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MAV-2-O replicates at a reduced rate in cells from the osteopetrosis resistant G-B1 chicken

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Summary

The replication of the avian osteopetrosis virus MAV-2-O was compared in chick embryo fibroblasts from two strains of chicken. These were G-B1 which is relatively resistant to MAV-2-O and CB which is susceptible. The production of MAV-2-O was delayed in G-B1 cells (compared with CB cells). The same result was observed after infection with Rous sarcoma viruses of subgroups B, C, and D. In addition, the transforming viruses induced foci on G-B1 fibroblasts 24 to 48 hours later than on CB fibroblasts. In G-B1 cells there was also a delayed kinetics of intracellular viral RNA production. Integrated and linear unintegrated MAV-2-O DNA species were also present in lower amounts in G-B1 than in CB fibroblasts at 3 days postinfection.In vivo studies confirmed thein vitro situation. There was a marked difference in the amount of virus present in the osteoid bone matrix and the osteocytic lacunae of osteopetrotic bones from susceptible and G-B1 chickens. In contrast to the bone lesions from susceptible animals, budding virus particles were not detectable in lesions from G-B1 chickens. There was no difference in the amount of virus in osteopetrotic and non-osteopetrotic bone of susceptible chickens suggesting that virus replication alone is not sufficient for induction of osteopetrosis and that an additional specific virus-cell interaction is required. The relative resistance of strain G-B1 may therefore, be a consequence of a reduced frequency of this interaction. Its basis may be the lower amount of integrated, as well as unintegrated, viral DNA.

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References

  1. Biggs PM, Milne BS, Graf T, Bauer H (1973) Oncogenicity of non-transforming mutants of avian sarcoma viruses. J Gen Virol 18: 399–403

    Google Scholar 

  2. Böni-Schnetzler M, Böni J, Ferdinand F-J, Franklin RM (1985) Developmental and molecular aspects of nephroblastomas induced by avian myeloblastosis-associated virus 2-O. J Virol 55: 213–222

    Google Scholar 

  3. Böni J, Böni-Schnetzler M, Vainio E, Franklin RM (1986) Chicken strain G-B1 exhibits a relative resistance to avian osteopetrosis. Arch Virol 91: 21–36

    Google Scholar 

  4. Chirgwin JM, Przybyla AE, Macdonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299

    Google Scholar 

  5. Frank RM, Franklin RM (1982) Electron microscopy of avian osteopetrosis induced by retrovirus MAV-2-O. Calcif Tissue Int 34: 382–390

    Google Scholar 

  6. Fung Y-KT, Fadly AM, Crittenden LB, Kung H-J (1981) On the mechanism of retrovirus-induced avian lymphoid leukosis: deletion and integration of the proviruses. Proc Natl Acad Sci U.S.A. 78: 3418–3422

    Google Scholar 

  7. Huleihel M, Aboud M (1983) Inhibition of retrovirus DNA supercoiling in interferon-treated cells. J Virol 48: 120–126

    Google Scholar 

  8. Humphries EH, Danhof ML, Hlozanek I (1984) Characterization of endogenous viral loci in five lines of White Leghorn chickens. Virology 135: 125–138

    Google Scholar 

  9. Jeffreys AJ, Flavell RA (1977) A physical map of the DNA regions flanking the rabbit β-globin gene. Cell 12: 429–439

    Google Scholar 

  10. McMaster GK, Carmichael GC (1977) Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U.S.A. 74: 4835–4838

    Google Scholar 

  11. Morgan JJ, Smith RE (1983) Rapid induction of osteopetrosis by subgroup E recombinant viruses. Virology 129: 493–500

    Google Scholar 

  12. Moscovici C, Chi D, Gazzolo L, Moscovici MG (1976) A study of plaque formation with avian RNA tumor viruses. Virology 73: 181–189

    Google Scholar 

  13. Neel BG, Hayward WS, Robinson HL, Fang J, Astrin SM (1981) Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promotor insertion. Cell 23: 323–334

    Google Scholar 

  14. Neiman P, Beemon K, Luce JA (1981) Independent recombination between avian leukosis virus terminal sequences and host DNA in virus-induced proliferative disease. Proc Natl Acad Sci U.S.A. 78: 1896–1900

    Google Scholar 

  15. Nusse R, Varmus HE (1982) Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31: 99–109

    Google Scholar 

  16. Payne GS, Courtneidge SA, Crittenden LB, Fadly AM, Bishop JM, Varmus HE (1981) Analysis of avian leukosis virus DNA and RNA in bursal tumors: viral gene expression is not required for maintenance of the tumor state. Cell 23: 311–322

    Google Scholar 

  17. Perbal B, Cline JM, Hillyard RL, Baluda MA (1983) Organization of chicken DNA sequences homologous to the transforming gene of avian myeloblastosis virus. II. Isolation and characterization ofproto-amv DNA recombinant clones from a library of leukemic chicken DNA. J Virol 45: 925–940

    Google Scholar 

  18. Robinson HL, Miles BD (1985) Avian leukosis virus-induced osteopetrosis is associated with the persistent synthesis of viral DNA. Virology 141: 130–143

    Google Scholar 

  19. Schmidt EV, Keene JD, Linial M, Smith RE (1982) Association of 3′ terminal RNA sequences with avian leukosis viruses causing a high incidence of osteopetrosis. Virology 116: 163–180

    Google Scholar 

  20. Smith RE, Moscovici C (1969) The oncogenic effects of nontransforming viruses from avian myeloblastosis virus. Cancer Res 29: 1356–1366

    Google Scholar 

  21. Smith RE, Davids LJ, Neiman PE (1976) Comparison of an avian osteopetrosis virus with an avian lymphomatosis virus by RNA-DNA hybridization. J Virol 17: 160–167

    Google Scholar 

  22. Smith RE (1982) Avian osteopetrosis. In:Graf T, Jaenisch R (eds) Current topics in microbiology and immunology, vol 101. Springer, Berlin-Heidelberg-New York, pp 75–94

    Google Scholar 

  23. Smith RE, Morgan JH (1982) Identification of plaque isolates of an avian retrovirus causing rapid and slow onset osteopetrosis. Virology 119: 488–499

    Google Scholar 

  24. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  25. Vogt PK (1969) Focus assay of Rous sarcoma virus. In:Habel K, Salzman NP (eds) Fundamental techniques in virology. Academic Press, New York-London, pp 198–211

    Google Scholar 

  26. Weller SK, Joy AE, Temin HM (1980) Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus. J Virol 33: 494–506

    Google Scholar 

  27. Weller SK, Temin HM (1981) Cell killing by avian leukosis viruses. J Virol 39: 713–721

    Google Scholar 

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Authors and Affiliations

  1. Department of Structural Biology, Biocenter, University of Basel, Basel, Switzerland

    J. Böni, Marianne Böni-Schnetzler & R. M. Franklin

  2. Unité de Recherches INSERM U-157, Faculté de Chirurgie Dentaire, Université Louis Pasteur, Strasbourg, France

    R. M. Frank

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  1. J. Böni
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  2. Marianne Böni-Schnetzler
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  3. R. M. Frank
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  4. R. M. Franklin
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Böni, J., Böni-Schnetzler, M., Frank, R.M. et al. MAV-2-O replicates at a reduced rate in cells from the osteopetrosis resistant G-B1 chicken. Archives of Virology 91, 37–52 (1986). https://doi.org/10.1007/BF01316726

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  • DOI: https://doi.org/10.1007/BF01316726

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