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Ultrastructural changes induced by experimental subarachnoid haemorrhage and 6-hydroxydopamine in cat cerebral arteries

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Summary

Transmission electron microscopy of the middle cerebral artery from cats exposed to subarachnoid injection of blood 3 and 7 days before, showed several damage of the vascular ultrastructure. The intima was thickened with swelling and vacuolization of endothelial cells, with a plump appearance and disruption of tight junctions. The cellular surface was corrugated and the subendothelial space contained proliferating and vacuolated smooth muscle cells capped by elastin and collagen fibres. The internal elastic lamina was also corrugated and disrupted. The adventitial changes were axonic cytoplasmic and mitochondrial swelling, virtual absence of synaptic vesicles, and disruption of the outer axonal membrane, suggesting denervation of cerebral vessels. With administration of 6-hydroxydopamine (6-OHDA), similar ultrastructural changes were observed in the adventitia. These observations indicate that denervation induced by subarachnoid bleeding could be involved in the pathophysiology of cerebrovascular spasm. Subarachnoid haemorrhage, but not 6-OHDA, affects also intima and tunica media, suggesting other mechanisms, in addition to denervation, can participate in the vasospasm.

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References

  1. Aprigliano O, Rybarczyck KE, Hermsmeyer K, Van Orden LS (1976) Adrenergic neuronal degeneration induced in portal vein and caudal artery by 6-hydroxydopamine in vitro. J Pharmacol Exp Ther 198: 578–588

    PubMed  Google Scholar 

  2. Bevan JA, Brayden JE (1987) Noradrenergic neural vasodilator mechanisms. Circ Res 60: 309–326

    PubMed  Google Scholar 

  3. Chyatte D, Rusch N, Sundt Jr TM (1983) Prevention of chronic experimental cerebral vasospasm with ibuprofen and high-dose metylprednisolone. J Neurosurg 59: 925–932

    PubMed  Google Scholar 

  4. Duff TA, Scott G, Feilbach JA (1986) Ultrastructural evidence of arterial denervation following experimental subarachnoid hemorrhage. J Neurosurg 64: 292–297

    PubMed  Google Scholar 

  5. Duff TA, Louie J, Feilbach JA, Scott G (1988) Erythrocytes are essential for development of cerebral vasculopathy resulting from subarachnoid hemorrhage in cats. Stroke 19: 68–72

    PubMed  Google Scholar 

  6. Eldevik OP, Kristiansen K, Torvik A (1981) Subarachnoid hemorrhage on cerebrovascular spasm. Morphological study of intracranial arteries based on animal experiments and human autopsies. J Neurosurg 55: 869–876

    PubMed  Google Scholar 

  7. Emmelin N, Trendelenburg U (1972) Degeneration activity after parasympathetic or sympathetic denervation. Erberg Physiol 66: 147–121

    Google Scholar 

  8. Espinosa E, Weir B, Boisvert D, Overton T, Casta W (1982) Chronic cerebral vasospasm after large subarachnoid hemorrhage in monkeys. J Neurosurg 57: 224–232

    PubMed  Google Scholar 

  9. Espinosa E, Weir B, Shnitka T, Overton T, Boisvert D (1984) A randomized placebo-controlled double blind trial nimodipine after SAH in monkeys. Part 2: Pathologic findings. J Neurosurg 60: 1176–1185.

    PubMed  Google Scholar 

  10. Furness JB, Campbell GR, Gillard SM, Malmfors T, Cobb JLS, Burnstock G (1970) Cellular studies of sympathetic denervation produced by 6-hydroxydopamine in the vas deferens. J Pharmacol Exp Ther 174: 111–122

    PubMed  Google Scholar 

  11. Hara H, Nosko M, Weir B (1986) Cerebral perivascular nerves in subarachnoid hemorrhage. A histochemical and immunohistochemical study. J Neurosurg 65: 531–539

    PubMed  Google Scholar 

  12. Harder DR, Dernbach P, Waters A (1987) Possible cellular mechnism for cerebral vasospasm after experimental subarachnoid hemorrhage in the dog. J Clin Invest 80: 875–880

