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Production and biological activity of saponins and canavanine in alfalfa seedlings

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Abstract

The saponin and canavanine concentrations and pattern were analyzed in growing alfalfa seedlings (Medicago sativa L.). Accumulation of saponins and canavanine was found to follow different time courses. During the first eight days, saponin concentration rose from zero in alfalfa seeds to 8.7% in roots and 1.8% in shoots on eighth day and then slowly decreased to 7.6% in roots and 0.8% in shoots present on the 24th day. Canavanine was found in seeds at a concentration of 1 % then increased to 3.2% in seedlings on the sixth day and rapidly decreased to 0.2% per dry mass in roots and shoots on the 24th day. The effect of saponins-medicagenic acid sodium salt and medicagenic acid glycosides-on the growth ofAmaranthus andLepidium in Petri dishes and tomato (Lycopersicon) cell growth in tissue culture also was investigated. In contrast to medicagenic acid glycosides, a very strong inhibition of plant and cell growth was found as an effect of medicagenic acid.

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References

  • Abdul-Rahman, A.A. 1989. Allelopathic effect of alfalfa (Medicago sativa) on blady grass (Imperata cylindrica)J'. Chem. Ecol. 15:2289–2301.

    Google Scholar 

  • Assa, Y., Chet, J., Gestetner, B., Govrin, R., Birk, Y., andBondi, A. 1975. The effect of alfalfa saponins on growth and lysis ofPhysarum polycephalum.Arch. Microbiol. 103:77–80.

    PubMed  Google Scholar 

  • Bell, E.A. 1980. The non-protein amino acids of higher plants.Endeavour N.S. 4:102–107.

    Google Scholar 

  • Birk, Y. 1969. Saponins, pp. 169–210,in I.E. Liener (ed.). Toxic Constituents of Plant Foodstuffs. Academic Press New York.

    Google Scholar 

  • Gestetner, B., Assa, Y., Henis, Y., Birk, Y., andBondi, A. 1971. Lucerne saponins. IV. Relationship between their chemical constitution and haemolytic and antifungal activity.J. Sci. Food. Agric. 22:168–172.

    PubMed  Google Scholar 

  • Gorski, P.M., Jurzysta, M., Burda, S., Oleszek, W., andPloszynski, M. 1984. Studies onMedicago lupulina saponins. 2. Isolation, chemical characterization and biologically activity of saponins fromM. lupulina tops.Acta Soc. Bot. Pol. 53:527–533.

    Google Scholar 

  • Grancharov, K., Krauss, G.-J., Spassovska, N., Miersch, J., Maneva, L., Mladenova, L., andGolovinsky, E. 1985. Inhibitory effect of pyruvic acid semi- and thiosemicarbazone on the germination of bacteria, yeasts, experimental tumors and plant cells.Pharmazie 40:574–575.

    PubMed  Google Scholar 

  • Jurzysta, M. 1970. Effect of saponins isolated from seeds of lucerne on germination and growth of cereal seedlings.Zesz. Nauk UMK Torun 13:253–256.

    Google Scholar 

  • Jurzysta, M. 1979a. Simple method of quantification of biologically active alfalfa saponins byTrichoderma viride growth.Biul. Branz. Hod. Roslin 1:16–18.

    Google Scholar 

  • Jurzysta, M. 1979b. Haemolytic micromethod for rapid estimation of toxic alfalfa saponins.Acta Agrob. 32:5–11.

    Google Scholar 

  • Miersch, J., Jühlke, C., andSchlee, D. 1988. Canavaninmetabolismus während der Sämlingsentwicklung von Luzerne (Medicago sativa L. cv. Verko).Wiss. Beitr. Martin-Luther-Univ. Halle-Wittenberg 33(S 65):62–71.

    Google Scholar 

  • Miersch, J.,Jühlke, C.,Kabat Vel Job, C., andSchlee, D. 1990. Canavanine as an allelochemical in the seedlings of alfalfa. Proc. 14th Intern. Congr. of Biochemistry, Prague, 1988. In press.

  • Natelson, S., andBratton, G.R. 1984. Canavanine assay of some alfalfa varieties (Medicago saliva) by fluorescence: Practical procedure for canavanine preparation.Microchem. J. 29:26–43.

    Google Scholar 

  • Oleszek, W., andJurzysta, M. 1986. Isolation, chemical characterization and biologically activity of alfalfa (Medicago media Pers.) root saponins.Acta Soc. Bot. Pol. 55:23–33.

    Google Scholar 

  • Oleszek, W., andJurzysta, M. 1987. The allelopathic potential of alfalfa root medicagenic acid glycosides and their fate in soil environments.Plant Soil 98:67–80.

    Google Scholar 

  • Pfüller, K. 1976. Isolierung, Analytik und Biochemie toxischer Inhaltsstoffe der Luzerne (Medicago sativa L.). Dissertation A, Math.-Nat.-Fak. University of Leipzig.

  • Polacheck, J., Zehavi, U., Naim, M., Levy, M., andEvron, R. 1986. Activity of compound G2 isolated from alfalfa roots against medically important yeasts.Antimicrob. Agents Ther. 30:290–294.

    Google Scholar 

  • Read, J.J., andJensen, E.H. 1989. Phytotoxicity of water-soluble substances from alfalfa and barley soil extracts on four crop species.J. Chem. Ecol. 15:619–628.

    Google Scholar 

  • Ream, H.W., Smith, D., andWalgenbach, R.P. 1977. Effect of deproteinized alfalfa juice applied to alfalfa-bromograss, bromegrass and corn.Agron. J. 69:685–689.

    Google Scholar 

  • Rosenthal, G.A. 1973. The preparation and colorimetric analysis ofl-canaline.Anal. Biochem. 51:354–361.

    PubMed  Google Scholar 

  • Rosenthal, G.A. 1977. The biological effects and mode of action ofl-canavanine a structural analogue ofl-arginine.Rev. Biol. 52:155–178.

    Google Scholar 

  • Rosenthal, G.A. 1982. Plant Non-protein Amino- and Imino Acids. Biological, Biochemical, and Toxicological Properties. Academic Press New York, 273 pp.

    Google Scholar 

  • Rosenthal, G.A. 1983. A seed-eating beetle's adaptations to a poisonous seed.Sci. Am. 249:138–345.

    Google Scholar 

  • Rosenthal, G.A., Gulati, D.K., andSabharwal, P.S. 1975. Studies on the growth effects of the canaline urea cycle amino acids withLemna minorL.Plant Physiol. 56:420–424.

    Google Scholar 

  • Slotwinska, M. 1983. The effect of alfalfa saponins on L cells cultured in vitro.Ann. Univ. Maria Curie-Slodowska Sect. C Biol. 38:177–182.

    Google Scholar 

  • Wilson, M.F., andBell, E.A. 1978. The determination of the changes in the free amino acid content of the eluate from germinating seeds ofGlycine wightii (L.) and its effect on the growth of lettuce fruits.J. Exp. Bot. 29:1243–1247.

    Google Scholar 

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Gorski, P.M., Miersch, J. & Ploszynski, M. Production and biological activity of saponins and canavanine in alfalfa seedlings. J Chem Ecol 17, 1135–1143 (1991). https://doi.org/10.1007/BF01402939

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  • DOI: https://doi.org/10.1007/BF01402939

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