Summary
In the WAG/Rij nude rat, subcutaneous (s.c.) and intracerebral (i.e.) xenografts of the human SCLC cell line GLC-28 were evaluated for their growth behavior,in vivo monoclonal antibody binding and presence of tumor infiltrating lymphocytes. For the i.e. xenografts, two models of cerebral tumor growth were studied, one in the cerebral cortex and one in the lateral ventricle of the brain. In the s.c. and both i.c. xenografts models,in vivo localization of anti-carcinoma moab MOC-31 occurred within 4 hours after i.p. injection, with a maximal binding at 24 h after injection. A pronounced tumor infiltration of predominantly NK cells was observed for s.c. and intraventricular xenografts, but not for the GLC-28 tumors xenografted in the cerebral cortex. The presented nude rat/GLC-28 xenograft models may be used for thein vivo testing of experimental imaging techniques or alternative treatment strategies relevant to brain metastases of human SCLC.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hirsch FR, Osterlind K, Hansen HH: The prognostic significance of histopathologic subtyping of small cell-carcinoma of the lung according to the classification of the World Health Organization — A study of 375 consecutive patients. Cancer 52: 2144–2150, 1983
Sheenan RG: Southwestern internal medicine conference: Small cell lung cancer: A Problem of tumor heterogeneity. Am J Med Sci 292: 241–256, 1986
Olsson L, Sorensen HR, Behnke O: Intratumoral phenotypic diversity of cloned human lung tumor cell lines and consequences for analyses with monoclonal antibodies. Cancer 54: 1757–1765, 1984
Fargion S, Carney D, Mulshine J, Rosen S, Bunn P, Jewett P, Cuttitta F, Gazdar A, Minna J: Heterogeneity of cell-surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. Cancer Res 46: 2633–2638, 1986
Souhami RL, Beverley PCL, Bobrow LG: Antigens of small cell lung cancer — 1st international workshop. Lancer 1987, 2: 325–326
Ihde DC: Current status of therapy for small cell carcinoma of the lung. Cancer 54: 2722–2728, 1984
Nugent JL, Bunn PA, Matthews MJ, Ihde DC, Cohen MH, Gazdar A, Minna JD: CNS metastases in small cell bronchogenic carcinoma. Increasing frequency and changing pattern with lengthening survival. Cancer 44: 1885–1893, 1979
Hirsch FR, Paulson OB, Hansen HH, Larsen SO: Intracranial metastases in small cell carcinoma of the lung. Cancer 51: 529–533, 1983
van Hazel GA, Scott M, Eagan RT: The effect of CNS metastases on the survival of patients with small cell cancer of the lung. Cancer 51: 933–937, 1983
Hirsch FR, Paulson OB, Hansen HH, Vraa-Jensen H: Intracranial metastases in small cell carcinoma of the lung. Correlation of clinical and autopsy findings. Cancer 50: 2433–2437, 1982
Pedersen AG, Karle A, Boysen G, Hojgaard K, Dombernovsky P: Brain CT-scanning and neurological examination in small cell bronchogenic carcinoma. J Neur Oncol 1: 197–202, 1983
Flanagan SP: ‘Nude’, a new hairless gene with pleiotropic effects in the mouse. Genet Res 8: 295–309, 1966
Pantelouris EM: Absence of thymus in a mouse mutant. Nature 217: 370–371, 1968
Festing MFW, May D, Connors TA, Lovell D, Sparrow S: An athymic nude mutation in the rat. Nature 274: 365–366, 1978
Stromblad LG, Brun A, Salford LG, Stenevi U: A model for xenotransplantation of human malignant astrocytomas into the brain of normal adult rats. Acta Neurochir 65: 217–226, 1982
Saris SC, Bigner SH, Bigner DD: Intracerebral transplantation of a human gliome line in immunosuppressed rats. J Neurosurg 60: 582–588, 1984
Neuwelt EA, Frenkel EP, D'Agostino AN, Carney DN, Minna JD, Barnett PA, McCormick CI: Growth of human lung tumor in the brain of the nude rat as a model to evaluate antitumor agent delivery across the blood-brain-barrier. Cancer Res 45: 2827–2833, 1985
de Leij L, Postmus PE, Buys CHCM, Elema JD, Ramaekers F, Poppema S, Brouwer M, van der Veen AY, Mesander G, The TH: Characterization of three new variant type cell lines derived from small cell carcinoma of the lung. Cancer Res 45: 6024–6033, 1985
de Leij L, Berendsen H, Spakman H, ter Haar A, The TH: Neuroendocrine and epithelial antigens in SCLC. Lung Cancer 4: 42–44, 1988
Jefferies WA, Green JR, Williams AF: Authentic T-helper CD4-(W3/25) antigen on rat peritoneal macrophages. J Exp Med 165: 117–127, 1985
Williams AF, Barclay AN, Clark SJ, Paterson DJ, Willis AC: Similarities in sequences and cellular expression between rat CD2 and CD4 antigens. J Exp Med 165: 368–380, 1987
Kasai M, Iwamori M, Nagai Y, Okamura K, Tada T: A glycolipid on the surface of mouse natural killer cells. Eur J Immunol 10: 175–180, 1980
de Jonge MWA, Kosterink JGW, Yu Yb, Bulte JWM, Kengen RAM, Piers DB, The TH, de Leij L: Radioimmunodetection of human small cell lung carcinoma xenografts in the nude rat using111In-labeled monoclonal antibody MOC-31. Eur J Cancer Clin Oncol, in press.
Giovanella BC, Fogh J: Present and future trends in investigations with the nude mouse as a recipient of human tumor transplants. In: Fogh J, Giovanella BC (eds) The nude mouse in experimental and clinical research. Academic Press, New York, 1978, pp 281–312
Janzer RC, Raff MC: Astrocytes induce blood-brain-barrier properties in endothelial cells. Nature 325: 253–257, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bulte, J.W.M., Go, K.G., Zuiderveen, F. et al. Intracerebral and subcutaneous xenografts of human SCLC in the nude rat: comparison of monoclonal antibody localization and tumor infiltrating lymphocytes. J Neuro-Oncol 16, 11–18 (1993). https://doi.org/10.1007/BF01324829
Issue Date:
DOI: https://doi.org/10.1007/BF01324829