Summary
An exposure system for examining in vitro effects of microwave irradiation on cellular and subcellar components has been developed. The system was used to test the effect of 2.45-GHz CW microwaves on the release of two lysosomal enzymes. At a specific absorption rate (SAR) of 10, 50 or 100 mW/g (90 min) no effects were noted at 37° C (pH of 7.3) on lysosomal fragility as determined by the release of the lysosomal enzymes cathepsin D andβ-glucuronidase. When the medium was adjusted to pH 5.0, microwave irradiation of the lysosomal suspension had no effect on the acid-induced enhancement of release of lysosomal enzymes. The data indicate that microwave irradiation had no labilizing effect on the lysosomal membrane, although other microwave-membrane interactions not associated with enzyme release may occur.
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References
Allison, A. C., Mallucci, L.: Lysosomes in dividing cells with special reference to lymphocytes. Lancet1964 II, 1371–1373
Anson, M. L.: Estimation of cathepsin with hemoglobin and the partial purification of cathepsin. J. Gen. Physiol.20, 565–576 (1936)
Baranski, B.: Histological and histochemical effects of microwave irradiation on the central nervous system of rabbits and guinea pigs. Am. J. Phys. Med.51, 182–187 (1972)
Baranski, S., Czerski, P.: Biological effects of microwaves. p. 234. Pennsylvania: Dowden, Hutchinson and Ross Inc. 1976
Baranski, S., Szmigielski, S., Moneta, J.: In: Biological effects and health hazards of microwaves. Czerski, P. (ed.), p. 173. Warsaw, Poland: Polish Medical Publishers 1975
Chernovetz, M. E., Justesen, D. R., Oke, A. F.: A Teratologic Study of the Rat: microwave and infrared radiations compared. Radio Sci.12, 191–198 (1977)
Chou, C. K., Guy, A. W.: Effects of electromagnetic fields on isolated nerve and muscle preparations. IEEE Trans, Microwave Theory Tech.26, 141–147 (1978)
Cleary, S. F.: Biological effects of microwave and radiofrequency radiation. CRC Crit. Rev. Environ. Control7, 121–166 (1977)
Elder, J. A., Ali, J. S.: The effect of microwave (2450 MHz) on isolated rat liver mitochondria. Ann. N. Y. Acad. Sci.247, 251–259 (1975)
Ferguson, W. W., Glenn, T. M., Lefer, A. M.: Mechanisms of production of circulatory shock factors in isolated perfused pancreas. Am. J. Physiol.222, 450–457 (1972)
Gordis, L.: Lysosomes and Disease. J. Pediatr.68, 638–649 (1966)
Gornall, A. G., Bardowill, C. J., and David, M. M.: Determination of serum proteins by means of the biuret reaction. J. Biol. Chem.177, 751–766 (1949)
Guth, P. S., Amaro, J., Sellinger, O. Z. and Elmer, L.: Studies in vitro and in vivo of the effects of chlorpromazine on rat liver lysosomes. Biochem. Pharm.14, 769–775 (1965)
Johnson, C. C. and Guy, A. W.: Nonionizing electromagnetic wave effects on biological material and systems. Proc. IEEE60, 692–718 (1972)
Kramer, P., Harris, C., Emery, A., and Guy, A.: Acute microwave irradiation and cataract formation in rabbits and monkeys. J. Microwave Power13, 239–249 (1978)
Livingston, G. K., Johnson, C. C. and Dethlefsen, L. A.: Comparative effects of water-bath and microwave induced hyperthermia on survival of Chinese hamster ovary, (CHO) cells. Radio Sci.14, 117–123 (1979)
Mayers, C. P., and Habeshaw, J. A.: Depression of phagocytosis: a non-thermal effect of microwave radiation as a potential hazard to health. Int. J. Radiat. Biol.24, 449–461 (1973)
McRee, D. I.: Determination of the absorption of microwave radiation by a biological specimen in a 2450 MHz microwave field. Health Phys26, 385–390 (1974)
Peters, W. J., Jackson, R. W., and Iwano, K.: Effect of controlled electromagnetic radiation on the growth of cells in tissue culture. J. Surg. Res.27, 8–13 (1979)
Sawicki, W. and Ostrowski, K.: Non-thermal effect of microwave radiation in vitro on peritoneal mast cells of the rat. Am. J. Physical Med.17, 225–232 (1968)
Snyder, S. L.: Radiation-induced alterations in splenic and lysosomal hydrolases of rat. Radiat. Res.69, 306–316 (1977)
Smurova, E. I.: Changes in the phagocytic and bactericidal functions of blood in animals exposed to electromagnetic field at radio frequencies. G. Saint (Health and Sanitation)32, 359–363 (1967)
Szmegielski, S., Feljas, J. and Wiranowska, M.: Acute Staphyloccal infections in rabbits irradiated with 3-GHz microwaves. Ann. N. Y. Acad. Sci.247, 305–312 (1975)
Szmegielski, S., Luczak, M., and Weranowska, M.: Effect of microwaves on cell function and virus replication in cell cultures irradiated in vitro. Ann. N. Y. Acad. Sci.247, 263–271 (1975)
Talalay, P., Fishman, W. H., and Huggins, C. J.: Chromogenic substances I: Phenolphthalein glucuronic acid as substrate for the assay of glucuronidase activity. J. Biol. Chem.166, 757–772 (1946)
Thomas, J. R., Burch, L. S., and Teandle, S. S.: Microwave radiation and chlordiazepoxide: Synergistic effects on fixed internal behavior. Science203, 1357–1358 (1979)
Varma, M. M. and Traboulay, Jr., E. A.: Biological effects of microwave radiation on the testes of swiss mice. Experientia31, 301–302 (1975)
Weissman, G.: Lysosomes. New Engl. J. Med.273, 1084–1090, 1143–1149 (1965)
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Galvin, M.J., Parks, D.L. & McRee, D.I. Microwave irradiation and in vitro release of enzymes from hepatic lysosomes. Radiat Environ Biophys 18, 129–136 (1980). https://doi.org/10.1007/BF01326051
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DOI: https://doi.org/10.1007/BF01326051