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The effect of organic calcium channel modulators on the germination ofVaucheria longicaulis aplanospores

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Summary

InVaucheria longicaulis var.macounii aplanospore germination and filament growth are severely inhibited by the Ca2+-channel antagonists (−)202–791, diltiazem, nifedipine and verapamil, whereas the agonists (+)202–791, Bay K-8644 and CGP-28392 stimulate those processes. Both antagonist and agonist actions suggest that voltage-controlled Ca2+ influx plays a major role in the regulation of the initial events of germination and filament growth. Increases in45Ca2+ influx are observed after pretreatment of the aplanospores with low temperature shocks of brief duration or FCCP. Both agents are known to depolarize the surface membrane.45Ca2+ influx is reduced in material treated with FC, an agent known to hyperpolarize cell membranes. The results indicate that Ca2+ influx takes place through voltage-sensitive Ca-channels.

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Abbreviations

Bay K-8644:

methyl 1,4-dihydro-2,6-dimethy1-3-nitro-4-(2-trifluoromethylphenyl)-pyridine-5-carboxylate

CGP:

CGP-28392

CTC:

chlorotetracycline

dil:

diltiazem

DMSO:

dimethyl sulphoxide

DTE:

dithioerythritol

EGTA:

ethyleneglycol-bis-(β-aminoethylether) N,N1-tetraacetic acid

FC:

fusicoccin

FCCP:

p-fluoromethoxy-(carbonylcyanide)phenylhydrazone

nif:

nifedipine

PCMB:

p-chloromercuribenzoate

ver:

verapamil

References

  • Affolter H, Coronado R (1985) Agonists Bay K-8644 and CGP-28392 open calcium channels reconstituted from skeletal muscle transverse tubules. Biophys J 48: 341–347

    Google Scholar 

  • Andrejauskas E, Hertel R, Marme D (1985) Specific binding of the calcium antagonist [3H] verapamil to membrane fractions from plants. J Biol Chem 260: 5411–5414

    Google Scholar 

  • Bellemann P (1984) Interaction of the calcium channel activating3H-Bay K-8644 and inhibiting3H-verapamil with specific receptor sites on cultured beating myocardial cells. J Recept Res 4: 571–585

    Google Scholar 

  • —, Franckowiack G (1985) Different receptor affinities of the enantiomers of Bay K-8644, a dihydropyridine Ca channel activator. Eur J Pharmacol 118: 187–188

    Google Scholar 

  • Blackmore PF, El-Refai MF, Exton JH (1979) Adrenergic blockade and inhibition of A 23187 mediated Ca2+ uptake by the calcium antagonist verapamil in rat liver cells. Mol Pharmacol 15: 598–606

    Google Scholar 

  • Brownlee C, Pulsford AL (1988) Visualization of the cytoplasmic Ca2+ gradient inFucus serratus rhizoids: correlation with cell structure and polarity. J Cell Sci 91: 249–256

    Google Scholar 

  • —, Wood JW (1986) A gradient of cytoplasmic free calcium in growing rhizoid cells ofFucus serratus. Nature 320: 624–626

    Google Scholar 

  • Chen T-L, Wolniak SM (1987) Mitotic progression in stamen hair cells ofTradescantia is accelerated by treatment with ruthenium red and Bay K-8644. Eur J Cell Biol 45: 16–22

    Google Scholar 

  • Conrad PA, Hepler PK (1988) The effect of 1,4-dihydropyridines on the initiation and development of gametophore buds in the mossFunaria. Plant Physiol 86: 684–687

    Google Scholar 

  • Cunninghame ME, Hall JL (1986) The effect of calcium antagonists and inhibitors of secretory processes on auxin-induced elongation and fine structure ofPisum sativum stem segments. Protoplasma 133: 149–159

    Google Scholar 

  • De Quintero MR, Hanson JB (1984) Reactions of corn root tissue to calcium. Plant Physiol 76: 403–408

    Google Scholar 

  • Deitmer JW (1987) Multiple types of calcium channels: is their function related to their localization? In: Grinnel AD, David A, Jackson MB (eds) Calcium and ion channel modulation. Plenum, New York, pp 19–32

    Google Scholar 

  • Dolle R (1988) Isolation of plasma membrane and binding of the Ca2+ antagonist nimodipine inChlamydomonas reinhardtii. Physiol Plant 73: 7–14

    Google Scholar 

  • Fitch RS, Oliveira L (1986) Ultrastructure of aplanosporogenesis in the brackish-water algaVaucheria longicaulis var.macounii Blum (Tribophyceae). Bot Mar 29: 105–115

    Google Scholar 

  • Franckowiak G, Bechem M, Schramm M, Thomas G (1985) The optical isomers of the 1,4-dihydropyridine BAY K-8644 show opposite effects on Ca-channels. Eur J Pharmacol 114: 223–226

    Google Scholar 

  • Garbary DJ, Fitch RS (1984) Some brackish species ofVaucheria (Tribophyceae) from British Columbia and northern Washington. Nat Canad 111: 125–130

    Google Scholar 

  • Good N, Izawa S (1966) Uncoupling and energy transfer inhibition in photophosphorylation. Curr Top Bioenerg 1: 75–112

    Google Scholar 

  • Graziana A, Fosset M, Ranjera R, Hetherington AM, Lazdunski M (1987) Ca2+-channel blockers that bind plant cell membranes block Ca2+ entry into protoplasts. Biochemistry 27: 764–768

