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Host cell receptors for two strains of sindbis virus

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Summary

Experiments were performed to determine whether neuronal cells have different numbers of receptors for a neurovirulent and an avirulent strain of the same virus and whether neurotropic strains of different viruses share the same cellular receptors. Attempts were also made to characterize the receptors for the two strains of Sindbis virus on viable cells by studying their enzyme sensitivity.

The number of cellular receptors available for Sindbis virus attachment to several cell lines was determined by saturation studies using two virus strains differing in their pathogenicity for adult mice. Cultured neuronal cells had 1.3×106 receptors for a neurovirulent strain (SaAr86) and only 5×104 for the avirulent prototype strain (EgAr339) of Sindbis virus. A refractory Lepidopteran cell type possessed 1×105 surface receptors for the neurovirulent variant while the average number of receptors on five permissive cell types was 1.5×106. Cellular receptors for the two strains of Sindbis virus on rat glioma cells were found to be distinct.

The cellular receptors for EgAr339 on viable mammalian cells were sensitive to proteolytic cleavage, while those on living mosquito cells were insensitive to proteases, phospholipases and neuraminidase. The receptors for the neurovirulent variant on three mammalian cell types were less sensitive to enzymatic inactivation than those for the avirulent counterpart. After cleavage, the receptors for EgAr339 reappeared rapidly at 37° and 4° C, apparently in the absence of cellular synthesis.

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References

  1. Adams, M. H.: Bacteriophages, 29. New York: J. Wiley 1959.

    Google Scholar 

  2. Baulieu, E. E., Raynaud, J. P., Milgrom, E.: Measurement of steroid binding proteins. Acta endocrinol.64 (suppl. 147), 104–121 (1970).

    Google Scholar 

  3. Birdwell, C. R., Strauss, J. H.: Distribution of the receptor sites for Sindbis virus on the surface of chicken and BHK cells. J. Virol.14, 672–678 (1974).

    Google Scholar 

  4. Bose, H. R., Sagik, B. P.: The virus envelope in cell attachment. J. gen. Virol.9, 159–161 (1970).

    Google Scholar 

  5. Breakefield, X. O., Giller, E. L.: Neurotransmitter metabolism in cell culture. Biochem. Pharmacol.25, 2337–2342 (1976).

    Google Scholar 

  6. Casals, J.: Immunological techniques for animal viruses. In:Maramorosch, K., Koprowski, H. (eds.), Methods in Virology, Vol. 3, 113–198. New York: Academic Press 1967.

    Google Scholar 

  7. Chairez, R., Yoon, J.-W., Notkins, A. L.: Virus-induced diabetes mellitus. X. Attachment of encephalomyocarditis virus and permissiveness of cultured pancreatic β cells to infection. Virology8, 606–611 (1978).

    Google Scholar 

  8. Clark, H. F.: Rabies viruses increase in virulence when propagated in neuroblastoma cell culture. Science199, 1072–1075 (1978).

    Google Scholar 

  9. Clarke, D. H., Casals, J.: Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg.7, 561 to 573 (1958).

    Google Scholar 

  10. Dawson, G., Kemp, S. F., Stoolmiller, A. C., Dorfman, A.: Biosynthesis of glycosphingolipids by mouse neuroblastoma (NB 41 A), rat glia (RGC-6) and human glia (CHB-4) in cell culture. Biochem. biophys. Res. Comm.44, 687–694 (1971).

    Google Scholar 

  11. Dawson, G., Stoolmiller, A. C.: Comparison of the ganglioside composition of established neuroblastoma cell strains grownin vivo and in tissue culture. J. Neurochem.26, 225–227 (1976).

    Google Scholar 

  12. Dubois, M.-F.: Localization of the restrictive event of EMC virus replication in semi-permissive monkey and monkey-mouse hybrid cells. J. gen. Virol.37, 115 to 125 (1977).

    Google Scholar 

  13. Duffard, R. O., Fishman, P. H., Bradley, R. M., Lauter, C. J., Brady, R. O., Trams, E. G.: Ganglioside composition and biosynthesis in cultured cells derived from the CNS. J. Neurochem.28, 1161–1166 (1977).

