinate or β-hydroxybutyrate, the rates of oxygen consumption induced by beauvericin followed the ionic selectivity pattern: Na+>Rb+, Cs+, K+, Li+.
When the respiratory substrate is glutamate plus malate in the absence of phosphate, the selectivity pattern is: K+>Rb+>Cs+>Li+>Na+.
When the media are supplemented with phosphate, the Na+/K+ discrimination of beauvericin is considerably modified with all the respiratory substrates, being K+>Na+ with succinate and Na+>K+ with glutamate plus malate, whereas no significant ionic selectivity differences were obtained with β-hydroxybutyrate.
The respiratory control induced by oligomycin in submitochondrial particles is released by beauvericin only in the presence of a nigericin-like carboxylic antibiotic and an alkali metal cation, being far more effective in K+ than in Na+.
This selectivity is maintained regardless of whether NADH or succinate is used as a respiratory substrate.
Release of respiratory control can also be obtained with a combination of beauvericin and NH4Cl.
This information indicates that the ionic selectivity pattern obtained with beauvericin in mitochondrial membranes is an intrinsic property of the antibiotic which, however, can be significantly modified by factors such as the nature of the translocatable substrate anion or other anionic species, as well as the possible operation of a Na+/H+ antiporter existent in the membrane.
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References
H. A. Lardy, S. N. Graven and S. Estrada-O.,Fed. Proc.,26 (1967) 1355.
B. C. Pressman, in:Membranes of Mitochondria and Chloroplasts, E. Racker (ed), Van Nostrand-Reinhold, Princeton, New Jersey, 1969, p. 213.
B. Chance and M. Montal, in:Current Topics in Membranes and Transport, F. Bronner and A. Kleinzeller (eds), Academic Press, New York, 1971, Vol. 2, p. 99.
P. Mueller and D. O. Rudin, in:Current Topics in Bioenergetics, D. R. Sanadi (ed.), Academic Press, New York, 1969, Vol. 3, p. 157.
G. Eisenman, G. Szabo, S. G. A. McLaughlin and S. M. Ciani, in:Symposium on Molecular Mechanisms of Antibiotic Action on Protein Biosynthesis and Membranes, D. Vasquez (ed.), Springer-Verlag, 1971 (in press).
R. L. Hammil, H. E. Higgins, H. E. Boaz and M. Gorman.Tet. Letters,49 (1969) 4255.
E. Dorschner and H. A. Lardy,Antimicrobial Agents and Chemotherapy (1968) 11.
R. W. Roeske, S. Isaac, L. K. Steinrauf and T. King,Fed. Proc.,30 (1971) Part II, 1340.
Y. A. Ovchinnikov, V. T. Ivanov and I. I. Mikhaleva,Tet. Letters,2 (1971) 159.
D. Johnson and H. A. Lardy,Meth. Enzymol.,10 (1967) 94.
J. M. Fessenden and E. Racker,Meth. Enzymol.,10 (1967) 194.
B. C. Pressman,Proc. Nat. Acad. Sci. U.S.,53 (1965) 1066.
B. C. Pressman,Meth. Enzymol.,10 (1967) 714.
S. N. Graven, S. Estrada-O. and H. A. Lardy,Proc. Nat. Acad. Sci. U.S.,56 (1966) 654.
E. E. Jacobs, M. Jacob, D. R. Sanadi and L. B. Bradley,J. Biol. Chem.,223 (1956) 147.
B. C. Pressman, E. J. Harris, W. S. Jagger and J. H. Johnson,Proc. Nat. Acad. Sci. U.S.,58 (1967) 1949.
C. P. Lee and L. Ernster, in:Regulation of Metabolic Processes in Mitochondria, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.),Biochim. Biophys. Acta Library, 7 (1966) 218.
M. Montal, B. Chance and C. P. Lee,J. Membrane Biol.,2 (1970) 201.
S. Estrada-O., S. N. Graven and H. A. Lardy,Fed. Proc.,26 (1967) 610.
B. C. Pressman,Fed. Proc.,27 (1968) 1283.
S. Estrada-O., B. Rightmire and H. A. Lardy, in:Antimicrobial Agents and Chemotherapy, G. L. Hobby (ed.) (1967) 279.
S. Papa, in:The Energy-Level and Metabolic Control in Mitochondria, S. Papa, J. M. Tager, E. Quagliariello and E. C. Slater (eds.), Adriatica Editrice, Bari (1969) p. 273.
R. S. Cockrell and E. Racker,Biochem. Biophys. Res. Commun.,35 (1969) 414.
G. L. Sottocasa, B. Kuylenstierna, L. Ernster and A. Bergstrand,J. Cell. Biol.,32 (1967) 415.
P. Mitchell, in:Membranes and Transport, E. E. Bittar (ed.), Wiley, New York, 1970, Vol. 1, p. 192.
E. C. Slater,Quart. Rev. Biophys.,4 (1971) 35.
V. P. Skulachev, in:Current Topics in Bioenergetics, D. R. Sanadi (ed.), Academic Press, New York, 1971, Vol. 4, p. 127.
P. Mitchell and J. Moyle,Eur. J. Biochem.,9 (1969) 149.
M. Montal, B. Chance and C. P. Lee,Biochem. Biophys. Res. Commun.,36 (1969) 428.
J. B. Chappell and A. R. Crofts, in:Regulation of Metabolic Processes in Mitochondria, J. M. Tager, S. Papa, E. Quagliariello and E.C. Slater (eds.), Elsevier, Amsterdam,Biochim. Biophys. Acta Library 7 (1966) 293.
S. Estrada-O. and C. Gómez-Lojero,Biochemistry,10 (1971) 1598.
S. N. Graven, H. A. Lardy, D. Johnson and A. Rutter,Biochemistry,5 (1966) 1729.
C. Gómez-Lojero, S. Estrada-O. and M. Montal (1972) in preparation.
P. Mueller, D. O. Rudin, H. T. Tien and W. C. Wescott,J. Phys. Chem.,67 (1963) 534.
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Estrada-O, S., Gómez-Lojero, C. & Montal, M. Variability of the K+−Na+ discrimination of beauvericin in mitochondrial membranes. J Bioenerg Biomembr 3, 417–428 (1972). https://doi.org/10.1007/BF01516080
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DOI: https://doi.org/10.1007/BF01516080