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Angiotensin II-Stoffwechsel bei Angiotensininfusion und bei Kochsalzentzug

Metabolism of angiotensin II during angiotensin infusion and following sodium depletion

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Summary

1. Angiotensin II (A II) and angiotensin (3–8)-hexapeptide (H) were measured by radioimmunoassay in arterial and venous plasma before and during infusion of angiotensin II-amide in five normotensive subjects, both in sodium-replete and sodium-depleted states. The separation of the oligopeptides was performed by thin layer caromatography.

2. Before sodium-depletion the mean ratio of arterial to venous A II was 1.14 ± 0.11:1, corresponding to an A II-extraction of approximately 12% during a single forearm passage. Infusion of A II-amide increased extraction of A II. The mean ratio of arterial to venous A II increased to 1.68 ± 0.3:1, corresponding to an extraction of approximately 39% when 8 ng·min−1·kg−1 were infused.

3. Sodium depletion increased arterial and venous concentrations of A II and H. During infusion of angiontensin II-amide the arterial and venous concentrations of A II and H increased approximately parallel to the concentrations before sodium depletion. The extraction of A II did not differ significantly in both states.

4. The augmented extraction of A II observed during infusion of angiotensin II-amide suggests an overproportional increase of the metabolism of A II under the latter condition. Sodium depletion, however, does not appear to cause a percent change in A II metabolism.

Zusammenfassung

1. Bei fünf normotonen Probanden wurde im arteriellen und venösen Plasma des Unterarms vor und nach diätetischem Salzentzug sowie vor und während Infusion von Angiotensin II-amid Angiotensin II (A II) und der Angiotensinmetabolit Angiotensin (3–8)-Hexapeptid (H) nach dünnschichtchromatographischer Trennung radioimmunologisch bestimmt.

2. Vor Salzentzug betrug das Verhältnis von arteriellem zu venösem A II-Spiegel 1,14 ± 0,11:1, entsprechend einer A II-Extraktion während einer einzigen Passage durch den Unterarm von etwa 12%. Die Infusion von Angiotensin II-amid führte zu einer Zunahme der Extraktion von A II; unter der höchsten von uns gewählten Infusionsgeschwindigkeit (8 ng·min−1·kg−1) stieg das Verhältnis von arteriellem zu venösem A II-Spiegel auf 1,68 ± 0,3:1, entsprechend einer A II-Extraktion von rund 39% an.

3. Salzentzug führte zu einem signifikanten Anstieg der arteriellen und venösen Konzentration von A II und H. Unter der Infusion von Angiotensin II-amid stieg die arterielle und die venöse A II-Konzentration von den erhöhten Ausgangswerten aus annähernd parallel zu den Konzentrationen vor Salzentzug an. Die Extraktion von A II unterschied sich nicht signifikant von derjeigen vor Salzentzug.

4. Die Zunahme der Extraktion von A II während der Angiotensin II-amid-Infusion, nicht jedoch während Kochsalzentzug zeigt eine überproportionale Steingerung des Angiotensinmetabolismus unter Infusionsbedingungen an. Unter Salzentzug tritt dagegen nach unseren Untersuchungen keine prozentuale Änderung des Angiotensinmetabolismus auf.

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Authors and Affiliations

  1. Medizinische Klinik der Universität Mainz, Germany

    H. P. Nast, U. Walter & A. Distler

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  1. H. P. Nast
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  2. U. Walter
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Herrn Prof. Dr. H.P. Wolff zum 60. Geburtstag gewidmet.

Mit finanzieller Unterstützung durch die Deutsche Forschungsgemeinschaft (Na 58/3) und durch den SFB 36 der Deutschen Forschungsgemeinschaft.

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Nast, H.P., Walter, U. & Distler, A. Angiotensin II-Stoffwechsel bei Angiotensininfusion und bei Kochsalzentzug. Klin Wochenschr 53, 623–628 (1975). https://doi.org/10.1007/BF01469682

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