Skip to main content
Log in

Polyacrylamide gel electrophoresis of avian rotavirus RNA

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 × 106 to 2.09 × 106. In contrast to calf rotavirus, segments 10 and 11 of avian rotavirus RNA had very similar or identical electrophoretic mobilities. This may be a unique feature of avian rotavirus RNA.

Co-electrophoresis experiments showed that the RNAs of 2 of the turkey isolates were electrophoretically identical. However, differences existed between these 2 isolates, the chicken isolate and the remaining turkey isolate with respect to segments 5, 6, the 7, 8, 9 complex and 10. Segment 5 of the chicken isolate was sometimes resolved into 2 components, and was further anomalous in that it was absent in some (analyses).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bergeland, M. E., McAdaragh, J. P., Stotz, I.: Rotaviral enteritis in turkey poults. Proc. 26th Western Poultry Disease Conference, University of California, Davis, 129–130 (1977).

  2. Flewett, T. H., Woode, G. N.: The rotaviruses. Arch. Virol.57, 1–23 (1978).

    Google Scholar 

  3. Jones, R. C., Hughes, C. S., Henry, R. R.: Rotavirus infection in commercial laying hens. Vet. Rec.104, 22 (1979).

    Google Scholar 

  4. Kalica, A. R., Sereno, M. M., Wyatt, R. G., Mebus, C. A., Chanock, R. M., Kapikian, A. Z.: Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology87, 247–255 (1978).

    Google Scholar 

  5. Kalica, A. R., Wyatt, R. G., Kapikian, A. Z.: Detection of differences among human and animal rotaviruses using analysis of viral RNA. J. Amer. Vet. Med. Assoc.173, 531–537 (1978).

    Google Scholar 

  6. McNulty, M. S.: Rotaviruses. J. gen. Virol.40, 1–18 (1978).

    Google Scholar 

  7. McNulty, M. S., Allan, G. M., McFerran, J. B.: Cell culture studies with a cytopathic bovine rotavirus. Arch. Virol.54, 201–209 (1977).

    Google Scholar 

  8. McNulty, M. S., Allan, G. M., Stuart, J. C.: Rotavirus infection in avian species. Vet. Rec.103, 319–320 (1978).

    Google Scholar 

  9. McNulty, M. S., Allan, G. M., Todd, D., McFerran, J. B.: Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Arch. Virol.61, 13–21 (1979).

    Google Scholar 

  10. McNulty, M. S., Gowans, E. J., Houston, M. J., Fraser, G.: Neuraminidase content of strains of Newcastle disease virus which differ in virulence. J. gen. Virol.27, 399–402 (1975).

    Google Scholar 

  11. Rodger, S. M., Holmes, I. H.: Comparison of the genomes of simian, bovine and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J. Virol.30, 839–849 (1979).

    Google Scholar 

  12. Todd, D., McNulty, M. S.: Characterization of pig rotavirus RNA. J. gen. Virol.33, 147–150 (1976).

    Google Scholar 

  13. Todd, D., McNulty, M. S.: Biochemical studies on a reovirus-like agent (rotavirus) from lambs. J. Virol.21, 1215–1218 (1977).

    Google Scholar 

  14. Verly, E., Cohen, J.: Demonstration of size variation of RNA segments between different isolates of calf rotavirus. J. gen. Virol.35, 583–586 (1977).

    Google Scholar 

  15. Wood, H. A.: Viruses with double-stranded RNA genomes. J. gen. Virol.20, 61–85 (1973).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

With 4 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Todd, D., McNulty, M.S. & Allan, G.M. Polyacrylamide gel electrophoresis of avian rotavirus RNA. Archives of Virology 63, 87–97 (1980). https://doi.org/10.1007/BF01320765

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01320765

Keywords

Navigation