Abstract
Population studies on six short tandem repeat loci, HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 were carried out in a sample of unrelated Japanese individuals (n = 337–545) living in Gifu Prefecture (central region of Japan). Five alleles could be identified for HUMFXIIIB, six for HUMF13A01, HUMLIPOL, HUMTH01 and HUMTPOX, and eight for HUMVWFA31. For all/six loci no deviations from the Hardy-Weinberg equilibrium hypothesis were detected. The mean exclusion chance ranged from 0.22 to 0.60, the power of discrimination from 0.63 to 0.93, and the expected heterozygosity from 0.43 to 0.80. Allele frequency distributions for the loci in the Japanese sample were not similar to those in samples from other racial or ethnic groups except for the Chinese (for HUMTPOX). The results demonstrate that HUMTH01, HUMTPOX and HUMVWFA31 are more useful for forensic investigations in the Japanese population than the other three loci.
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Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the VNTR locus DIS80 by the PCR followed by high-resolution PAGE. Am J Hum Genet 48:137–144
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12:241–253
Fisher RA (1951) Standard calculations for evaluating a blood group system. Heredity 5:95–102
Fujita H, Naito E, Dewa K, Yamanouchi H (1995) Three short tandem repeat loci in Japanese population. Jpn J Legal Med 49:213–221
Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55:175–189
Hochmeister MN, Budowle B, Schumm JW, Sprecher CJ, Borer UV, Dimhofer R (1995) Swiss population data and forensic efficiency values on 3 tetrameric short tandem repeat loci — HUMTH01, TPOX, and CSF1PO — derived using a STR multiplex system. Int J Legal Med 107:246–249
Huang NE, Schumm J, Budowle B (1995) Chinese population data on three tetrameric short tandem repeat loci — HUMTH01, TPOX, and CSFIPO — derived using multiplex PCR and manual typing. Forensic Sci Int 71:131–136
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Meyer E, Wiegand P, Brinkmann B (1995) Phenotype differences of STRs in 7 human populations. Int J Legal Med 107:314–322
Möller A, Wiegand P, Grüchow C, Seuchter SA, Baur MP, Brinkmann B (1994) Population data and forensic efficiency values for the STR systems HumVWA, HumMBP and HumFABP. Int J Legal Med 106:183–189
Ohno Y, Sebetan IM, Akaishi S (1982) A simple method for calculating the probability of excluding paternity with any number of codominant alleles. Forensic Sci Int 19:93–98
Pestoni C, Lareu MV, Rodríguez MS, Muñoz I, Barros F, Carracedo A (1995) The use of STRs HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS, HUMLPL in forensic application: validation studies and population data for Galicia (NW Spain). Int J Legal Med 107:283–290
Puers C, Hammond HA, Jin L, Caskey CT, Schumm JW (1993 a) Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTH01 [AATG]n and reassignment of alleles in population analysis by using a locus-specific allelic ladder. Am J Hum Genet 53:953–958
Puers C, Lins AM, Sprecher CJ, Brinkmann B, Schumm JW (1993 b) Analysis of polymorphic short tandem repeat loci using well-characterized allelic ladders. In: Proceedings from the fourth international symposium on human identification. Promega, Madison, USA, pp 161–172
Puers C, Hammond HA, Caskey CT, Lins AM, Sprecher CJ, Brinkmann B, Schumm JW (1994) Allelic ladder characterization of the short tandem repeat polymorphism located in the 5′ flanking region to the human coagulation factor XIII A subunit gene. Genomics 23:260–264
Sajantila A, Pacek P, Lukka M, Syäven AC, Nokelainen P, Sistonen P, Peltonen L, Budowle B (1994) A microsatellite polymorphism in the von Willebrand Factor gene: comparison of allele frequencies in different population samples and evaluation for forensic medicine. Forensic Sci Int 68:91–102
Sato K (1995) Medico-legal application of DNA short tandem repeat polymorphisms. Acta Crim Japon 61:34–51
Wiegand P, Budowle B, Rand S, Brinkmann B (1993) Forensic validation of the STR systems SE33 and TC11. Int J Legal Med 105:315–320
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Nagai, A., Yamada, S., Watanabe, Y. et al. Analysis of the STR loci HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 in a Japanese population. Int J Leg Med 109, 34–36 (1996). https://doi.org/10.1007/BF01369599
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DOI: https://doi.org/10.1007/BF01369599