Summary
Human neuroblastoma (IMR-32) cells were infected with herpes simplex virus type 2 (HSV-2) at a multiplicity of infection (MOI) of 2 plaque-forming units (PFU)/cell and were cultured at 40° C for 14 days. Then neither infectious virus particles nor virus capsids were detected in these cells whereas the presence of virus-specific antigens was observed by immunofluorescent antibody staining technique in 16.9±3.2 per cent of the infected cell population. When the cultivation temperature was shifted down from 40° C to 35° C, reactivation of virus growth occurred after lag periods of 2–9 days. These findings indicate that the IMR-32 cells can be latently infected with HSV-2 at 40° C and that virus growth may be inhibited at the level of synthesis of virus-specific macromolecules or at some step preceding nucleocapsid formation.
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Yura, Y., Terashima, K., Iga, H. et al. A latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line IMR-32. Archives of Virology 90, 249–260 (1986). https://doi.org/10.1007/BF01317374
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DOI: https://doi.org/10.1007/BF01317374