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Enzymaktivitäten in normalen Thrombozyten verschiedener Dichte

Enzyme activities in normal platelets of different specific gravity

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Summary

Platelets of normal persons were separated into five fractions by centrifugation in discontinuous Ficoll density gradients. Enzyme activities of lactic dehydrogenase; phosphoglycerate kinase, glyceraldehyde phosphate dehydrogenase, pyruvate kinase, glucose-6-phosphate dehydrogenase and content of protein were constantly higher in the heavy fraction IV compared with the light fraction I. With regard to the platelet volume, however, these differences were compensated almost completely.

Zusammenfassung

Thrombozyten von Normalpersonen wurden durch diskontinuierliche Ficoll-Dichtegradienten-Zentrifugation in fünf verschiedene Fraktionen aufgetrennt. Die Enzymaktivitäten von Laktat-Dehydrogenase, Phosphoglyzerat-Kinase, Glyzeraldehyd-Phosphat-Dehydrogenase, Pyruvatkinase und Glukose-6-Phosphat-Dehydrogenase, sowie der Eiweißgehalt der Plättchen waren in der spezifisch schwereren Fraktion IV gegenüber der spezifisch leichten Fraktion I deutlich erhöht. Unter Berücksichtigung der Thrombozyten-Volumina glichen sich die Unterschiede jedoch nahezu vollständig aus.

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Authors and Affiliations

  1. Medizinische Universitätsklinik Tübingen, Germany

    O. Nerke, H. Chr. Benöhr, U. Thorau & H. D. Waller

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  1. O. Nerke
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  2. H. Chr. Benöhr
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  3. U. Thorau
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  4. H. D. Waller
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Mit freundlicher Unterstützung durch die Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg

Die Arbeit enthält Teile der Dissertation von Frau cand. med. U. Thorau

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Nerke, O., Benöhr, H.C., Thorau, U. et al. Enzymaktivitäten in normalen Thrombozyten verschiedener Dichte. Klin Wochenschr 55, 77–81 (1977). https://doi.org/10.1007/BF01469086

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