Abstract
The purpose of this study was to define the existence of an intestinal phase of pancreatic polypeptide (PP) release and to assess whether it was mediated by a cholinergic-sensitive mechanism. Four conscious dogs with 20-cm upper intestinal Thiry-Vella loops and chronic gastric fistulas were used. The Thiry-Vella (T-V) loops were perfused with 10% liver extract or 0.154 M NaCl at a rate of 1 ml/min for 120 min. In a separate experiment, 240 ml of 10% liver extract was infused over a 5-min period into the stomach via the gastric fistula. Basal PP levels were 29±4 fmol/ml. The gastric infusion of liver extract caused a significant increase of plasma PP levels to a peak of 215±29 fmol/ml (P<0.05). The perfusion of the T-V loop with liver extract significantly increased plasma PP levels over basal to a peak of 73±14 fmol/ml (P<0.05). This value was significantly less than that released by gastric infusion of liver extract (P<0.05). Perfusion of the loop with NaCl did not significantly alter basal plasma PP levels (P>0.05). PP release by perfusion of the T-V loop with liver extract was abolished by atropine intravenous bolus (0.2 mg/kg). Although the combination of bethanechol (100 μg/kg/hr intravenous) and liver extract consistently increased the plasma levels of PP, the values did not attain statistical significance when compared to liver extract alone (P>0.05). The data presented are thus consistent with the hypothesis that there is an enteric phase of pancreatic polypeptide release and that this enteropancreatic reflex is mediated by a cholinergic-sensitive mechanism which might be hormonal or neural.
Similar content being viewed by others
References
Kimmel JR, Hayden LJ, Pollock HG: Isolation and characterization of a new pancreatic polypeptide hormone. J Biol Chem 250:9369–9376, 1975
Gepts W, Baetens D, DeMay J: The PP Cell.In Gut Hormones, SR Bloom (ed). Edinburgh, Churchill Livingston, 1978, pp 229–233
Arimura A, Meyers CA, Case WL, Murphy WA, Schally AV: Suppression of somatostatin levels in the hepatic portal and systemic plasma of the rat by synthetic human pancreatic polypeptide. Biochem Biophys Res Commun 89(3):913–918, 1979
Greenberg GR, McCloy RF, Chadwick VS, Adrian TE, Baron JH, Bloom SR: Effect of bovine pancreatic polypeptide on basal pancreatic and biliary outputs in man. Dig Dis Sci 24:11, 1979
Lin TM, Chance RE: Spectrum of gastrointestinal actions of bovine PP.In Gut Hormones, SR Bloom (ed). Edinburg, Churchill Livingston, 1978, pp 242–246
Floyd JC Jr: Human pancreatic polypeptide. Clin Endocrinol Metab 8:379, 1979
Taylor IL, Feldman M, Richardson CT, Walsh JH: Gastric and cephalic stimulation of human pancreatic polypeptide release. Gastroenterology 75:432–437, 1978
Schwartz TW, Holst JJ, Fahrenkrug J, Lindkaer Jensen S, Nielsen OV, Rehfeld JF, Schaffalitzky de Muckadell ON, Stadil F: Vagal, cholinergic regulation of pancreatic polypeptide secretion. J Clin Invest 61:781–789, 1978
Modlin IM, Lamers CB, Jaffe BM: Evidence for cholinergic dependence of pancreatic polypeptide release by bombesin—a possible application. Surgery 88:75–85, 1980
Schwartz TW, Stenquist B, Olbe L: Physiology of mammalian PP and the importance of vagal regulation.In Gut Hormones, SR Bloom (ed). Edinburgh, Churchill Livingston, 1978, pp 261–264
Taylor IL, Kaufman G, Walsh JH, Grossman M: Release of pancreatic polypeptide by intestinally perfused liver extract. Gastroenterology 76:1259A, 1979
Owyang C, Krasman M, Vinik AI: The intestinal phase of human pancreatic polypeptide (hPP) release. Clin Res 27(4):634A, 1979
Modlin IM, Albert D, Crochelt R, Sank A, Jaffe BM: Evidence for a cholinergic stimulatory mechanism for pancreatic polypeptide release. Reg Peptides, Suppl (1):S76, 1980
Taylor IL, Impicciatore M, Carter DC, Walsh JH: Effect of atropine and vagotomy on pancreatic polypeptide response to a meal in dogs. Am J Physiol 235:E443–447, 1978
Stern AI, Hansky J, Korman M, Coopland G, Waugh J: Pancreatic polypeptide release following surgery for duodenal ulcer disease. Dig Dis Sci 25(7):485–488, 1980
Floyd JC, Fajans SS, Peck S: Physiologic regulation of plasma levels of PP in man.In Gut Hormones, SR Bloom (ed). Edinburgh, Churchill Livingston, 1978, pp 247–253
Author information
Authors and Affiliations
Additional information
This work was supported in part by USPHS grant 5-R01 AM265-22-02 and by the Foundation for Surgical Education and Investigation.
Rights and permissions
About this article
Cite this article
Modlin, I.M., Albert, D., Crochelt, R. et al. Evidence for an intestinal mechanism of pancreatic polypeptide release. Digest Dis Sci 26, 587–590 (1981). https://doi.org/10.1007/BF01367669
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01367669