Skip to main content
SpringerLink
Log in

Biochemical and histochemical studies on the distribution of histamine in the digestive tract of man, dog and other mammals

  • Published:
Naunyn-Schmiedebergs Archiv für Pharmakologie Aims and scope Submit manuscript

Summary

  1. 1.

    The distribution of histamine was determined in tissues of the digestive tract of man, dog, pig, cow, and sheep, especially in the oral mucosa, stomach, gallbladder, and pancreas.

  2. 2.

    After treatment with compound 48/80, histamine was released from the frenulum linguae, soft palate, tongue, and thyroid gland of dogs, but not from the vestibulum oris, hard palate, pharynx, oesophagus, stomach, and pancreas (experiments in the dog). The release of histamine from the tongue showed regional differences and was lowest in the root and highest in the tip.

  3. 3.

    A parallelism could be shown between the histamine content and the mast cell density in different parts of the tongue, stomach and in the soft palate of untreated dogs and dogs treated with 48/80. The mast cells in the gastric mucosa could be characterized by their staining properties as “atypical” mast cells, whereas those in the musculature of the tongue were “typical” mast cells.

  4. 4.

    The histamine content of the single mast cell was similar in all tissues (3.2 pg/ cell in the tongue, 3.3 in the stomach, 4.8 in the soft palate and 3.4 in the submaxillary gland). Only the mast cells in the fundic mucosa showed a significantly lower histamine content (1.9 pg/cell). The mast cells of the fundus and body of the stomach of the dog seemed to store histamine which was released by 48/80.

  5. 5.

    A new classification of histamine stores is proposed: “unspecific mast cell stores” and “tissue specific stores”.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arsdel, P. P. van, Jr., andR. E. Bray: On the release of histamine from rat peritoneal mast cells by compound 48/80: Effects of metabolic inhibitors. J. Pharmacol. exp. Ther.133, 319 (1961).

    PubMed  Google Scholar 

  • Arvy, L., etD. Quivy: Relation entre la richesse en labrocytes et la teneur en histamine des divers segments du tube digestif chez le chien. C. R. Soc. Biol. (Paris)149, 658 (1955).

    Google Scholar 

  • — —: Dormées sur la répartition des labrocytes chez le chien. C. R. Ass. Anat.42, 234 (1955a).

    Google Scholar 

  • Aures, D., R. Håkanson, Ch. Owman, andB. Sporrong: Cellular stores of histamine and monamines in the dog stomach. Life Sci.7, 1147 (1968).

    Article  Google Scholar 

  • Boréus, L. O., andN. Chakravarty: The histamine content of guinea-pig mast cells. Experientia (Basel)16, 192 (1960).

    Google Scholar 

  • Brodie, B. B., M. A. Beaven, F. Erjevec, andH. L. Johnson: Uptake and release of H3-histamine. In: Mechanisms of release of biogenic amines, Wenner-Gren Center International Symposium Series, Vol. 5, p. 401. Oxford: Pergamon Press 1966.

    Google Scholar 

  • Code, C. F.: The source in blood of the histamine like contituent. J. Physiol. (Lond.)90, 349 (1937).

    Google Scholar 

  • —: Histamine and gastric secretion a later look 1955–1965. Fed. Proc.25, 1311 (1965).

    Google Scholar 

  • Ehinger, B., R. Håkanson, Ch. Owman, andB. Sporrong: Histochemical demonstration of histamine in paraffin sections by a fluorescence method. Biochem. Pharmacol.17, 1997 (1968).

    Article  PubMed  Google Scholar 

  • Ellenberger, W., u.H. Baum: Handbuch der vergleichenden Anatomie der Haustiere, S. 419. Berlin: A. Hirschwald 1912.

    Google Scholar 

  • Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. 1. Effects of fixation. Acta path. microbiol. scand.66, 289 (1966a).

    PubMed  Google Scholar 

  • —: Mast cells in rat gastrointestinal mucosa. 2. Dye-binding and metachromatic properties. Acta path. microbiol. scand.66, 303 (1966b).

