Summary
Fetuin bound latex spheres do not adhere to the membranes of non-infected cells but adhere to those of cells productively infected by fowl plague virus (FPV Dobson strain). In contrast, asialo fetuin spheres do not attach to the membranes of productively infected cells. Moreover latex fetuin spheres incubated with extracts of productively infected cells and extensively washed are specifically enriched in neuraminidase activity without any trace of haemagglutinin. These observations suggest that viral neuraminidase in the membrane is the site of attachment of the sialic acid moieties of fetuin spheres. These neuraminidase sites are detectable when L cells are productively infected by a mammalian cell adapted mutant of the Dobson strain (FPV-B) but are not detectable on L cells abortively infected by wild type (FPV+). However, even in the abortive system, neuraminidase is synthesisedde novo as shown by its labelling with14C-glucosamine and by its isolation from labelled extracts of infected cells by latex fetuin spheres. These results show that misintegration of viral neuraminidase in the plasma membrane of L cells is a feature of abortive infection of these cells by the Dobson strain of FPV. However the relationship (if any) of this misintegration to abortive infection remains to be established.
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Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C., Webster, R. G.: Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bull. Wld. Hlth. Org.48, 199–202 (1973).
Becht, H., Rott, R.: Purification of influenza virus hemagglutinin by affinity chromatography. Med. Microbiol. Immunol.158, 67–70 (1972).
Bosch, F. X., Hay, A. J., Skehel, J. J.: RNA and protein synthesis in a permissive and an abortive influenza virus infection. In:Mahy, B. W. J., Barry, R. D. (eds.), Negative Strand Viruses and the Host Cell, pp. 465–474. London-New York-San Francisco: Academic Press 1978.
Bucher, D. J.: Chromatographic isolation of the major polypeptides of influenza virus. In:Mahy, B. W. J., Barry, R. D. (eds.), Negative Strand Viruses, pp. 133 to 143. London-New York-San Francisco: Academic Press 1975.
Carney, D. H., Cunningham, D.: Cell surface action of thrombin is sufficient to initiate division of chick cells. Cell14, 811–824 (1978).
Cuatrecasas, P., Afinsen, C. B.: Affinity chromatography. Ann. Rev. Biochem.40, 259–278 (1971).
Franklin, R. M., Breitenfeld, P. M.: Abortive infection of L cells by fowl plague virus. Virology8, 293–307 (1959).
Fraser, K. B.: Immunofluorescence of abortive and complete infection by influenza A virus in hamster BHK21 cells and mouse L cells. J. gen. Virol.1, 1–12 (1967).
Hay, A. J.: Studies on the formation of the influenza virus envelope. Virology60, 398–418 (1974).
Heatley, S. A., Gordon, I. L., O'Brien, R. L., Rembaum, A., Parker, J. W.: Lymphocyte transformation initiated by galactose oxidase coupled to latex microspheres and formalinized erythrocytes. Exp. Cell Res.108, 139–149 (1977).
Israël, A., Semmel, M., Huppert, J.: Host range mutant of fowl plague virus (FPV): comparison of the genome and virus proteins. Virology68, 503–509 (1975).
Kessler, S. W.: Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J. Immunol.11, 1617–1624 (1975).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–685 (1970).
Laskey, R. A., Mills, A. D.: Quantitative film detection of3H and14C in poly-acrylamide gels by fluorography. Europ. J. Biochem.56, 335–341 (1975).
Lohmeyer, J., Talens, L. T., Klenk, H. D.: Biosynthesis of the influenza virus envelope in abortive infection. J. gen. Virol.42, 73–88 (1979).
Mackenzie, J. S., Dimmock, N. J.: A preliminary study of physiological characteristics ofts mutants of influenza virus. J. gen. Virol.19, 51–63 (1973).
Quash, G. A., Niveleau, A., Aupoix, M., Greenland, T.: Immunolatex visualisation of cell surface Forssman and polyamine antigens. Exp. Cell Res.98, 253–261 (1976).
Quash, G. A., Roch, A. M., Niveleau, A., Grange, J., Keolouangkhot, T., Huppert, J.: The preparation of latex particles with covalently bound polyamines, IgG and measles agglutinins and their use in visual agglutination tests. J. immunol. Meth.22, 165–174 (1978).
Roch, A. M., Quash, G. A., Huppert, J.: Mise en évidence dans les sérums humains d'anticorps (IgG) réagissant spécifiquement avec les polyamines. C.R. Acad. Sci.287, 1071–1074 (1978).
Schulze, I. T.: The biologically active proteins of influenza virus: the hemagglutinin. In:Kilbourne E. D. (ed.), The influenza viruses and influenza, pp. 53–82. New York-San Francisco-London: Academic Press 1975.
Stanley, P., Crook, N. E., Streader, L. G., Davidson, B. E.: The polypeptides of influenza virus. VIII: Large scale purification of the haemagglutinin. Virology56, 640–645 (1973).
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Israël, A., Niveleau, A., Quash, G. et al. Latex fetuin spheres as probes for influenza virus neuraminidase in productively and abortively infected cells. Archives of Virology 61, 183–199 (1979). https://doi.org/10.1007/BF01318053
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DOI: https://doi.org/10.1007/BF01318053