Abstract
The intestinal digestive and absorptive function of the excluded jejunum was evaluated 6 and 12 weeks after jejunoileal bypass in rats. Brush border and cytosol aminopeptidase activities as well as mucosal sucrase activity were measured in self-emptying excluded jejunal segments following bypass surgery. In addition, followingin vivo perfusion of equimolar amounts of amino acid (l-leucine) and dipeptide (l-leucyl-l-leucine) solutions through bypassed jejunal segments, the uptake ofl-leucine was determined. Mucosal weight, protein, and DNA content were reduced in the bypass segment reflecting jejunal mucosal cell hypoplasia. Hydrolytic activities for all enzymes (including the subcellular fractions of the aminopeptidase activities) and absorption rates from both the free amino acid and peptide solutions were reduced in bypassed jejunal segments. When expressed on the basis of mucosal DNA content, however, no changes were observed. This study indicates that the functional alterations in the excluded jejunum after bypass are due to the reduced mucosal cell content of this segment.
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References
Matthews DM: Intestinal absorption of peptides. Physiol Rev 55:537–608, 1975
Matthews DM, Adibi SA: Peptide absorption. Gastroenterology 71:151–161, 1976
Freeman HJ, Kim YS: Protein digestion and absorption. Annu Rev Med 29:99–116, 1978
Freeman HJ, Kim YS, Sleisenger MH: Protein digestion and absorption in man. Normal mechanisms and protein-energy malnutrition. Am J Med (in press)
Fogel MR, Ravitch MM, Adibi SA: Absorptive and digestive functions of the jejunum after jejunoileal bypass for treatment of human obesity. Gastroenterology 71:729–733, 1976
Gleeson MH, Cullen J, Dowling RH: Intestinal structure and function after small bowel bypass in the rat. Clin Sci 43:731–742, 1972
Gleeson MH, Dowling RH, Peters TJ: Biochemical changes in intestinal mucosa after experimental small bowel bypass in the rat. Clin Sci 43:743–757, 1972
Holzbach RT: Hepatic effects of jejunoileal bypass for morbid obesity. Am J Clin Nutr 30:43–52, 1977
Moxley RT, Pozefsky T, Lockwood DH: Protein nutrition and liver disease after jejunoileal bypass for morbid obesity. N Engl J Med 290:921–926, 1974
Heimburger SL, Steiger E, Logerfo P, et al: Reversal of severe fatty hepatic infiltration after intestinal bypass for morbid obesity by calorie-free amino acid infusion. Am J Surg 129:229–235, 1975
Lockwood DH, Amatruda JM, Moxley RT, et al: Effect of oral amino acid supplementation on the liver disease after jejunoileal bypass for morbid obesity. Am J Clin Nutr 30:58–62, 1977
McCarthy DM, Kim YS: Changes in sucrase, enterokinase, and peptide hydrolase after intestinal resection. J Clin Invest 52:942–951, 1973
Lambert R: Surgery of the Digestive System of the Rat. Springfield, Illinois, Charles C Thomas, 1965, pp 413–419
Garrido AB, Freeman HJ, Chung YC, et al: Amino acid and peptide absorption after proximal small intestinal resection in the rat. Gut 20:114–120, 1979
Kim YS, McCarthy DM, Lane W, et al: Alterations in the levels of peptide hydrolases and other enzymes in brush border and soluble fractions of rat small intestinal mucosa during starvation and refeeding. Biochim Biophys Acta 321:262–273, 1973
Heizer WD, Kerley RL, Isselbacher KJ: Intestinal peptide hydrolases: differences between brush border and cytoplasmic enzymes. Biochim Biophys Acta 264:450–461, 1972
Nicholson JA, Kim YS: A one stepl-amino acid oxidase assay for intestinal peptide hydrolase activity. Anal Biochem 63:110–117, 1975
Silk DBA, Nicholson JA, Kim YS: Relationships between mucosal hydrolysis and transport of two phenylalanine dipeptides. Gut 17:870–876, 1976
Dahlquist A: Assay of intestinal disaccharidases. Anal Biochem 22:99–107, 1968
Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275, 1951
Kissane JM, Robins E: The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem 233:184–188, 1958
Hinegardner RT: An improved fluorometric assay for DNA. Anal Biochem 39:197–201, 1971
Adibi SA, Fogel MR, Agrawal RM: Comparison of free amino acid and dipeptide absorption in the jejunum of sprue patients. Gastroenterology 67:586–591, 1974
McClelland RN, Deshazo CV, Heimbach DM, et al: Prevention of hepatic injury after jejunoileal bypass by supplemental jejunostomy feedings. Surg Forum 21:368–370, 1970
Tompkins RK, Waisman J, Watt CM-H, Corlin R, Keith R: Absence of mucosal atrophy in human small intestine after prolonged isolation. Gastroenterology 73:1046–1049, 1977
Solhaug SH: Morphometric studies of the small intestine following jejunoileal shunt operation. Scand J Gastroenterol 11:155–160, 1976
Kozloff L, Jackson MJ, Joseph WL: Changes in intestinal function following jejunoileal bypass in primates. J Surg Res 20:607–612, 1976
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Supported by U.S. Public Health Service grant AM-17938 and VA Medical Research Service.
Dr. Garrido was a recipient of a research grant from Fundacao de Amparo a Pesquisa do Estado (FAPE), Sao Paulo, Brazil.
Dr. Freeman was a recipient of a research fellowship award from the Medical Research Council of Canada, Ottawa, Ontario. Present address: U.B.C. Cancer Research Center, 601 West 10 Avenue, Vancouver, British Columbia, Canada.
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Garrido, A.B., Freeman, H.J. & Kim, Y.S. Amino acid and peptide absorption in bypassed jejunum following jejunoileal bypass in rats. Digest Dis Sci 26, 107–112 (1981). https://doi.org/10.1007/BF01312225
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DOI: https://doi.org/10.1007/BF01312225