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Biological and biophysical characteristics of mouse adenovirus, strain FL

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Summary

Mouse adenovirus, strain FL (MAV), is best propagated in primary rather than secondary cultures of mouse kidney cells. Virus release from the cells into the medium is fairly efficient; the average yield per cell is about 1000 TCID50. Morphological and other biophysical characteristics are those of a typical adenovirus: the icosahedral shell with a diameter of 74 nm, fiber projections of 29 nm length, its localization inside the infected kidney cells, a buoyant density in CsCl of 1.34 g/ml, inhibition of multiplication by inhibitors of DNA synthesis. The thermostability is higher than that of human adenoviruses, whereas, in contrast to these, MAV is inactivated to a great extent by trypsin. Complete or incomplete hemagglutinin or toxin-like activity were not detected.

The virus shows no cross-neutralization with human adenoviruses and a one-sided cross-reaction with another murine adenovirus strain K87. Soluble complement-fixing antigen of MAV exhibits a sedimentation rate of 12S identical to the hexon component of human adenoviruses; both antigens show a partial antigenic relationship in tests with appropriate antisera.

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Authors and Affiliations

  1. Institut für Hygiene und Mikrobiologie, Universität des Saarlandes, Homburg (Saar), Germany

    R. Wigand

  2. Robert-Koch-Institut des Bundesgesundheitsamtes, Berlin

    H. Gelderblom & M. Özel

Authors
  1. R. Wigand
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  2. H. Gelderblom
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  3. M. Özel
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With 9 Figures

Aided by a grant from the Bundesministerium für Jugend, Familie und Gesundheit, Federal Republic of Germany.

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Wigand, R., Gelderblom, H. & Özel, M. Biological and biophysical characteristics of mouse adenovirus, strain FL. Archives of Virology 54, 131–142 (1977). https://doi.org/10.1007/BF01314385

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  • DOI: https://doi.org/10.1007/BF01314385

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