Summary
Electrophoretic mobility differences in polyacrylamide gels were detected between (35S)-methionine-labelled nucleoproteins (NPs) induced in monolayer cells by 15 human and 4 avian reference strains of influenza viruses. The (35S)-methionine-labelled tryptic peptides of nucleoproteins of these strains were also analyzed by peptide mapping technique. Based on several detectable hydrophilic peptides the NPs could be arranged in 7 clearly differentiable groups. After radioiodination of NPs from 4 human and 3 avian reference strains the tryptic peptide patterns showed one clear difference between human and avian strains.
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Geisler, B., Seidel, W., Herrmann, B. et al. Differences of nucleoproteins of human and avian influenza a virus strains shown by polyacrylamide gel electrophoresis and by the peptide mapping technique. Archives of Virology 90, 289–299 (1986). https://doi.org/10.1007/BF01317377
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DOI: https://doi.org/10.1007/BF01317377