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Induction and formation ofCochliobolus sativus appressoria

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Summary

GerminatingCochliobolus sativus spores were induced to form appressoria on a variety of artificial surfaces, including replicas of the barley leaf surface. Evidence was obtained for the involvement of chemical and topographic signals during induction of appressorium formation inC. sativus. Germ tube thigmotropism was also observed in vitro. Ultrastructure relevant to appressorium formation was observed, including the germ tube apex, apical swelling of the germ tube apex prior to appressorium formation, the appressorium with associated septation and the penetration peg. Cytochemical probes applied to germlings at the electron microscope level failed to detect α-D-mannan, α-D-glucan, β-D-galactan, D-glcNAc or D-galNAc polymers in the extracellular mucilage associated with the fungal germlings. The ultrastructure of hyphal apices from germlings grown under different nutritional conditions differed with respect to Spitzenkörper morphology, apex shape and in the quantity of associated extracellular mucilage. Experimental findings are discussed relative to current understanding of appressorium induction in more extensively studied systems.

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Abbreviations

PDA:

potato dextrose agar

DS:

dilute salts

Con:

A concanavalin A

RcA120 :

Ricinus communis agglutinin120

WGA:

wheat germ agglutinin

HpA:

Helix pomatia agglutinin

DIC:

differential interference contrast

UV:

ultraviolet

TEM:

transmission electron microscopy

NNF:

National Nanofabrication Facility

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Authors and Affiliations

  1. Department of Botany, University of Georgia, 30602, Athens, GA, USA

    R. P. Clay, J. Enkerli & M. S. Fuller

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  1. R. P. Clay
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  2. J. Enkerli
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  3. M. S. Fuller
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Clay, R.P., Enkerli, J. & Fuller, M.S. Induction and formation ofCochliobolus sativus appressoria. Protoplasma 178, 34–47 (1994). https://doi.org/10.1007/BF01404119

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  • DOI: https://doi.org/10.1007/BF01404119

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