    PubMed  Google Scholar 

  • —: Mast cells in rat gastrointestinal mucosa. 3. Reactivity towards compound 48/80. Acta path. microbiol. scand.66, 313 (1966c).

    PubMed  Google Scholar 

  • —: Mast cells in rat gastrointestinal mucosa. 4. Monoamine storing capacity. Acta path. microbiol. scand.67, 365 (1966d).

    PubMed  Google Scholar 

  • Feldberg, W., andG. W. Harris: Distribution of histamine in the mucosa of the gastro-intestinal tract of the dog. J. Physiol. (Lond.)120, 352 (1953).

    Google Scholar 

  • —, andJ. Talesnik: Reduction of tissue histamine by compound 48/80. J. Physiol. (Lond.)120, 550 (1953).

    Google Scholar 

  • Gastpar, H., u.W. Lorenz: Speicherung, Bildung und Umsatz von Histamin in Speicheldrüsen und Tonsillen. Arch. klin. exp. Ohr.-, Nas.-u. Kehlk. Heilk.191, 715 (1968).

    Google Scholar 

  • Graham, H. T., D. H. Lowry, N. Wahl, andM. K. Priebat: Mast cells as sources of tissue histamine. J. exp. Med.102, 307 (1955).

    Article  PubMed  Google Scholar 

  • Håkanson, R., B. Lilja, andCh. Owman: Properties of a new system of amine-storing cells in the gastric mucosa of the rat. Europ. J. Pharmacol.1, 188 (1967a).

    Article  Google Scholar 

  • — — —, andS. Thunell: Changes in gastric secretion induced by certain amines, amine precursors and related enzyme inhibitors. Europ. J. Pharmacol.1, 425 (1967b).

    Article  Google Scholar 

  • —,Ch. Owman, andN. O. Sjöberg: Cellular stores of gastric histamine in the developing rat. Life Sci.6, 2535 (1967).

    Article  PubMed  Google Scholar 

  • Juhlin, L., andW. B. Shelley: Detection of histamine by a new fluorescent o-phthalaldehyde stain. J. Histochem. Cytochem.14, 525 (1966).

    PubMed  Google Scholar 

  • Kataoka, K., andE. De Robertis: Histamine in isolated small nerve endings and synaptic vesicles of rat brain cortex. J. Pharmacol. exp. Ther.156, 114 (1967).

    PubMed  Google Scholar 

  • Lorenz, W.: Lokalisation, Stoffwechsel und Frage einer physiologischen Funktion von Histamin in Speicheldrüsen, Schilddrüse und Magen. Habilitationsschrift, München 1969.

  • —,M. Gerant, andE. Werle: Induction of the specific histidine decarboxylase by TSH and 2-mercaptobenzimidazole-1,3-dimethylol (MBI). Naunyn-Schmiedebergs Arch. Pharmak. exp. Path.260, 171 (1968a).

    Article  Google Scholar 

  • - St.Halbach, M.Gerant, and E.Werle: Specific histidine decarboxylases in the gastric mucosa of man and other mammals: Determination, location and properties. Biochem. Pharmacol. (in press) (1969).

  • —,G. Haubensak, M. Hutzel u.E. Werle: Histaminliberierung in Gl. submaxillaris und Pankreas durch Parasympathicomimetica, Peptidhormone, Histamin und Mepyramin. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path.260, 416 (1968b).

    Article  Google Scholar 

  • —,St. Heitland, A. Schauer, H. Gastpar u.E. Werle: Histamin in Speicheldrüsen, Tonsillen und Thymes und adaptative Histaminbildung in der Gl. submaxillaris. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path.259, 319 (1968c).

    Article  Google Scholar 

  • - M.Hutzel, E.Matekja, and E.Werle: In preparation.

  • —, u.K. Pfleger: Stoffwechsel und physiologische Funktion von Histamin im Magen. Klin. Wschr.46, 57 (1968d).

    Google Scholar 

  • — —, u.E. Werle: In vitro-Hemmung der Histidindecarboxylasen durch antithyreoidale Substanzen. Biochem. Pharmacol.17, 539 (1968e).

