Abstract
A non-uniform distribution of ionic groups on the surface of a protein can lead to attractive electrostatic interactions between itself and an adsorbent, even when the net charge of the protein is of the same type as that of the adsorbent. Chromatographic retention mapping as a function of pH and ionic strength is employed to identify electrostatic repulsion or attraction. Thus, it can be determined whether the global characteristics or an oppositely charged patch controls the adsorptive behavior of a protein on a charged adsorbent.
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Lesins, V., Ruckenstein, E. Patch controlled attractive electrostatic interactions between similarly charged proteins and adsorbents. Colloid & Polymer Sci 266, 1187–1190 (1988). https://doi.org/10.1007/BF01414409
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DOI: https://doi.org/10.1007/BF01414409