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Interferon cures cells lytically and persistently infected with African swine fever virus in vitro

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Summary

Human interferon alpha (IFN-α) and interferon gamma (IFN-γ) inhibit African swine fever (ASF) virus replication in Vero cells. IFN-α and IFN-γ exert a synergistic inhibition. Human tumor necrosis factor (TNF) does not inhibit ASF virus replication in this cell line, but in combination with IFNs it has antiviral enhancing activity. Analysis of the mechanism of inhibition suggests that the action of these cytokines blocks a step that comes prior to DNA replication. The 2′-5′ A synthetase activity is induced in Vero cells by treatment with these cytokines and is activated after ASF virus infection. More interesting is the finding that continuous treatment with IFN-α cures Vero cells from lytic and persistent infections with ASF virus. A potential application of IFN for the treatment of animals carrying the virus is suggested.

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References

  1. Anderson KP, Fennie E (1987) Adenovirus early region 1A modulation of interferon antiviral activity. J Virol 61: 787–795

    Google Scholar 

  2. Cayley PJ, Davies J, McGullagh KG, Kerr IM (1984) Activation of the ppp(A2′p)nA system in interferon-treated, herpes simplex virus infected cells and evidence for novel inhibitors of the ppp(A2′p)nA-dependent RNase. Eur J Biochem 143: 165–174

    Google Scholar 

  3. Crespi M, Chiu MN, Struthers JK, Schoub BD, Lyons SF (1988) Effect of interferon on Vero cells persistently infected with Sendai virus compared to Vero cells persistently infected with SSPE virus. Arch Virol 98: 235–251

    Google Scholar 

  4. Enjuanes L., Carrascosa AL, Moreno MA, Viñuela E (1976) Titration of African swine fever (ASF) virus. J Gen Virol 32: 471–477

    Google Scholar 

  5. Esparza I, Gonzalez JC, Viñuela E (1988) Effect of interferon-α, interferon-γ and tumor necrosis factor on African swine fever virus replication in porcine monocytes and macrophages. J Gen Virol 69: 2973–2980

    Google Scholar 

  6. Feduchi E, Carrasco L (1987) Adenovirus infection reverses the antiviral state induced by human interferon. FEBS Lett 214: 153–157

    Google Scholar 

  7. Garcia-Barreno B, Sanz A, Nogal ML, Viñuela E, Enjuanes L (1986) Monoclonal antibodies of African swine fever virus. Antigenic differences among field virus isolates and viruses passaged in cell cultures. J Virol 58: 385–392

    Google Scholar 

  8. Gil-Fernandez C, Garcia-Villalon D (1988) A model virus-cell system to study the persistence of African swine fever virus. Arch Virol 100: 161–169

    Google Scholar 

  9. Hess WR (1981) African swine fever: a reassessment. Adv Vet Sci Comp Med 25: 349–356

    Google Scholar 

  10. Joklik WK (1985) Interferons. In: Field BN (ed) Virology. Raven, New York, pp 281–307

    Google Scholar 

  11. Kitajewski J, Schneider RJ, Safer B, Munemitsu SM, Samuel CE, Thimmappaya B, Shenk T (1986) Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF2-α kinase. Cell 45: 195–200

    Google Scholar 

  12. Lengyel P (1982) Biochemistry of interferons and their actions. Annu Rev Biochem 52: 252–282

    Google Scholar 

  13. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  14. Paez E, Esteban M (1984) Resistance of vaccinia virus to interferon is related to an interference phenomenon between the virus and the interferon system. Virology 134: 12–28

    Google Scholar 

  15. Paez E, Esteban M (1984) Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2′p)nA-synthetase. Virology 134: 29–39

    Google Scholar 

  16. Paez E, Esteban M (1985) Interferon prevents the generation of spontaneous deletions at the left terminus of vaccinia DNA. J Virol 56: 75–84

    Google Scholar 

  17. Rice AP, Roberts WK, Kerr IM (1984) 2′-5′ A accumulates to high levels in interferontreated, vaccinia virus-infected cells in the absence of any inhibition of virus replication. J Virol 50: 220–228

    Google Scholar 

  18. Salas J, Viñuela E (1986) Establishment of a Vero cell line persistently infected with African swine fever virus. J Virol 58: 676–679

    Google Scholar 

  19. Stewart WE II (1979) The interferon system. Springer, New York Berlin Heidelberg

    Google Scholar 

  20. Tabares E, Martinez J, Ruiz-Gonzalvo F, Sanchez-Botija C (1980) Proteins specified by african swine fever virus. II. Analysis of proteins in infected cells and antigenic properties. Arch Virol 66: 119–132

    Google Scholar 

  21. Viñuela E (1985) African swine fever virus. Curr Top Microbiol Immunol 116: 151–170

    Google Scholar 

  22. Viñuela E (1987) Molecular biology of african swine fever virus. In: Becker Y (ed) African swine fever. Martinus Nijhoff, The Hague, pp 31–49

    Google Scholar 

  23. Whitaker-Dowling P, Youngner J (1983) Vaccinia rescue of VSV from interferon-induced resistance: reversal of translation block and inhibition of protein kinase activity. Virology 131: 128–136

    Google Scholar 

  24. Wong GHW, Goeddel DV (1986) Tumor necrosis factors α and β inhibit virus replication and synergize with interferons. Nature 323: 819–822

    Google Scholar 

  25. Youngner J, Thacore H, Kelly M (1972) Sensitivity of ribonucleic acid and deoxyribonucleic acid viruses to different species of interferon in cell culture. J Virol 10: 171–181

    Google Scholar 

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Authors and Affiliations

  1. Department of Virology, Centro de Investigaciones Biologicas (CSIC), Madrid, Spain

    E. Paez, F. Garcia & Carmen Gil Fernandez

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  1. E. Paez
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  2. F. Garcia
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  3. Carmen Gil Fernandez
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Paez, E., Garcia, F. & Gil Fernandez, C. Interferon cures cells lytically and persistently infected with African swine fever virus in vitro. Archives of Virology 112, 115–127 (1990). https://doi.org/10.1007/BF01348989

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  • DOI: https://doi.org/10.1007/BF01348989

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