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Proteins specified by African swine fever virus

II. Analysis of proteins in infected cells and antigenic properties

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Summary

Infection of MS cells with African swine fever virus (ASFV) produces inhibition of protein synthesis which is detectable from 4.5 hours after infection. At least 34 viral polypeptides have been indentified with molecular weights ranging between 9500 and 243,000 daltons. Three of these proteins show affinity for the cell nucleus and nine are in both the nuclear and cytoplasmic fractions. Ten early proteins were found, and most of the structural proteins were late proteins. Most of the proteins are synthesized within the first 8 hours after infection.

At least nine proteins induced antibodies in the natural infection. Six of these proteins are structural proteins. The antigenic determinants of VP172, VP162, VP146, VP73, VP34, and IP23.5 are in the primary structure of the proteins.

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References

  1. Andrews, Ch., Pereira, H. G.: Viruses of vertebrates, 3rd ed., 451. London: Baillière Tindall 1972.

    Google Scholar 

  2. Bjerrum, O. J., Lundahl, P.: Crossed immunoelectrophoresis of human erythrocyte membrane proteins. Immunoprecipitation patterns for fresh and stored samples of membranes extensively solubilized with non-ionic detergents. Biochim. biophys. Acta342, 69–80 (1974).

    Google Scholar 

  3. Breese, S. S., de Boer, C. J.: Electron microscope observations of African swine fever virus in tissue culture cells. Virology28, 420–428 (1966).

    Google Scholar 

  4. Breese, S. S., de Boer, C. J.: Effect of hydroxyurea on the development of African swine fever virus. Amer. J. Pathol.55, 69–77 (1969).

    Google Scholar 

  5. Coggins, L.: Growth and certain stability characteristics of African swine fever virus. Am. J. vet. Res.27, 1351–1358 (1966).

    Google Scholar 

  6. Dalsgaard, K., Overby, E., Sánchez-Botija, C.: Crossed immunoelectrophoresis was applied for the characterization of 5 specific antigens in African swine fever virus infected cells. Monospecific antisera were prepared against 3 individual antigens in the precipitation arcs. J. gen. Virol.36, 203–206 (1977).

    Google Scholar 

  7. de Boer, C. J., Pan, I. C., Hess, W. R.: Immunology African swine fever. J.A.V.M.A.160, 528–533 (1972).

    Google Scholar 

  8. Goorha, R., Granoff, A.: Macromolecular synthesis in cells infected by frog virus 3. I. Virus-specific protein synthesis and its regulation. Virology60, 237–250 (1974).

    Google Scholar 

  9. Kessler, S. W.: Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J. Immunol.115, 1617–1624 (1975).

    Google Scholar 

  10. Lee, L. D., Baden, H. P.: The use of denaturing conditions for gel diffusion of insoluble epidermal proteins. J. immunol. Methods18, 381–385 (1977).

    Google Scholar 

  11. Lee, L. D., Baden, H. P., Cheng, Ch. K.: Rocket immunoelectrophoresis in the presence of denaturing agents. J. immunol. Methods24, 155–162 (1978).

    Google Scholar 

  12. Moreno, M. A., Carrascosa, A. L., Ortín, J., Viñuela, E.: Inhibition of African swine fever (ASF) virus replication by phosphonoacetic acid. J. gen. Virol.93, 253–258 (1978).

    Google Scholar 

  13. Moura Nunes, J. F., Vigario, J. D., Terrinha, A. M.: Ultrastructural study of African swine fever virus replication in cultures of swine bone marrow cells. Arch. Virol.49, 59–66 (1975).

    Google Scholar 

  14. Norrild, B., Vestergaard, B. F.: Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel. J. Virol.22, 113–117 (1977).

    Google Scholar 

  15. Ortín, J., Viñuela, E.: Requirement of cell nucleus for African swine fever virus replication in vero cells. J. Virol.21, 902–905 (1977).

    Google Scholar 

  16. Pan, I. C., de Boer, C. J., Hess, W. R.: African swine fever: Application of immunoelectroosmophoresis for the detection of antibody. Canad. J. comp. Med.36, 309–316 (1971).

    Google Scholar 

  17. Pennington, T. H.: Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides. J. gen. Virol.25, 433–444 (1974).

    Google Scholar 

  18. Sánchez-Botija, C., McAuslan, B. R., Tabarés, E., Wilkinson, P., Ordás, A., Friedmann, A., Solana, A., Fereira, C., Ruiz-Gonzalvo, F., Dalsgaard, C., Marcotegui, M. A., Becker, Y., Schlomai, J.: Studies on African swine fever virus: purification and analysis of virions. Commission of the European Communities EUR 5626e (1977).

  19. Tabarés, E., Marcotegui, M. A., Fernández, M., Sánchez-Botija, C.: Proteins specified by African swine fever virus I. Analysis of the structural proteins and antigenic properties. Arch. Virol.66, 107–117 (1980).

    Google Scholar 

  20. Tabarés, E., Sánchez-Botija, C.: Synthesis of DNA in cells infected with African swine fever virus. Arch. Virol.61, 49–59 (1979).

    Google Scholar 

  21. Vigario, J. D., Relvas, M. E., Ferraz, F. P., Ribeiro, J. M., Pereira, C. G.: Identification and localization of genetic material of African swine fever virus by autoradiography. Virology33, 173–175 (1967).

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Virología Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain

    E. Tabarés, J. Martinez, F. Ruiz Gonzalvo & C. Sánchez-Botija

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  1. E. Tabarés
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  2. J. Martinez
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  3. F. Ruiz Gonzalvo
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  4. C. Sánchez-Botija
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Tabarés, E., Martinez, J., Gonzalvo, F.R. et al. Proteins specified by African swine fever virus. Archives of Virology 66, 119–132 (1980). https://doi.org/10.1007/BF01314980

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  • DOI: https://doi.org/10.1007/BF01314980

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