    Google Scholar 

  14. Garvican, J. H., Brown, G. L.: A comparative analysis of the protein components of plasma membranes isolated from differentiated and undifferentiated mouse neuroblastoma cells in tissue culture. Europ. J. Biochem.76, 251–261 (1977).

    Google Scholar 

  15. Inglot, A. D., Chudzio, T., Albin, M.: Analysis of Sindbis virus and its soluble antigens in preparations concentrated by precipitation with polyethylene glycol or ammonium sulfate. Acta Virol.17, 416–425 (1973).

    Google Scholar 

  16. Johnson, R. T., McFarland, H. F., Levy, S. E.: Age-dependent resistance to viral encephalitis: studies of infections due to Sindbis virus. J. inf. Dis.125, 257 to 262 (1972).

    Google Scholar 

  17. Knudson, D. L., Buckley, S. M.: Invertebrate cell culture methods for the study of invertebrate-associated animal viruses. In:Maramorosch, K., Koprowski, H. (eds.), Methods in Virology, 323–391, New York: Academic Press 1977.

    Google Scholar 

  18. Knudson, D. L., Tinsley, T. W.: Replication of a nuclear polyhedrosis virus in a continuous cell culture ofSpodoptera frugiperda: purification, assay of infectivity, and growth chracteristics of the virus. J. Virol.14, 934–944 (1974).

    Google Scholar 

  19. Levinson, W., Oppermann, H., Rubenstein, P., Jackson, J.: Host range restriction of vesicular stomatitis virus on duck embryo cells. Virology85, 612–616 (1978).

    Google Scholar 

  20. Levitt, N. H., Crowell, R. L.: Comparative studies of the regeneration of HeLa cell receptors for poliovirus T 1 and Coxsackievirus B 3. J. Virol.1, 693–700 (1967).

    Google Scholar 

  21. Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Amer. Chem. Soc.56, 658–666 (1934).

    Google Scholar 

  22. Linser, P., Bruning, H., Armentrout, R. W.: Specific binding sites for a parvovirus, minute virus of mice, on cultured mouse cells. J. Virol.24, 211–221 (1977).

    Google Scholar 

  23. Lonberg-Holm, K., Korant, B. D.: Early interaction of rhinoviruses with host cells. J. Virol.9, 29–40 (1972).

    Google Scholar 

  24. McFarland, H. F., Johnson, R. T.: The role of the inflammatory response in viral infections. In:Notkins, A. L. (ed.), Viral Immunology and Immunopathology, 137–148. New York: Academic Press 1975.

    Google Scholar 

  25. McLaren, L. C., Holland, J. J., Syverton, J. T.: The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J. exp. Med.109, 475–485 (1959).

    Google Scholar 

  26. Mains, R. E., Patterson, P. H.: Primary cultures of dissociated sympathetic neurons. J. Cell Biol.59, 329–345 (1973).

    Google Scholar 

  27. Mitsuhashi, J., Maramorosch, K.: Cell cultures derived from larvae ofAedes albopictus (Skuse) andAedes aegypti (Singh). Contrib. Boyce Thompson Inst.22, 435 (1964).

    Google Scholar 

  28. Mooney, J. J., Dalrymple, J. M., Alving, C. R., Russel, P. K.: Interaction of Sindbis virus with liposomal model membranes. J. Virol.15, 225–231 (1975).

    Google Scholar 

  29. Neville, D. M.: The preparation of cell surface membrane enriched fractions. In:Maddy, A. H. (ed.), Biochemical Analysis of Membranes, 27–54. New York: J. Wiley 1976.

    Google Scholar 

  30. Pinteric, L., Taylor, J.: The lowered drop method for the preparation of specimens of partially purified virus lysates for quantitative electron micrographic analysis. Virology18, 359–371 (1962).

    Google Scholar 

  31. Postic, B.: Studies on the role of interferon in experimental infections of mice with Sindbis virus. Diss. Abstr.B28, 3375–3576 (1968).

