Summary
Antigenic content of a Raji cell surface extract (TS) was studied by sequential absorption of rabbit antiserum to this extract with different cells. Anti-TS was first allowed to react exhaustively with human blood cells followed by HeLa cells and human diploid fibroblasts. The residual reactivity was studied by stepwise absorption with an EBV-negative B lymphoblastoid cell line, BJAB, and then with B1–19, a clone derived from EBV transformed BJAB cells. These processes delineated three kinds of reactivities in anti-TS having specificities for antigens on B blasts, EBV-determined cell surface antigen(s) and antigens that are exclusive to Raji cells, respectively. These results are compatible with the heterogeneous protein content of TS and with the results of our earlierin vitro cell-mediated immunity studies using a similar preparation of Raji cell TS.
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References
Boyum, A.: Separation of Leukocytes from Blood and Bone Marrow. Scand. J. clin. Lab. Invest.21, Suppl. 97, 77–89 (1968).
Epstein, M. A., Achong, B. G., Barr, Y. M., Zajac, B., Henle, G., Henle, W.: Morphological and Virological Investigation on Cultured Burkitt Tumor Lymphoblasts (Strain Raji). J. Natl. Cancer Inst.37, 547–559 (1966).
Fresen, K. O., Merkt, B., Bornkamm, G. W., zur Hausen, H.: Heterogeneity of Epstein Barr Virus originating from P3HR-1 cells. I. Studies on EBNA Induction. Int. J. Cancer19, 317–323 (1977).
Green, S. S., Sell, K. W.: Mixed Leukocyte Stimulation of Normal Peripheral Leukocytes by Autologous Lymphoblastoid Cells. Science170, 989–990 (1970).
Katsuki, T., Hinuma, Y.: A Quantitative Analysis of the Susceptibility of Human Leukocytes to Transformation by Epstein-Barr Virus. Int. J. Cancer18, 7–13 (1976).
Klein, G.: The Epstein-Barr Virus. In:Kaplan, A. S. (ed.), The Herpesviruses, 521–555. New York — London: Academic Press 1973.
Klein, G., Giovanella, B. C., Lindahl, T., Fialkow, P. J., Singh, S., Stehlin, J.: Direct Evidence for the Presence of Epstein-Barr Virus DNA and Nuclear Antigen in Malignant Epithelial Cells from Patients with Poorly Differentiated Carcinoma of the Nasopharynx. Proc. Natl. Acad. Sci., U.S.A.71, 4737–4741 (1974).
Lo, E. H. M., Ng, M. H., Ng, W. S., Lui, S., Ho, H. C.: Study on the Surface Polypeptides Isolated from Human Lymphoblastoid Cells by Ionic Shock. Biochim. biophys. Acta444, 863–874 (1976).
Menezes, J., Leibold, W., Klein, G., Clements, G.: Establishment and Characterization of an Epstein-Barr Virus (EBV)-Negative Lymphoblastoid B Cell Line (BJAB) from an Exceptional EBV-genome Negative African Burkitt's Lymphoma. Biomedicine22, 276–284 (1975).
Miller, G., Lisco, H., Kohn, H. I., Stitt, D.: Establishment of Cell Lines from Normal Adult Human Blood Leukocyte by Exposure to Epstein-Barr Virus and Neutralization by Human Sera with Epstein-Barr Virus Antibody. Proc. Soc. exp. Biol. (N.Y.)137, 1459–1465 (1971).
Ng, W. S., Ng, M. H., Ho, H. C., Lamelin, J. P.:In vitro Cellular Immune Responses to PPD, Extracts from Raji Cells and Primary Nasopharyngeal Carcinoma (NPC) Biopsies observed in Leukocytes of NPC Patients and Controls. Brit. J. Cancer36, 713–722 (1977).
Nkrumah, F., Henle, W., Henle, G., Herberman, R., Perkins, V., Depur, R.: Burkitt's Lymphoma: Its Clinical Course in relation to Immunologic Reactivities to Epstein-Barr Virus and Tumor-related Antigens. J. Natl. Cancer Inst.57, 1051–1056 (1976).
Pope, J. H.: Transformationin vitro by Herpesviruses—A Review. In:De-Thé, G., Epstein, M. A., zur Hausen, H. (eds.), Oncogenesis and Herpesviruses I, 367–378. (IARC Scientific Publication No. 11, Pt. 1.) Lyon, France: IARC 1975.
Reedman, B. M., Klein, G.: Cellular Localization of an Epstein-Barr Virus (EBV)-associated Complement-fixing Antigen in Producer and Non-producer Lymphoblastoid Cell Lines. Int. J. Cancer11, 499–520 (1973).
Svedmyr, E., Jondal, M.: Cytotoxic Effector Cells Specific for B cell Lines Transformed by Epstein-Barr Virus are present in Patients with Infectious Mononucleosis. Proc. Natl. Acad. Sci. (Wash.)72, 1622–1626 (1975).
Ting, A., Mickey, M. R., Terasaki, P. I.: B Lymphocyte Alloantigens in Caucasians. J. exp. Med.143, 981–986 (1976).
Wolf, H., zur Hausen, H., Becker, V.: EB Viral Genomes in Epithelial Nasopharyngeal Carcinoma Cells. Nature (London) New Biol.244, 245–247 (1973).
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Ng, M.H., Ng, W.S. & Ho, H.C. Antigenic content of anin vitro cellular immunity reactive cell surface extract of Raji cells. Archives of Virology 58, 213–220 (1978). https://doi.org/10.1007/BF01317603
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DOI: https://doi.org/10.1007/BF01317603