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Two polypeptides (30 and 38kDa) in plant coated vesicles with clathrin light chain properties

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Summary

Coated vesicles have been isolated from bovine brain and etiolated zucchini hypocotyls by centrifugal methods. By putting to use two properties of the light chain polypeptides of brain coated vesicles (calcium binding, heat stability) we have been able to demonstrate the presence of two similar polypeptides with apparent molecular masses of 30 and 38 kDa in plant coated vesicles.

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Abbreviations

CV:

coated vesicle

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

MES:

2-(N-Morpholino)-ethanesulfonic acid

Tris:

tris(hydroxymethyl) aminomethane

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Authors and Affiliations

  1. Plant Physiology Institute, University of Göttingen, Germany

    K. Balusek, H. Depta & D. G. Robinson

Authors
  1. K. Balusek
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  2. H. Depta
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  3. D. G. Robinson
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Balusek, K., Depta, H. & Robinson, D.G. Two polypeptides (30 and 38kDa) in plant coated vesicles with clathrin light chain properties. Protoplasma 146, 174–176 (1988). https://doi.org/10.1007/BF01405927

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  • DOI: https://doi.org/10.1007/BF01405927

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