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Detection of human polyomavirus DNA in urine specimens by hybridot assay

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Summary

A hybridot assay method using a labelled BK virus probe has been used to detect the presence of human polyomavirus DNA in 81 urine specimens from 61 patients, most of whom were immuno-compromised to some degree. Twenty-eight urines from 23 patients had detectable DNA. The results have been compared to virus isolation and electron microscopy on the same specimens. In 90 per cent a comparable result was obtained by at least one of the other two tests and in the 73 specimens on which all 3 tests were performed there was 80 per cent agreement between all. In 6 cases the hybridot assay was more sensitive in detecting infection.

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Authors and Affiliations

  1. Virus Reference Laboratory, Central Public Health Laboratory, London, England, United Kingdom

    P. E. Gibson, S. D. Gardner & A. A. Porter

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  1. P. E. Gibson
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  2. S. D. Gardner
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  3. A. A. Porter
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Gibson, P.E., Gardner, S.D. & Porter, A.A. Detection of human polyomavirus DNA in urine specimens by hybridot assay. Archives of Virology 84, 233–240 (1985). https://doi.org/10.1007/BF01378975

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  • DOI: https://doi.org/10.1007/BF01378975

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