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The effect of arginine deprivation on DNA, thymidine kinase and DNA polymerase synthesis in simian virus 40-infected monkey kidney cells

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In arginine-deprived cells infected with simian virus 40 (SV40), both viral DNA and viral structural proteins were synthesized but infectious virus was not produced. The syntheses of cellular DNA, thymidine kinase and DNA polymerase were induced in virus-infected cells deprived of arginine but the maximum rate of synthesis of these enzymes occurred much later than that in infected cells incubated in the presence of arginine.

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References

  1. Cheng, Y.-C., Prusoff, W. H.: A new rapid procedure for measuring deoxycytidylate- and deoxythymidylate-kinase activities. Anal. Biochem.60, 545–550 (1974).

    Google Scholar 

  2. Green, M.: Oncogenic viruses. Ann. Rev. Biochem.39, 701–756 (1970).

    Google Scholar 

  3. Hartwell, L. H., Vogt, M., Dulbecco, R.: Induction of cellular DNA synthesis by polyoma virus. II. Induction in the rate of enzyme synthesis after infection with polyoma virus in mouse kidney cells. Virology27, 262–272 (1965).

    Google Scholar 

  4. Hirt, B.: Selective extraction of polyoma DNA from infected mouse cell cultures. J. mol. Biol.26, 365–369 (1967).

    Google Scholar 

  5. Kit, S., Leung, W. C., Trkula, D., Dubbs, D. R., Jorgensen, G.: Gel electrophoresis, isoelectric focusing, and location of thymidine kinase in normal and simian virus 40-infected monkey cells. Intervirology2, 137–151 (1973/74).

    Google Scholar 

  6. Kit, S., Melnick, J. L., Dubbs, D. R., Piekarski, L. J., de Torres, R. A.: Early enzyme synthesis in cell cultures infected with papova viruses, SV 40 and polyoma. Perspectives in Virology5, 63–86 (1967).

    Google Scholar 

  7. Kit, S., de Torres, R. A., Dubbs, D. R., Salvi, M. L.: Induction of cellular deoxyribonucleic acid synthesis by simian virus 40. J. Virol.1, 738–746 (1967).

    Google Scholar 

  8. McAuslan, B. R.: The induction and repression of thymidine kinase in the poxvirus-infected cell. Virology21, 383–389 (1963).

    Google Scholar 

  9. Tan, K. B., McAuslan, B. R.: Binding of DNA polymerase to poxvirus. J. Virol.9, 70–74 (1972).

    Google Scholar 

  10. Tan, K. B., Sokol, F.: Enhancement of uptake of simian virus 40 by nuclei of permissive cells. Proc. Soc. exp. Biol. Med.144, 803–808 (1973).

    Google Scholar 

  11. Tan, K. B., Sokol, F.: Replication of simian virus 40 in permissive cells: assembly of viral components. J. gen. Virol.25, 37–51 (1974).

    Google Scholar 

  12. Tegtmeyer, P., Ozer, H.: Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J. Virol.8, 516–524 (1971).

    Google Scholar 

  13. Winocour, E.: Some aspects of the interaction between polyoma virus and cell DNA. Adv. Virus Res.14, 153–200 (1969).

    Google Scholar 

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  1. The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania, USA

    K. B. Tan

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  1. K. B. Tan
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Tan, K.B. The effect of arginine deprivation on DNA, thymidine kinase and DNA polymerase synthesis in simian virus 40-infected monkey kidney cells. Archives of Virology 53, 133–138 (1977). https://doi.org/10.1007/BF01314854

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  • DOI: https://doi.org/10.1007/BF01314854

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