    Google Scholar 

  • Harrison PJ, Waters RE, Taylor FJR (1980) A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J Phycol 16: 28–35

    Google Scholar 

  • Hess P, Lansman JB, Tsien RY (1984) Different modes of Ca-channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature 311: 538–544

    Google Scholar 

  • Hetherington AM, Trewavas AJ (1984) Binding of nitrendipine, a calcium channel blocker, to pea shoot membranes. Plant Sci Lett 35: 109–113

    Google Scholar 

  • Hof RP, Ruegg UT, Hof A, Vogel A (1985) Stereoselectivity at the calcium channel: opposite action of the enantiomers of a 1,4-dihydropyridine. J Cardiovasc Pharmacol 7: 689–693

    Google Scholar 

  • Kropf DL, Quatrano RS (1987) Localization of membrane-associated calcium during development of fucoid algae using chlorotetracycline. Planta 171: 158–170

    Google Scholar 

  • Lin W, Hanson JB (1976) Cell potentials, cell resistance, and proton fluxes in corn root tissue. Effects of dithioerythritol. Plant Physiol 58: 276–282

    Google Scholar 

  • Lonergan TA, Williamson LC (1988) Regulation of cell shape inEuglena gracilis. V. Time-dependent responses to Ca2+ agonists and antagonists. J Cell Sci 89: 365–371

    Google Scholar 

  • Loutzenhiser R, Ruegg UT, Hof A, Hof RP (1984) Studies on the mechanism of action of the vasoconstrictive dihydropyridine, CGP 28392. Eur J Pharmacol 105: 229–237

    Google Scholar 

  • Marre E (1979) Fusicoccin: a tool in plant physiology. Annu Rev Plant Physiol 30: 273–288

    Google Scholar 

  • Muff R, Nemeth EF, Haller-Brem S, Fischer JA (1988) Regulation of hormone secretion and cytosolic Ca2+ in parathyroid cells and C-cells: role of voltage-sensitive Ca2+-channels. Arch Biochem Biophys 265: 128–135

    Google Scholar 

  • Nelles A, Laske E (1982) Temperature dependence of the membrane potential of corn coleoptile cells. Biochem Physiol Pflanzen 177: 107–113

    Google Scholar 

  • Nobiling R, Reiss H-D (1987) Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum. Protoplasma 139: 20–24

    Google Scholar 

  • Nuccitelli R (1978) Ooplasmic segregation and secretion in thePelvetia egg is accompanied by a membrane-generated electrical current. Dev Biol 62: 13–33

    Google Scholar 

  • Oliveira L, Fitch RS (1988 a) Morphometric analysis of cytological changes occurring inVaucheria longicaulis var.macounii Blum (Tribophyceae) during aplanospore germination. J Submicrosc Cytol Pathol 20: 397–406

    Google Scholar 

  • — — (1988 b) Visualization of Ca2+-gradients in germinating aplanospores ofVaucheria longicaulis var.macounii Blum (Tribophyceae) with chlorotetracycline fluorescence. J Submicrosc Cytol Pathol 20: 407–414

    Google Scholar 

  • — — (1988 c) The effects of Ca2+-modulation on the germination ofVaucheria longicaulis var.macounii aplanospores. J Submicrosc Cytol Pathol 20: 741–750

    Google Scholar 

  • Reiss H-D, Herth W (1985) Nifedipine-sensitive calcium channels are involved in polar growth of lily pollen tubes. J Cell Sci 76: 247–254

    Google Scholar 

  • Robinson KR, Jaffe LF (1975) Polarizing fucoid eggs drive a calcium current through themselves. Science 187: 70–72

    Google Scholar 

  • Reynolds IJ, Snyder SH (1988) Calcium antagonist receptors. In: Narahashi T (ed) Ion channels, vol 1. Plenum Press, New York, pp 213–249

    Google Scholar 

  • Rincon M, Hanson JB (1986) Controls on calcium ion fluxes in injured or shocked corn root cells: importance of proton pumping and cell membrane potential. Physiol Plant 67: 576–583

    Google Scholar 

  • Schnepf E (1986) Cellular polarity. Annu Rev Plant Physiol 37: 23–47

    Google Scholar 

  • Snedecor GW, Cochran WG (1967) Statistical methods. Iowa State University Press, Ames, Iowa

    Google Scholar 

  • Spedding M, Berg C (1984) Interaction between a “calcium channel agonist”, Bay K-8644, and calcium antagonists differentiate calcium antagonist subgroups in K+-depolarized smooth muscle. Naunyn Schmiedebergs Arch Pharmacol 328: 69–75

    Google Scholar 

  • Su CM, Swamy VC, Triggle DJ (1984) Calcium channel activation in vascular smooth muscle by Bay K-8644. Canad J Physiol Pharmacol 62: 1401–1410

    Google Scholar 

  • Varecka L, Peterajova E, Pogady J (1986) Inhibition by divalent cations and sulphydryl reagents of the passive Ca2+ transport in human red blood cells observed in the presence of vanadate. Biochim Biophys Acta 856: 585–594

    Google Scholar 

  • Zocchi G, Hanson JB (1982) Calcium influx into corn root cells as a result of cold shock. Plant Physiol 70: 318–319

    Google Scholar 

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  1. Department of Botany, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada

    L. Oliveira

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Oliveira, L. The effect of organic calcium channel modulators on the germination ofVaucheria longicaulis aplanospores. Protoplasma 158, 182–190 (1990). https://doi.org/10.1007/BF01323131

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  • DOI: https://doi.org/10.1007/BF01323131

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