    PubMed  Google Scholar 

  13. Heros RC, Zervas NT, Varsoz V (1983) Cerebral vasospasm after subarachnoid hemorrhage. An update. Ann Neurol 13: 599–608

    Google Scholar 

  14. Jonsson G, Sachs Y (1972) Degenerative and non degenerative effects of 6-hydroxydopamine on adrenergic nerves. J Pharmacol Exp Ther 180: 625–635

    PubMed  Google Scholar 

  15. Lee TJF, Saito A, Berezin I (1984) Vasoactive intestinal polypeptide-like substance: the potential transmitter for cerebral vasodilation. Science 224: 898–901

    PubMed  Google Scholar 

  16. Liszczak TM, Varsos VG, Black PM, Kistler P, Zervas NT (1983) Cerebral arterial constriction after experimental subarachnoid hemorrhage is associated with blood components within the arterial wall. J Neurosurg 58: 18–26

    PubMed  Google Scholar 

  17. Lobato RD, Marin J, Salaices M, Burgos J, Rivilla F (1980) Cerebrovascular reactivity to noradrenaline and serotonin following experimental subarachnoid hemorrhage. J Neurosurg 53: 480–485

    PubMed  Google Scholar 

  18. Lobato RD, Marin J, Salaices M, Burgos J, Rivilla F, Garcia AG (1980) Effect of experimental subarachnoid hemorrhage on the adrenergic innervation of cerebral arteries. J Neurosurg 53: 477–479

    PubMed  Google Scholar 

  19. Martin W, Villani GM, Jothianandan D, Furchgott RF (1985) Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. J Pharmacol Exp Ther 232: 708–716

    PubMed  Google Scholar 

  20. Mayberg MR, Houser OW, Sundt TM (1976) Ultrastructural changes in feline arterial endothelium following subarachnoid hemorrhage. J Neurosurg 48: 49–57

    Google Scholar 

  21. Nakagomi T, Kassell NF, Sasaki T, Fujiwara S, Lehman M, Johshita H, Nazar GB, Torner JC (1987) Effect of subarachnoid hemorrhage on endothelium dependent vasodilation. J Neurosurg 66: 915–923

    PubMed  Google Scholar 

  22. Nakagomi T, Kassell NF, Sasaki T, Fujiwara S, Lehman M, Johshita H, Nazar GB, Torner JC (1987) Impairment of endothelium-dependent vasodilation induced by acetylcholine and adenosine triphosphate following experimental subarachnoid hemorrhage. Stroke 18: 482–489

    PubMed  Google Scholar 

  23. Sakaki T, Tanigake T, Kyoki K (1979) Pathological study of late arterial spasm. Neurol Med Chir 19: 1085–1093

    Google Scholar 

  24. Smith RR, Clower BR, Grotendorst GM, Yabuno N, Cruse JM (1985) Arterial wall changes in early human vasospasm. Neurosurgery 16: 171–176

    PubMed  Google Scholar 

  25. Takemae T, Branson J, Alksne JF (1984) Intimai proliferation of cerebral arteries after subarachnoid blood injections in pigs. J Neurosurg 61: 494–500

    PubMed  Google Scholar 

  26. Vanhoutte PM, Rubanyi GM, Miller VM (1986) Modulation of vascular smooth muscle contraction by the endothelium. Ann Rev Physiol 48: 307–320

    Google Scholar 

  27. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazak Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 410–415

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, Autonomous University, Madrid, Spain

    F. Rivilla, J. Marín, C. F. Sánchez-Ferrer & M. Salaices

  2. Department of Morphology, School of Medicine, Autonomous University, Madrid, Spain

    F. Rivilla, J. Marín, C. F. Sánchez-Ferrer, M. Salaices & P. G. Ramos

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  1. F. Rivilla
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  2. J. Marín
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  3. C. F. Sánchez-Ferrer
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  4. M. Salaices
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  5. P. G. Ramos
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Rivilla, F., Marín, J., Sánchez-Ferrer, C.F. et al. Ultrastructural changes induced by experimental subarachnoid haemorrhage and 6-hydroxydopamine in cat cerebral arteries. Acta neurochir 100, 158–163 (1989). https://doi.org/10.1007/BF01403605

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