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Binding studies and localization ofEscherichia coli lipopolysaccharide in cultured hepatocytes by an immunocolloidal-gold technique

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Summary

In this study, the uptake and localization of anEscherichia coli lipopolysaccharide, and the temperature effects on these processes, were studied in rat cultured hepatocytes using a binding assay and an immunocolloidal gold technique. The lipopolysaccharide was found to bind to the cell membrane and microvilli after short incubation times, at both 4°C and 37°C. This was followed by a dispersed localization into the cytoplasm, reaching mitochondria. The uptake was found not to be receptor-mediated. A decrease of temperature, delays, but does not prevent, the lipopolysaccharide internalization.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Complutense, 28040, Madrid, Spain

    Inés Díaz-Laviada, Jesús Ainaga, M. Teresa Portolés, Angel M. Muncio & Raffaella Pagani

  2. Electron Microscopy Department, Centro de Biología Molecular, Universidad Autónoma, 28049, Madrid, Spain

    José L. Carrascosa

Authors
  1. Inés Díaz-Laviada
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  2. Jesús Ainaga
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  3. M. Teresa Portolés
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  4. José L. Carrascosa
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  5. Angel M. Muncio
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  6. Raffaella Pagani
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Díaz-Laviada, I., Ainaga, J., Teresa Portolés, M. et al. Binding studies and localization ofEscherichia coli lipopolysaccharide in cultured hepatocytes by an immunocolloidal-gold technique. Histochem J 23, 221–228 (1991). https://doi.org/10.1007/BF01462244

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  • DOI: https://doi.org/10.1007/BF01462244

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