Summary
The protein pattern of a type-D retrovirus (PMFV) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Staining with Coomassie blue and labeling with14C-leucine/14C-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. The 68,000D-protein was shown to be a glycoprotein by incorporation of3H-glucosamine and the 15,000D-protein was identified as a phosphoprotein.
By comparing PMFV with the closely related Mason-Pfizer monkey virus (MPMV) in co-electrophoresis experiments no clear difference was detected in viral14C-leucine/14C-lysine profiles. The viruses differ, however, with respect to their glycoprotein patterns. A glycoprotein corresponding to the gp20 of MPMV has not been detected in PMFV irrespective of the cell line used for propagation of viruses.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bauer, H., Daams, J. H., Watson, K. F., Mölling, K., Gelderblom, H., Schäfer, W.: Oncornavirus-like particles in HeLa cells. II. Immunological characterization of the virus. Intern. J. Cancer13, 254–261 (1974).
Bierwolf, D., Wunderlich, V., Micheel, B., Graffi, A.: Biological and biochemical properties of a human oncornavirus system (PMF). IN:Davis, W., Harrap, K. R. (eds.), Characterization and Treatment of Human Tumours. Adv. Tumor Prev., Detect. and Characterization4, 38–47 (1978).
Bolognesi, D. P., Bauer, H.: Polypeptides of avian RNA tumor viruses. I. Isolation and physical and chemical analysis. Virology42, 1097–1112 (1970).
Bryant, M. L., Sherr, C. L., Sen, A., Todaro, G. J.: Molecular diversity among five different endogenous primate retroviruses. J. Virol.28, 300–313 (1978).
Fine, D., Schochetman, G.: Type-D primate retroviruses: A review. Cancer Res.38, 3123–3139 (1978).
Graffi, A., Bierwolf, D., Widmaier, R., Bender, E., Wunderlich, V., Rudolph, M., Mothes, E., Niezabitowski, A., Papsdorf, G.: In der Gewebekultur züchtbares Oncorna-Virus in malignen permanenten menschlichen Zelllinien vom Embryo einer krebskranken Frau. Dtsch. Ges.wesen29, 1489–1498 (1974).
Iljin, K. V., Bykovsky, A. F., Zhdanov, V. M.: An oncornavirus isolated from human cancer cell line. Cancer37, 89–96 (1973).
Jamjoon, G. A., Ng, V. L., Arlinghaus, R. B.: Inhibition of maturation of RLV by amino acid analogous. J. Virol.25, 408–412 (1978).
Jensen, E. M., Zelljadt, I., Chopra, H. C., Mason, M. M.: Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey. Cancer Res.30, 2388–2393 (1970).
Lipp, M., Brandner, G., Bredt, W.: Simulation and prevention of oncovirus-specific reactions in the presence of mycoplasms. 11th meeting of the European Tumour Virus Group, Balatonfüred, Hungary, Abstracts, p. 219 (1978).
Maizel, J. V.: Acrylamide gel electrophoresis of proteins and nucleic acids: In:Habel, K., Salzman, N. P. (eds.), Fundamental techniques in Virology, 334–362. New York: Academic Press 1969.
Micheel, B., Papsdorf, G., Wunderlich, V., Niezabitowski, A., Widmaier, R., Bender, E., Graffi, A.: Serological analysis of an oncornavirus (PMF virus) detected in malignant permanent human cell lines. Acta biol. med. germ.34, K39-K45 (1975).
Micheel, B., Baumbach, L., Wunderlich, V., Niezabitowski, A., Bierwolf, D.: Serological evidence for antigenic differences between the Mason-Pfizer monkey virus (MPMV) and an MPMV-like virus (PMFV) detected in a malignant permanent human cell line. Europ. J. Cancer15, 101–108 (1979).
Micheel, B., Fiebach, H., Wunderlich, V.: Interspecies antigenic determinants of structural proteins of mammalian type-C viruses as detected by a competitive Sepharose bead immunofluorescence assay. Arch. Geschwulstforsch.49, 485–489 (1979).
Parks, W. P., Gilden, R. V., Bykovsky, A. F., Miller, G. G., Zhdanov, V. M., Soloviev, V. D., Scolnick, E. M.: Mason-Pfizer virus characterization: A similar virus in a human amniotic cell line. J. Virol.12, 1540–1547 (1973).
Schochetman, G., Fine, D., Arthur, L., Gilden, R., Heberling, R.: Characterization of a retravirus isolated from squirrel monkeys. J. Virol.23, 384–393 (1977).
Schochetman, G., Kortright, K., Schlom, J.: Mason-Pfizer monkey virus: Analysis and localisation of virion proteins and glycoproteins. J. Virol.16, 1208 to 1219 (1975).
Sciotti, C. G., Aikawa, M., Kohn, R. R.: The evaluation of AKR leukemia virus purity: The requirement for both velocity and isopycnic centrifugation methods. Anal. Biochem.73, 308–320 (1976).
Sen, A., Sherr, C. J., Todaro, G. J.: Phosphorylation of murine type C viral p 12 proteins regulates their extent of binding to the homologous viral RNA. Cell10, 489–496 (1977).
Sydow, G.: Eine einfache und schnelle Mikromethode zur Proteinbestimmung. Acta biol. med. germ.38, 1141–1144 (1979).
Voevodin, A. F., Wunderlich, V., Denner, J., Bierwolf, D., Lapin, B. A.: Common antigenic determinants on structural proteins p 10–12 of PMFV and MPMV type D retroviruses. Neoplasma, accepted for publication (1979).
Wunderlich, V., Zortter, S., Sydow, G.: RNA-dependent DNA polymerase activity in intracytoplasmic type-A particles of mouse mammary tumors. Acta biol. med. germ.35, 1741–1744 (1976).
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. Arnold Graffi on the occasion of his 70th birthday.
With 4 Figures
Rights and permissions
About this article
Cite this article
Uckert, W., Wunderlich, V., Bender, E. et al. The protein pattern of PMF virus, a type-D retrovirus from malignant permanent human cell lines. Archives of Virology 64, 155–166 (1980). https://doi.org/10.1007/BF01318019
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01318019