Summary
The hemagglutination (HA) and receptor destroying enzyme (RDE) activities of a newly isolated mouse enteric coronavirus (designated as DVIM) are described. DVIM agglutinates mouse or rat red blood cells (RBC) at 4° C. At 37° C the agglutination was rapidly reversed. The optimal pH for HA and for RDE activities using mouse red cells were shown to be 6.5 and 7.3 respectively. Hemagglutination by DVIM was not inhibited by pretreatment of RBCs withVibrio cholerae filtrate or by pretreatment with Influenza-A neuraminidase. Therefore, the DVIM receptors on RBCs differ from the receptors of Influenza-A, and the RDE activity of DVIM acts specifically on this receptor.
In addition, an analysis of the DVIM polypeptides showed that the virions contain five major, VP1 (M.W. 139,000), VP2 (68,000), VP3 (53,000), VP4 (38,000), VP5 (22,000) and two minor, VP1a (110,000), VP1b (100,000) polypeptides. VP1 and VP1b were digested by bromelain, suggesting that they constitute the surface glycoproteins.
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Bingham, R. W., Madge, M. H., Tyrrell, D. A.: Hemagglutination by avian infectious bronchitis virus—a coronavirus. J. gen. Virol.28, 381–390 (1975).
Bridger, J. C., Caul, E. O., Egglestone, S. I.: Replication of an enteric bovine coronavirus in intestinal organ cultures. Arch. Virol.57, 43–51 (1978).
Bucknall, R. A., Kalica, A. R., Chanock, R. M.: Intracellular development and mechanism of hemadsorption of human coronavirus, OC43. Proc. Soc. exp. Biol. Med.139, 811–817 (1972).
Garwes, D. J., Pocock, D. H.: The polypeptide structure of transmissible gastroenteritis virus. J. gen. Virol.29, 25–34 (1975).
Garwes, D. J., Pocock, D. H., Pike, B. V.: Isolation of subviral components from transmissible gastroenteritis virus. J. gen. Virol.32, 283–294 (1976).
Greig, A. S., Mitchell, D., Corner, A. H., Bannister, G. L., Meads, E. G., Julian, R. J.: A hemagglutinating virus producing encephalomyelitis in baby pigs. Canad. J. comp. Med.26, 49–56 (1962).
Hajer, I., Storz, J.: Structural polypeptides of the enteropathogenic bovine coronavirus strain LY-138. Arch. Virol.59, 47–57 (1979).
Hierholzer, J. C., Palmer, E. L., Whitfield, S. G., Kaye, H. S., Dowdle, W. R.: Protein composition of coronavirus OC43. Virology48, 516–527 (1972).
Hierholzer, J. C.: Purification and biophysical properties of human coronavirus 229E. Virology75, 155–165 (1976).
Hirst, G. K.: The relationships of the receptors of a new strain of virus to those of the mumpus-NDV-influenza group. J. exp. Med.91, 177–184 (1950).
Kapikian, A. Z., James, H. D., Jr., Kelly, S. J., King, L. M., Vaughn, A. L., Chanock, R. M.: Hemadsorption by coronavirus strain OC-43. Proc. Soc. exp. Biol. Med.139, 179–186 (1972).
Kaye, H. S., Dowdle, W. R.: Some characteristics of hemagglutination of certain strains of “IBV-like” virus. J. inf. Disease120, 576–581 (1969).
Kendal, A. P.: A comparison of “influenza C” with prototype myxoviruses; Receptor-destroying activity (neuraminidase) and structural polypeptides. Virology65, 87–99 (1975).
Macnaughton, M. R., Madge, M. H.: The polypeptide composition of avian infectious bronchitis virus particles. Arch. Virol.55, 47–54 (1977).
Macnaughton, M. R., Madge, M. H., Davies, H. A., Dourmashkin, R. R.: Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. J. Virol.24, 821–825 (1977).
Macnaughton, M. R.: The polypeptides of human and mouse coronaviruses. Arch. Virol.63, 75–80 (1980).
Maizel, J. V.: Acrylamide-gel electrophorograms by mechanical fractionation; Radioactive adenovirus protein. Science151, 988–990 (1966).
McIntosh, K.: Coronavirus; A comparative review. Curr. Top. Microbiol. Immunol.63, 85–129 (1974).
Pocock, D. H., Garwes, D. J.: The polypeptides of hemagglutinating encephalomyelitis virus and isolated subviral particles. J. gen. Virol.37, 487–499 (1977).
Pensaert, M. B., Callebaut, P. E.: Characteristics of coronavirus causing vomition and wasting in pigs. Arch. ges. Virusforsch.44, 35–50 (1974).
Sato, K., Maru, M., Wada, T.: Some characteristics of corona-like virus isolated from infant mice with diarrhea and inflamentory submaxillary gland of rats (in Japanese). Virus26, 97 (1976).
Shapiro, A. L., Vinuela, E., Maizel, J. V.: Molecular weight estimation of polypeptide chain by electrophoresis in SDS-polyacrylamide gels. Biochem. Biophys. Res. Commun.28, 815–820 (1967).
Shimizu, K., Shimizu, Y. K., Kohama, T., Ishida, N.: Isolation and characterization of two distinct types of HVJ (Sendai virus) spikes. Virology62, 90–101 (1974).
Sturman, L. S.: Characterization of a coronavirus. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology77, 637–649 (1977).
Sturman, L. S., Holmes, K. V.: Characterization of a coronavirus. 2. Glycoproteins of the viral envelope; Tryptic peptide analysis. Virology77, 650–660 (1977).
Tyrrell, D. A. J., Almeida, J. D., Berry, D. M., Cunningham, C. H., Hamre, D., Hofstad, M. S., Mallucci, L., McIntosh, K.: Coronaviruses. Nature200, 650 (1968).
Wege, H., Wege, Hanna, Nagashima, K., ter Meulen, V.: Structural polypeptides of the murine coronavirus JHM. J. gen. Virol.42, 37–47 (1979).
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Sugiyama, K., Amano, Y. Hemagglutination and structural polypeptides of a new coronavirus associated with diarrhea in infant mice. Archives of Virology 66, 95–105 (1980). https://doi.org/10.1007/BF01314978
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DOI: https://doi.org/10.1007/BF01314978