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Inhibition of salvage pathway enzymes by adenine arabinoside 5′-monophosphate (ara-AMP)

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Summary

The effect of 9-β-arabinofuranosyladenine 5′-monophosphate (ara-AMP) on the purine salvage pathway has been studied. On a dose-dependant basis ara-AMP inhibits the incorporation of adenine-8-14C into nucleotides in intact erythrocytes. The partially purified enzymes of the purinc salvage pathway, the adenine phosphoribosyltransferase and the 5′-phosphoribosyl-1-pyrophosphate (PP-ribose-P) synthetase, but not the hypoxanthine-guanine phosphoribosyltransferase, are inhibited by ara-AMP in a non-competitive manner. The possible adverse drug interactions which might occur by the simultaneous use of ara-AMP and other antimetabolites are discussed.

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Abbreviations

A-PRT:

adenine phosphoribosyltransferase

Ara-A:

9-β-arabinofuranosyladenine

Ara-AMP:

9-β-arabinofuranosyladenine 5′-monophosphate

Ara-IIx:

hypoxanthine arabinoside

Ara-X:

xanthine arabinoside

HG-PRT:

hypoxanthine guanine phosphoribosyltransferase

PP-ribose-P:

5′-phosphoribosyl-1-pyrophosphatc

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Authors and Affiliations

  1. Department of Medicine, University of Freiburg i. Br., Germany

    H. J. Becher & P. Schollmeyer

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  1. H. J. Becher
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  2. P. Schollmeyer
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Dedicated to Prof. Dr. G.W. Löhr on the occasion of his 60th birthday

This study was supported by the Deutsche Forschungsgemeinschaft Be 458/4

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Becher, H.J., Schollmeyer, P. Inhibition of salvage pathway enzymes by adenine arabinoside 5′-monophosphate (ara-AMP). Klin Wochenschr 61, 751–757 (1983). https://doi.org/10.1007/BF01497402

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  • DOI: https://doi.org/10.1007/BF01497402

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