    Article  PubMed  Google Scholar 

  • -, and E.Werle: Inhibition of the salivary secretion by antihistaminic drugs and the question of a physiological rôle of histamine in the submaxillary and parotid glands, p. 362. Abstr. Intern. Congress of Pharmacology, Basel (1969a).

  • — —: Occurrence, cellular distribution and subcellular localization of histamine in man, animals and plants. Int. Encyclopedia of Pharmacology and Therapeutics. Oxford: Pergamon Press 1969 (in press).

    Google Scholar 

  • MacIntosh, F. C., andW. D. M. Paton: The liberation of histamine by certain organic bases. J. Physiol. (Lond.)109, 190 (1949).

    Google Scholar 

  • Minard, D.: The presence and distribution of histamine in blood. Amer. J. Physiol.132, 327 (1941).

    Google Scholar 

  • Mota, I., W. T. Beraldo, A. G. Ferri, andL. C. U. Junqueira: Intracellular distribution of histamine. Nature (Lond.)174, 698 (1954).

    Google Scholar 

  • —,A. G. Ferri, andS. Yoneda: The distribution of mast-cells in the digestive tract of laboratory animals: its bearings on the problem of the location of histamine in tissues. Quart. J. micr. Sci.97, 251 (1956).

    Google Scholar 

  • Riley, J. F.: The mast cells. Edinburgh-London: E. &. S. Livingstone 1959.

    Google Scholar 

  • —, andG. B. West: The presence of histamine in tissue mast cells. J. Physiol. (Lond.)120, 528 (1953).

    Google Scholar 

  • Schauer, A.: The mast cell. In: Veröffentlichungen aus der morphologischen Pathologie,F. Buchner undW. Giese (Eds.). Stuttgart: G. Fischer 1964.

    Google Scholar 

  • Schauer, A., u.E. Werle: Zur histochemischen Darstellung des Histamins der Mastzellen. Z. ges. exp. Med.131, 100 (1959).

    PubMed  Google Scholar 

  • Schayer, R. W.: Formation and binding of histamine by free mast cells of rat peritoneal fluid. Amer. J. Physiol.196, 189 (1956).

    Google Scholar 

  • —: Evidence that induced histamine is an intrinsic regulator of the microcirculatory system. Amer. J. Physiol.202, 66 (1962).

    PubMed  Google Scholar 

  • Selye, H.: The mast cells, p. 95. London: Butterworths 1965.

    Google Scholar 

  • Shore, P. A., A. Burkhalter, andV. H. Cohn, Jr.: A method for the fluorometric assay of histamine in tissues. J. Pharmacol. exp. Ther.127, 182 (1959).

    PubMed  Google Scholar 

  • Snyder, S. H., J. Glowinski, andJ. Axelrod: The physiological disposition of H3-histamine in the rat brain. J. Pharmacol. exp. Ther.153, 8 (1966).

    PubMed  Google Scholar 

  • Thunberg, R.: Localization of cells containing and forming histamine in the gastric mucosa of the rat. Exp. Cell Res.47, 108 (1967).

    Article  PubMed  Google Scholar 

  • Uvnäs, B., andI. L. Thon: A physico-chemical model of histamine release from mast cells. In: Mechanisms of release of biogenic amines. Proc. International Wenner Gren Center Symposium Series, Vol. 5, p. 361. Oxford: Pergamon Press 1966.

    Google Scholar 

  • Weinshelbaum, E. J., andD. J. Ferguson: Localization of carbon-14 from histamine in enterochromaffin cells. Gastroenterology51, 1028 (1968).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Klinische Chemie und Klinische Biochemie and Pathologisches Institut der Universität München, Deutschland

    W. Lorenz, A. Schauer, St. Heitland, R. Calvoer & E. Werle

Authors
  1. W. Lorenz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Schauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. St. Heitland
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Calvoer
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Werle
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant from Deutsche Forschungsgemeinschaft.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lorenz, W., Schauer, A., Heitland, S. et al. Biochemical and histochemical studies on the distribution of histamine in the digestive tract of man, dog and other mammals. Naunyn-Schmiedebergs Arch. Pharmak. 265, 81–100 (1969). https://doi.org/10.1007/BF01417211

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01417211

Key-words

Search

Navigation