Summary
The genome of stomatitis papulosa virus (aparapoxvirus) was cleaved with the restriction endonucleasesHindIII andEcoRI, each giving rise to 6 fragments respectively. Double digestion with both enzymes resulted in 8 bands, two of which contained DNA fragments in double molar concentrations as revealed by reciprocal digests of isolated DNA fragments. The genome size, estimated by summation of the molecular weights of the fragments, is approximately 86×106 daltons, some 30×106 daltons smaller than vaccinia virus (anorthopoxvirus) DNA.
The cleavage sites ofHindIII andEcoRI endonucleases were mapped on the genome by analysis of reciprocal digests of isolated DNA fragments and by cross-hybridization experiments. This yielded two mapped segments which were then oriented relative to one another by cleavage of isolated partial digestion products.
The terminal restriction fragments show rapid renaturation after alkali denaturation and subsequent neutralization, indicating that stomatitis papulosa virus DNA contains terminal cross-links analogous to those found in vaccinia virus DNA.
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References
Berns, K. I., Silverman, C.: Natural occurrence of cross-linked vaccinia virus deoxyribonucleic acid. J. Virol.5, 299–304 (1970).
Denhart, D. T.: A membrane filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun.23, 641–646 (1966).
Doerfler, W., Kleinschmidt, A. K.: Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopy. J. mol. Biol.50, 579–593 (1970).
de Filippes, F. M.: Restriction enzyme digests of rapidly renaturing fragments of vaccinia virus DNA. J. Virol.17, 227–238 (1976).
Freifelder, D.: Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7, and the general problem of determination of M. J. mol. Biol.45, 567–577 (1970).
Geshelin, P., Berns, K. I.: Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA. J. mol. Biol.88, 785–796 (1974).
Joklik, W. K.: The purification of four strains of poxvirus. Virology18, 9–18 (1962).
Lang, D.: Molecular weights of coliphages and coliphage DNA. III. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus. J. mol. Biol.54, 557–565 (1970).
Maniatis, T., Jeffrey, A., Kleid, D. G.: Nucleotide sequences of the rightward operator of phage lambda. Proc. Nat. Acad. Sci. U.S.A.72, 1184–1188 (1975).
Mayr, A., Mahnel, H., Munz, E.: Systematisierung und Differenzierung der Pockenviren. Z. Bl. Vet. Med. B,19, 69–88 (1972).
Müller, H. K., Wittek, R., Schaffner, W., Schümperli, D., Menna, A., Wyler, R.: Comparison of five poxvirus genomes by analysis with restriction endonucleasesHindIII,BamI andEcoRI. J. gen. Virol.38, 135–147 (1978).
Munz, E.: Die Rinderpocken in der Sicht moderner virologischer Erkenntnisse. Berl. Münch. Tierärztl. Wschr.80, 201–220 (1967).
Murray, K., Murray, N. E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III ofHaemophilus influenza and restriction endonuclease I ofEscherichia coli. J. mol. Biol.98, 551–564 (1975).
Nagington, J., Newton, A. A., Horne, R. W.: The structure of orf virus. Virology23, 461–472 (1964).
Sarov, I., Becker, Y.: Studies on vaccinia virus DNA. Virology33, 369–375 (1967).
Schaffner, W., Gross, K., Telford, J., Birnstiel, M.: Molecular analysis of the histone gene cluster ofPsammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences. Cell8, 471–478 (1976).
Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. mol. Biol.98, 503–518 (1975).
Szybalski, W., Erikson, R. L., Gentry, G. A., Gafford, L. G., Randall, C. C.: Unusual properties of fowl poxvirus DNA. Virology19, 586–589 (1963).
Thomas, M., Davis, R. W.: Studies on the cleavage of bacteriophage lambda DNA withEcoRI restriction endonuclease. J. mol. Biol.91, 315–328 (1975).
Wittek, R., Menna, A., Schümperli, D., Stoffel, S., Müller, H. K., Wyler, R.:HindIII andSstI restriction sites mapped on rabbit poxvirus and vaccinia virus. J. Virol.23, 669–678 (1977).
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Menna, A., Wittek, R., Bachmann, P.A. et al. Physical characterization of a stomatitis papulosa virus genome: A cleavage map for the restriction endonucleasesHindIII andEcoRI. Archives of Virology 59, 145–156 (1979). https://doi.org/10.1007/BF01317904
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DOI: https://doi.org/10.1007/BF01317904