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Postmortem changes in hapten-specific IgE antibody responses in mice

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Summary

This Study was carried out to examine the post-mortem stability of hapten-specific immunoglobulin E (IgE) antibody in mice by the passive cutaneous anaphylaxis test in rats. In vitro IgE antibody responses in blood were stable for 3 days at room temperature. Even in cases when blood could not be obtained from the caver it was possible to measure the hapten-specific IgE antibody response up to 3 days after death using the supernatant of the visceral homogenates.

Zusammenfassung

Die Untersuchung wurde durchgeführt, um mit Hilfe des passiven kutanen Anaphylaxie-Tests bei Ratten die postmortale Stabilität des haptenspezifischen Immunoglobulins E von Mäusen zu überprüfen. In-vitro IgE-Antikörper-Antworten waren — unter Raumtemperatur-Bedingungen — 3 Tage unverändert. Selbst in Fällen, wo Blut vom Kadaver nicht gewonnen werden konnte, war es möglich, die hapten-spezifische IgE-Antikörper-Antwort bis zu 3 Tagen nach dem Tode zu bestimmen, indem der Überstand von Organ-Homogenaten verwendet wurde.

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Authors and Affiliations

  1. Department of Legal Medicine, Fukuoka University School of Medicine, Fukuoka 814-01, 7-45-1 Nanakuma, Jonan-ku, Japan

    Y. Heida, M. Kaguera, K. Hara & S. Kashimura

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  1. Y. Heida
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  2. M. Kaguera
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  3. K. Hara
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  4. S. Kashimura
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Heida, Y., Kaguera, M., Hara, K. et al. Postmortem changes in hapten-specific IgE antibody responses in mice. Int J Leg Med 104, 133–135 (1991). https://doi.org/10.1007/BF01369716

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  • DOI: https://doi.org/10.1007/BF01369716

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