Summary
Simian syncitium-forming (“foamy”) virus type I (SFV1) was characterized biochemically. RNA was extracted from purified virus either with 0.1 per cent SDS or by the standard phenol-chloroform method. By both techniques a main component of 65–70S was found. Denaturation of the 65–70S RNA by heat resulted in a shift of the sedimentation coefficient mainly to a 30–35S component. Electrophoresis on a composite polyacrylamide gel demonstrated the existence of three minor RNA's: 8S, 5S and 4S respectively. PAGE-SDS analysis of disrupted purified virions enabled the separate migration of five viral proteins and the identification of two main proteins: a 30 kd polypeptide and a 70 kd polypeptide.
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Cavalieri, F., Rhodes-Feuillette, A., Benzair, A.B. et al. Biochemical characterization of simian foamy virus type I. Archives of Virology 68, 197–202 (1981). https://doi.org/10.1007/BF01314572
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DOI: https://doi.org/10.1007/BF01314572