Summary
Infectious bursal disease (IBD) virus was purified from the bursae of infected chickens. Two morphologically indistinguishable populations of virus particles were separated in sucrose gradients and possessed sedimentation coefficients of 395S and 460S. Both populations contained RNA and had identical polypeptide compositions. IBD virus banded at a density of 1.31 g/ml in CsCl and at 1.24 g/ml in sodium potassium tartrate.
IBD virus contained two RNA segments with mol. wts. of 2.4×106 and 2.2×106 as estimated by polyacrylamide-agarose gel electrophoresis, but sedimented in sucrose gradients at 15S. Virus RNA was resistant to 0.1 µg/ml ribonuclease treatment under conditions in which ribosomal RNA was completely hydrolysed, but was sensitive to 1.0 and 10 µg/ml treatments. These results suggest that the RNA consists of either double-stranded or highly ordered single-stranded molecules. IBD virus contained seven polypeptides with mol. wts. in the range 97,000 to 24,000. Two polypeptides were absent in empty particles of IBD virus.
IBD and infectious pancreatic necrosis (IPN) viruses were morphologically indistinguishable. IPN virus possessed a sedimentation coefficient of 440S and banded at a density of 1.32 g/ml in CsCl. In addition the electrophoretic mobilities of IBD and IPN virus RNAs were almost identical. Polyacrylamide slab gel electrophoresis showed that while the number and size of the polypeptides were different for each virus there were similarities in the overall pattern.
Similar content being viewed by others
References
Almeida, J. D., Morris, R.: Antigenically-related viruses associated with infectious bursal disease. J. gen. Virol.20, 369–375 (1973).
Cheville, N. F.: Studies on the pathogenesis of Gumboro disease in the bursa of Fabricius, spleen and thymus. Amer. J. Pathol.51, 527–551 (1967).
Cohen, J., Poinsard, A., Scherrer, R.: Physico-chemical and morphological features of infectious pancreatic necrosis virus. J. gen. Virol.21, 485–498 (1973).
Dobos, P.: Size and structure of the genome of infectious pancreatic necrosis virus. Nucleic Acid Res.3, 1903–1924 (1976).
Dobos, P., Hallett, R., Kells, D. T. C., Hill, B. J., Becht, H., Teninges, D.: Biophysical and biochemical studies of five animal viruses with bi-segmented ds RNA genomes. Abstracts of the Fourth International Congress of Virology held at the Hague, the Netherlands, p. 335, Centre for Agricultural Publishing and Documentation, Wagingen 1978.
Dobos, P., Hallett, R., Kells, D. T. C., Sorensen, O., Rowe, D.: Biophysical studies of infectious pancreatic necrosis virus (IPNV). J. Virol.22, 150–159 (1977).
Dobos, P., Rowe, D.: Peptide map comparison of infectious pancreatic necrosis virus-specific polypeptides. J. Virol.24, 805–820 (1977).
Harkness, J. W., Alexander, D. J., Pattison, M., Scott, A. C.: Infectious bursal disease agent morphology by negative stain electron microscopy. Arch. Virol.48, 63–73 (1975).
Hirai, K., Shimikura, S.: Structure of infectious bursal disease virus. J. Virol.14, 957–967 (1974).
Hoey, E. M., Martin, S. J.: A possible precursor containing RNA of a bovine enterovirus: the provirion 11. J. gen. Virol.24, 515–524 (1974).
Kelly, R. K., Loh, P. C.: Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus. J. Virol.10, 824–834 (1972).
Martin, S. J., Johnston, M., Clements, J. B.: Purification and characterization of bovine enteroviruses. J. gen. Virol.7, 103–113 (1970).
McFerran, J. B.: Bovine enteroviruses. Ann. New York Acad. Science101, 436–443 (1962).
McNulty, M. S., Allan, G. M., McFerran, J. B.: Cell culture studies with a cytopathic bovine rotavirus. Arch. Virol.54, 201–209 (1977).
McNulty, M. S., Gowans, E. J., Houston, M. J., Fraser, G.: Neuraminidase content of strains of Newcastle disease virus which differ in virulence. J. gen. Virol.27, 399–402 (1975).
Nick, H., Cursiefen, D., Becht, H.: Structural and growth characteristics of infectious bursal disease virus. J. Virol.18, 227–234 (1976).
Pattison, M., Alexander, D. J., Harkness, J. W.: Purification and preliminary characterization of a pathogenic strain of infectious bursal disease virus. Avian Pathol.4, 175–187 (1975).
Planterose, D. N., Birch, P. J., Pilch, D. J. F., Sharpe, T. J.: Antiviral activity of the double-stranded RNA and virus-like particles fromPenicillium stoloniferum. Nature (London)227, 504–505 (1970).
Todd, D., McNulty, M. S.: Characterization of pig rotavirus RNA. J. gen. Virol.33, 147–150 (1976).
Todd, D., McNulty, M. S.: Biochemical studies on a reovirus-like agent (rotavirus) from lambs. J. Virol.21, 1215–1218 (1977).
Underwood, B. O., Smale, C. J., Brown, F., Hill, B. J.: Relationship of a virus fromTellina tenuis to infectious pancreatic necrosis virus. J. gen. Virol.36, 93–109 (1977).
Author information
Authors and Affiliations
Additional information
With 5 Figures
Rights and permissions
About this article
Cite this article
Todd, D., McNulty, M.S. Biochemical studies with infectious bursal disease virus: Comparison of some of its properties with infectious pancreatic necrosis virus. Archives of Virology 60, 265–277 (1979). https://doi.org/10.1007/BF01317498
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317498