Abstract
Allele and phenotype frequencies for two tetranucleotide STR (short tandem repeat or microsatellite) systems, HUMvWFA31/A and HUMF13A01, were obtained from a sample of approximately 240 unrelated individuals randomly selected from the French Caucasian population. PCR (polymerase chain reaction) products were analysed on 6% polyacrylamide denaturing gels and visualized using fluorescently labelled primers on the automated 373A ABI DNA sequencer (Applied Biosystems Inc.). French Caucasian allele frequencies were compared to other published Caucasian data. Conditions were optimised for the quadruplex PCR amplification of these two STR loci together with the HUMFESFPS and HUMTHOI loci and the quadruplex PCR was also performed on various forensic DNA samples.
Similar content being viewed by others
References
Buscemi L, Cucurachi N, Mencarelli R, Tagliabracci A, Wiegand P, Ferrara SD (1995) PCR analysis of the short tandem repeat (STR) system HUMVWA31. Allele and genotype frequencies in an Italian population sample. Int J Legal Med 107: 171–173
DNA recommendations-1994 report concerning further recommendations of the DNA commission of the International Society for Forensic Haemogenetics regarding PCR-based polymorphisms in STR (Short Tandem Repeat) systems. Int J Legal Med 107: 159–160
Drozd MA, Archard L, Lincoln PJ, Morling N, Nellemann LJ, Phillips C, Soteriou B, Syndercombe Court D (1994) An investigation of the HUMVWA31A locus in British Caucasians. Forensic Sci Int 69: 161–170
Dupuy BM, Berg ES, Olaisen B (1993) Four STRs in 300 Norwegians. In: Bär W, Fiori A, Rossi U (eds) Advances in forensic haemogenetics 5. Springer, Berlin Heidelberg New York, pp 539–541
Furedi S, Woller J, Padar Z (1995) Hungarian population data for the STR systems TH01 and VWA. Int J Legal Med 108: 48–49
Gill P, Evett I (1995) Population genetics of short tandem repeat (STR) loci. Genetica 96: 69–87
Hammond HA, Lin J, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of thirteen short tanden repeat loci for use in personal identification applications. Am J Hum Genet 55: 175–189
Hochmeister MN, Jung JM, Budowle B, Borer UV, Dirnhofer R (1994) Swiss population data on three tetrameric short tandem repeat loci-VWA, HUMTHOI, and F13A1-derived using multiplex PCR and laser fluorescence detection. Int J Legal Med 107: 34–36
Kimpton CP, Walton A, Gill P (1992) A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Genet 1: 287
Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods Appl 3: 13–22
Kimpton C, Gill P, D'Aloja E, Anderson JF, Bär W, Holgersson S, Jacobson S, Johnsson V, Kloosterman AD, Laren MV, Nellemann L, Pfitzinger H, Phillips, Rand S, Schmitter H, Schneider PM, Sternersen M, Wide MC (1995) Report on the second EDNAP collaborative exercise. Forensic Sci Int 71: 137–152
Lewomin RC, Felsenstein J (1965) The robustness of homogeneity tests in 2XN tables. Biometrics 21: 19–33
Lorente JA, Lorente M, Budowle B, Wilson MR, Villanueva E (1994) Analysis of short tandem repeat (STR) HUMVWA in the Spanish population. Forensic Sci Int 65: 169–175
Lygo JE, Johnson PE, Holdaway DJ, Woodroffe S, Whitaker JP, Clayton TM, Kimpton CP, Gill P (1994) The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Legal Med 107: 77–89
Möller A, Wiegand P, Grüschow C, Seuchter SA, Baur MP, Brinkmann B (1994) Population data and forensic efficiency values for the STR systems HUMVWA, HUMMBP, and HUMFABP. Int J Legal Med 106: 183–189
Pfitzinger H, Ludes B, Kintz P, Tracqui A, Mangin P (1995) French Caucasian population data for the HUMTHOI and HUMFESFPS short tandem repeat (STR) systems. J Forensic Sci 40:270–274
Polymeropoulos MH, Xiao H, Rath DS, Merril CR (1991a) Tetranucleotide repeat polymorphism at the Human Tyrosine Hydroxylase Gene (TH). Nucleic Acids Res 19: 3753
Polymeropoulos MH, Rath DS, Xiao H, Merril CR (1991b) Tetranucleotide repeat polymorphism at the Human c-fes/fps protooncogene (FES). Nucleic Acids Res 19: 4018
Polymeropoulos MH, Rath SD, Xiao H, Merril CR (1991c) Tetranucleotide repeat polymorphism at the human coagulation factor XIII A subunit gene (F13A1). Nucleic Acids Res 19: 4036
Puers C, Hammond HA, Jin L, Caskey CT, Schumm JW (1993) Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTH01 (AATG)n and reassignment of alleles in population analysis by using a locus-specific allelic ladder. Am J Hum Genet 53: 953–958
Robertson J, Ziegle J, Kronick M, Madden D, Budowle B (1991) Genetic typing using automated electrophoresis and fluorescence detection. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA fingerprinting: approaches and applications. Birkhauser, Basel, pp 391–398
Roff DA, Bentzen P (1989) The statistical analysis of mitochondrial DNA polymorphisms and the problem of small samples. Mol Biol Evol 6: 539–545
Sajantila A, Pacek P, Lukka M, Syvänen AC, Nokelainen P, Sistonen P, Peltonen L, Budowle B (1994) A microsatellite polymorphism in the von Willebrand factor gene: comparison of allele frequencies in different population samples and evaluation for forensic medicine. Forensic Sci hit 68: 91–102
Schwartz D (1963) Comparaison d'un pourcentage observé à un pourcentage théorique. Principe des tests (in French). In: Méthodes statistiques à l'usage des médecins et des biologistes. Collection statistique en biologie et en médecine, Flammarion Medecine Sciences 3ème édition, pp 37–44
Urquhart A, Oldroyd NJ, Kimpton CP, Gill P (1995) Highly discriminating heptaplex short tandem repeat PCR system for forensic identification. Biotechniques 18(1): 116–121
Whitaker JP, Clayton TM, Urquhart AJ, Millican ES, Downes TJ, Kimpton CP, Gill P (1995) Short tandem repeat typing of bodies from a mass disaster: high success rate and characteristic amplification patterns in highly degraded samples. Biotechniques 18: 670–677
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rousselet, F., Pfitzinger, H. & Mangin, P. French Caucasian population data obtained from fluorescently detected HumvWFA31/A and HumF13A01 short tandem repeat loci. Int J Leg Med 109, 5–9 (1996). https://doi.org/10.1007/BF01369594
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01369594