Abstract
Rabbits were immunized with a low-density lipoprotein (LDL) preparation isolated from rabbit serum by ultracentrifugation. This elicited precipitin isoantibodies which distinguished two antigenically different genetic variants, i.e., allotypes of serum LDL. Both allotypes were identified as LDL by the following criteria: (1) the precipitin lines stained intensely with the lipid stain Sudan black B; (2) the antigens were found in the low-density but not the high-density lipoprotein fraction; (3) the antigens migrated electrophoretically on Agarose in the α 2 to β region. That the inheritance of these two allotypes is controlled by a pair of allelic genes at an autosomal locus is based on allotypes present in 323 progeny from six possible mating combinations. This LDL locus designated Lpqwas shown not to be linked to the light-chain or heavy-chain loci of immunoglobulins.
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This investigation was supported (in part) by NSF Grant GB-5536 and USPHS Grant AI07043-03.
Supported by USPHS predoctoral fellowship (1-F1-GM-37, 211-01) from the National Institute of General Medical Sciences.
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Albers, J.J., Dray, S. Identification and genetic control of two rabbit low-density lipoprotein allotypes. Biochem Genet 2, 25–35 (1968). https://doi.org/10.1007/BF01458449
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DOI: https://doi.org/10.1007/BF01458449