Summary
The isolation of swinepox virus in primary pig kidney cell cultures is reported. The differentiation from vaccinia virus was possible with challenge infections of convalescent pigs and the use of the agar gel diffusion precipitation (AGDP) test and immuno-electroosmophoresis (IEOP). Using both immune precipitation tests reactions of identity were obtained between the heterologous antigens of swinepox and vaccinia viruses. A total of 829 pig sera from the field were tested for precipitating antibodies with the IEOP. Antibodies were detected in 65 (=7.8 per cent) of these serum samples.
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References
De Boer, G. F.: Equine rhinopneumonitis in Dutch horses. Proc. 1st Conf. on equine infectious diseases 101–111 (1966). (Grayson Foundation, Lexington, Ky.)
Cheville, N. F.: Immunofluorescent and morphologic studies on swinepox. Path. Vet.3, 556–564 (1966).
Cubie, H. A.: Serological studies in a student population prone to infection with human papilloma virus. J. Hyg. (Camb.)70, 677–690 (1972).
Datt, N. S.: Comparative studies of pig pox and vaccinia viruses. I. Host range pathogenicity. J. comp. Pathol.74, 62–68 (1964).
Datt, N. S., Orlans, E. S.: The immunological relationship of the vaccinia and pig pox viruses demonstrated by gel diffusion. Immunology1, 81–86 (1958).
Hilgers, J., Clydell Williams, W., Myers, B., Dmochowski, L.: Detection of antigens of the mouse mammary tumor (MTV) and murine leukemia virus (MuLV) in cells of cultures derived from mammary tumors of mice of several strains. Virology45, 470–483 (1971).
Garg, S. K., Meyer, R. C.: Adaptation of swinepox virus to an established cell line. Applied Microbiol.23, 180–182 (1972).
Kasza, L., Bohl, E. H., Jones, D. O.: Isolation and cultivation of swinepox virus in primary cultures of swine origin. Amer. J. vet. Res.21, 269–273 (1960).
Kasza, L., Griesemer, R. A.: Experimental Swine Pox. Amer. J. vet. Res.23, 443–450 (1962).
Mayr, A.: Experimentelle Untersuchungen über das Virus der originären Schweinepocken. Arch. ges. Virusforsch.9, 156–192 (1959).
Meyer, R. C., Conroy, J. D.: Experimental swinepox in gnotobiotic piglets. Res. vet. Sci.13, 334–338 (1972).
Pan, I. C., de Boer, C. J., Hess, W. R.: African swine fever: application of immuno-electroosmophoresis for the detection of antibody. Can. J. comp. Med.36, 309–316 (1972).
Shope, R. E.: Swinepox. Arch. ges. Virusforsch.1, 457–467 (1940).
Teppema, J. S., de Boer, G. F.: Ultrastructural aspects of experimental swinepox with special reference to inclusion bodies. Arch. Virology 49, 151–163 (1975).
Wallis, C., Melnick, J. L.: Enchanced detection of Australia antigen in serum hepatitis patients by discontinous counter-immunoelectrophoresis. Applied Microbiol.21, 867–869 (1971).
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de Boer, G.F. Swinepox. Virus isolation, experimental infections and the differentiation from vaccinia virus infections. Archives of Virology 49, 141–150 (1975). https://doi.org/10.1007/BF01317533
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DOI: https://doi.org/10.1007/BF01317533