    Google Scholar 

  32. Riggs, D. S.: The Mathematical Approach to Physiological Problems, 272–296. Baltimore: Williams and Wilkins 1963.

    Google Scholar 

  33. Rodbard, D.: Mathematics of hormone-receptor interactions. In:O'Malley, B. W., Means, A. R. (eds.), Receptors for Reproductive Hormones, 289–326, New York: Plenum Press 1973.

    Google Scholar 

  34. Scheffler, I. E., Buttin, G.: Conditionally lethal mutations in Chinese hamster cells. Isolation of a temperature-sensitive line and its investigation by cell cycle studies. J. Cell. Physiol.81, 199–216 (1973).

    Google Scholar 

  35. Smith, A. L., Tignor, G. H., Emmons, R. W., Woodie, J. D.: Isolation of field strains of rabies virus in CER and murine neuroblastoma cell cultures. Intervirology9, 359–361 (1978).

    Google Scholar 

  36. Smith, A. L., Tignor, G. H., Mifune, K., Motohashi, T.: Isolation and assay of rabies serogroup viruses in CER cells. Intervirology8, 92–99 (1977).

    Google Scholar 

  37. Sorensen, S. P. L.: Über die Messung und Bedeutung der Wasserstoffionenkonzentration bei biologischen Prozessen. Ergebn. Physiol.12, 393 (1912).

    Google Scholar 

  38. Stollar, V., Stollar, B. D., Koo, R., Harrap, K. A., Schlesinger, R. W.: Sialic acid contents of Sindbis virus from vertebrate and mosquito cells. Equivalence of biological and immunological properties. Virology69, 104–115 (1976).

    Google Scholar 

  39. Suitor, E. C.: Plaque formation by an arbovirus in a mosquito cell line. J. gen. Virol.5, 545–546 (1969).

    Google Scholar 

  40. Taylor, R. M., Hurlbut, H. S., Work, T. H., Kingston, J. R., Frothingham, T. E., Sindbis virus: a newly recognized arthropod-transmitted virus. Am. J. trop. Med. Hyg.4, 844–862 (1955).

    Google Scholar 

  41. Tignor, G. H., Shope, R. E., Bhatt, P. N., Percy, D. H.: Experimental infection of dogs and monkeys with two rabies serogroup viruses, Lagos bat and Makola (IbAn 27377): Clinical, serological, virologic and fluorescent-antibody studies. J. inf. Dis.128, 471–478 (1973).

    Google Scholar 

  42. Weinbren, M. P., Kokernot, R. H., Smithburn, K. C.: Strains of Sindbis-like virus isolated from culicine mosquitoes in the Union of South Africa. S. Afr. Med. J.30, 631–636 (1956).

    Google Scholar 

  43. Westphal, U.: Steroid-protein interactions. Monog. Endocrinol.4, 71–75 (1971).

    Google Scholar 

  44. Wivel, N. A., Yang, S. S.: Murine neuroblastoma clones with varying degrees of C-type virus expression. Int. J. Cancer18, 236–242 (1976).

    Google Scholar 

  45. Zajac, I., Crowell, R. L.: Effect of enzymes on the interaction of enteroviruses with living HeLa cells. J. Bacteriol.89, 574–582 (1965).

    Google Scholar 

  46. Zwaal, R. F. A., Roelofsen, B.: Applications of pure phospholipases in membrane studies. In:Maddy, A. H. (ed.), Biochemical Analysis of Membranes, 352 to 377. New York: J. Wiley 1976.

    Google Scholar 

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Authors and Affiliations

  1. Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut, USA

    Abigail L. Smith & G. H. Tignor

  2. Section of Comparative Medicine, Yale University School of Medicine, 375 Congree Avenue, 06510, New Haven, CT, USA

    Abigail L. Smith

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  1. Abigail L. Smith
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Smith, A.L., Tignor, G.H. Host cell receptors for two strains of sindbis virus. Archives of Virology 66, 11–26 (1980). https://doi.org/10.1007/BF01315041

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