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Biliary excretion of radioactivity after intravenous administration of [3H]25-hydroxyvitamin D3 in man

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Abstract

The biliary excretion of radioactivity after intravenous [3H]25-hydroxyvitamin D3 was studied in nine patients with T-tube bile drainage. The mean±SD 24-hr radioactivity excretion in T-tube bile expressed as a percentage of the administered dose was 6.7±2.9%; after correction for incomplete bile collection, the value obtained was 16.0±11.1%. Chloroform solubility of biliary radioactivity increased from 27.4±8.9% to 72.9 ±10.1% following incubation with β-glucuronidase. High-performance liquid chromatographic analysis of chloroform extracts of bile revealed that most of the eluted radioactivity was more polar than [3H]25-hydroxyvitamin D3. No free [3H]25-hydroxyvitamin D3 was demonstrated. Thus in man, most of the biliary radioactivity excreted following [3H]25-hydroxyvitamin D3 is in the form of water-soluble compounds, mainly glucuronides. However, our results suggest that glucuronides of metabolites other than 25-OHD3 are predominantly formed.

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Author notes

  1. Juliet E. Compston MD

    Present address: Department of Pathology, Welsh National School of Medicine, Heath Park, CF4 4XN, Cardiff, U.K.

Authors and Affiliations

  1. Gastrointestinal Research Unit, The Rayne Institute, St. Thomas' Hospital, SE1 7EH, London, U.K.

    Julia E. Ledger MSc, Graham J. Watson &  Juliet E. Compston MD

Authors
  1. Julia E. Ledger MSc
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  2. Graham J. Watson
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  3. Juliet E. Compston MD
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We thank the Special Trustees, St. Thomas' Hospital, for generous financial support.

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Ledger, J.E., Watson, G.J. & Compston, J.E. Biliary excretion of radioactivity after intravenous administration of [3H]25-hydroxyvitamin D3 in man. Digest Dis Sci 31, 361–368 (1986). https://doi.org/10.1007/BF01311670

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  • DOI: https://doi.org/10.1007/BF01311670

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