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Regulation of exocytosis in electrically permeabilized insulin-secreting cells. Evidence for Ca2+ dependent and independent secretion

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Bioscience Reports

Abstract

The regulation of insulin secretion from RINm5F cells exposed to high voltage discharge has been investigated. Electron microscopy revealed that the overall structure of the cells was preserved after permeabilization. In this preparation insulin release was stimulated by Ca2+ (EC50=2.4 μM). The stable GTP analogue GTPγS enhanced secretion both at intermediate (nano- to micromolar) and vanishingly low (<10 pM) Ca2+ concentrations. At optimal Ca2+ (10 μM) the effect of GTPγS was greatly reduced. We investigated whether the secretory response to GTP analogues was mediated by any of three enzyme systems regulated by GTP-binding proteins, i.e. generation of cyclic AMP by adenylate cyclase, of diacylglycerol by phospholipase C and of arachidonic acid by phospholipase A2. The involvement of these messenger systems could be excluded as (i) cyclic AMP only had minor, Ca2+ dependent effects, (ii) phospholipase C was not activated in the absence of Ca2+ and insulin secretion due to the phorbol ester TPA displayed a different Ca2+ dependency, (iii) arachidonic acid did not elicit Ca2+ independent insulin secretion. These results, taken together with the finding that insulin secretion due to Ca2+ or TPA is attenuated by the inhibitory guanine nucleotide GDPβS, suggest the existence of a regulatory site in exocytosis which is sensitive to guanine nucleotides.

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Abbreviations

InsP3 :

inositol trisphosphate

Ptd-InsP2 :

phosphatidylinositol 4,5-bisphosphate

GTPγS:

guanosine 5′-(3-O-thio)triphosphate

GDPβS:

guanosine 5′-(2-O-thio)diphosphate

Gpp(NH)p:

guanyl-5′-yl imidodiphosphate

TPA:

12-O-tetradecanoylphorbol-13-acetate

OAG:

1-oleoyl-2-acetylglycerol

Hepes:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

EGTA:

(ethylenebis(oxyethylenenitrilo)tetraacetic acid

DAG:

diacylglycerol

[Ca2+]i :

cytosolic free Ca2+ concentration

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Authors and Affiliations

  1. Institut de Biochimie Clinique, Centre Médical Universitaire, University of Geneva, 1211, Geneva 4, Switzerland

    Claes B. Wollheim & Susanne Ullrich

  2. Institut d'Histologie et d'Embryologie, Centre Médical Universitaire, University of Geneva, 1211, Geneva 4, Switzerland

    Paolo Meda

  3. Department of Medical Pharmacology, University of Milano, Milano, Italy

    Lucia Vallar

Authors
  1. Claes B. Wollheim
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  2. Susanne Ullrich
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  3. Paolo Meda
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  4. Lucia Vallar
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Wollheim, C.B., Ullrich, S., Meda, P. et al. Regulation of exocytosis in electrically permeabilized insulin-secreting cells. Evidence for Ca2+ dependent and independent secretion. Biosci Rep 7, 443–454 (1987). https://doi.org/10.1007/BF01362507

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