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Improved method for the purification of Hog Cholera Virus grown in tissue culture

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Summary

A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved by rate zonal centrifugation in a 7–35 per cent sucrose gradient. This procedure permits a recovery of approximately 40 per cent of viral infectivity. A specific infectivity of about 2×107 PFU/µg of protein was achieved. An apparent density d=1.13 g/ml in sucrose and a Sw 20 value of about 150 was determined for purified HC virions.

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  1. Institut National de la Recherche Agronomique, Station de Virologie-Immunologie, Thiverval-Grignon, France

    H. Laude

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Laude, H. Improved method for the purification of Hog Cholera Virus grown in tissue culture. Archives of Virology 54, 41–51 (1977). https://doi.org/10.1007/BF01314377

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  • DOI: https://doi.org/10.1007/BF01314377

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