Summary
Several types of cell strains and established cell lines of simian and human origin failed to demonstrate foci of altered cells following infection with the Mason-Pfizer monkey virus (M-PMV). However, most diploid cultures, after infection, lived longer and displayed the ability to grow in soft agar medium. The number of cell colonies developing in the soft agar was directly proportional to the amount of virus added to the culture. Two types of cell colonies were isolated from soft agar after infection of monkey foreskin cells with M-PMV. One had characteristic fibroblastic morphology, and the other showed an epithelioid cell phenotype. The ratio of fibroblastic colonies to epithelioid colonies was in excess of 20:1. The epithelioid cultures displayed a complete lack of topoinhibition, formed three dimensional cellular dome structures, and demonstrated significant karyotypic alterations. Fibroblastic sublines, on the other hand, did not show formation of domes but presented some lack of topoinhibition. The majority of cells in fibroblastic sublines also continued to show a normal rhesus chromosome complement. Although both epithelioid and fibroblastic transformed cell types produced intracellular M-PMV antigen and virus particles, the infectious virus titers were significantly different. The noninfectious virus preparations recovered from some of the fibroblastic sublines contained a high percentage of aberrant forms of M-PMV.
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Ahmed, M., Yeh, J., Holden, H.E. et al. Characterization of Mason-Pfizer virus induced cell transformationin vitro . Archives of Virology 55, 93–105 (1977). https://doi.org/10.1007/BF01314483
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DOI: https://doi.org/10.1007/BF01314483