Summary
Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA clones covering the genome region from the 3′ end of the pelomer gene to the start of the integral membrane protein gene. The nucleotide sequence of this area was determined using clone pTG11 and a previously reported cDNA clone pTG22. Three open reading frames (ORFs) were identified encoding putative polypeptides of relative molecular masses (Mr) 6,600, 27,600, and 9,200. The sequence encoding the Mr 9,200 polypeptide was found to be present on the “unique” 5′ region of the 3.0 kb mRNA species whereas the other two ORFs mapped on the 3.9 kb mRNA species. Differences between the ORFs from this strain of TGEV and those from a previously reported avirulent strain of TGEV were compared.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bilofsky HS, Burks C, Fickett JW, Goad WB, Lewitter FI, Rindone WP, Swindell CD, Tung C-S (1986) The Genbank genetic sequence database. Nucleic Acids Res 13: 1–4
Bohl EH, Gupta RKP, Olquin MV, Saif LJ (1972) Antibody responses in serum, clostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect Immun 6: 289–301
Boursnell MEG, Brown TDK (1984) Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B. Gene 29: 87–92
Boursnell MEG, Binns MM, Brown TDK (1985) Sequencing of coronavirus IBV genomic RNA: Three open reading frames in the 5′ ‘unique’ region of mRNA D. J Gen Virol 66: 2253–2258
Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987) Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol 68: 57–77
Britton P, Lee LG, Murfitt D, Boronat A, Jones-Mortimer MC, Kornberg HL (1984) Location and direction of transcription of theptsH andptsI genes on theEscherichia coli K 12 genome. J Gen Microbiol 130: 861–868
Britton P, Garwes DJ, Millson GC, Page K, Bountiff L, Stewart F, Walmsley J (1986) Towards a genetically-engineered vaccine against porcine transmissible gastroenteritis virus. In: Magnien E (ed) Biomolecular engineering in the European Community. Martinus Nijhoff, Dordrecht, pp 301–313
Britton P, Garwes DJ, Page K, Walmsley (1987) Expression of porcine transmissible gastroenteritis virus genes inE. coli as β-galactosidase chimaeric proteins. In: Lai MMC, Stohlman SA (eds) Coronaviruses. Plenum Press, New York London [Advances in experimental medicine and biology, vol 218, pp 55–64]
Britton P, Carmenes RS, Page KW, Garwes DJ, Parra F (1988a) Sequence of the nucleoprotein from a virulent British field isolate of transmissible gastroenteritis virus and its expression inSaccharomyces cerevisiae. Mol Microbiol 2: 89–99
Britton P, Carmenes RS, Page KW, Garwes DJ (1988b) The integral membrane protein from a virulent isolate of transmissible gastroenteritis virus: molecular characterization, sequence and expression inEscherichia coli. Mol Microbiol 2: 497–505
Brown TDK, Boursnell MEG, Binns MM (1984) A leader sequence is present on mRNA A of avian infectious bronchitis virus. J Gen Virol 65: 1437–1442
Budzilowicz CJ, Wilczynski SP, Weiss SR (1985) Three intergenic regions of coronavirus mouse hepatitis virus strain A 59 genome RNA contain a common nucleotide sequence that is homologous to the 3′ end of the viral mRNA leader sequence. J Virol 53: 834–840
Budzilowicz CJ, Weiss SR (1987) In vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A 59. Virology 157: 509–515
Ebner D, Siddell S (1987) Identification of the coronavirus MHV-JHM mRNA 4 gene product using fusion protein antisera. In: Lai MMC, Stohlman SA (eds) Coronaviruses. Plenum Press, New York London [Advances in experimental medicine and biology, vol 218, pp 39–45]
Ebner D, Raabe T, Siddell SG (1988) Identification of the coronavirus MHV-JHM mRNA 4 product. J Gen Virol 69: 1041–1050
Garwes DJ, Pocock DH (1975) The polypeptide structure of transmissible gastroenteritis virus. J Gen Virol 29: 25–34
Hamm GH, Cameron GN (1986) The EMBL data library. Nucleic Acids Res 14: 5–10
Jacobs L, van der Zeijst BAM, Horzinek MC (1986) Characterization and translation of transmissible gastroenteritis virus mRNAs. J Virol 57: 1010–1015
Kanehisa MI (1982) Los Alamos sequence analysis package for nucleic acids and proteins. Nucleic Acids Res 10: 183–196
Kapke PA, Brian DA (1986) Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. Virology 151: 41–49
Kapke PA, Tung FYC, Brian DA, Woods RD, Wesley R (1987) Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein. In: Lai MMC, Stohlman SA (eds) Coronaviruses. Plenum Press, New York London [Advances in experimental medicine and biology, vol 218, pp 117–122]
Klenk HD, Rott R (1980) Cotranslational and posttranslational processing of viral glycoproteins. Curr Top Microbiol Immunol 90: 19–48
Kozak M (1983) Comparison of initiation of protein synthesis in prokaryotes, eukaryotes and organelles. Microbiol Rev 47: 1–45
Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292
Lai MMC, Baric RS, Brayton PR, Stohlman SA (1984) Characterization of leader RNA sequences on the virion and mRNAs of mouse hepatitis virus, a cytoplasmic RNA virus. Proc Natl Acad Sci USA 81: 3626–3630
Laude H, Rasschaert D, Huet JC (1987) Sequence and N-terminal processing of the transmembrane protein E 1 of the coronavirus transmissible gastroenteritis virus. J Gen Virol 68: 1687–1693
Leibowitz JL, Perlman S, Weinstock G, DeVries JR, Budzilowicz C, Weissmann JM, Weiss SR (1988) Detection of a murine coronavirus nonstructural protein encoded in a down stream open reading frame. Virology 164: 156–164
Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227: 1435–1441
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Murphy G, Kavanagh T (1988) Speeding-up the sequencing of double-stranded DNA. Nucleic Acids Res 16: 5198
Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85: 2444–2448
Rasschaert D, Laude H (1987) The predicted structure of the peplomer protein E 2 of the porcine coronavirus transmissible gastroenteritis gastroenteritis virus. J Gen Virol 68: 1883–1890
Rasschaert D, Delmas B, Charley B, Grossclaude J, Gelfi J, Laude H (1987a) Surface glycoproteins of transmissible gastroenteritis virus: functions and gene sequence. In: Lai MMC, Stohlman SA (eds) Coronaviruses. Plenum Press, New York London [Advances in experimental medicine and biology, vol 218, pp 109–116]
Rasschaert D, Gelfi J, Laude H (1987b) Enteric coronavirus TGEV: partial sequence of the genomic RNA, its organisation and expression. Biochemie 69: 591–600
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Shieh C-K, Soe LH, Makino S, Chang M-F, Stohlman SA, Lai MMC (1987) The 5′-end sequence of the murine coronavirus genome: implications for multiple fusion sites in leader-primed transcription. Virology 156: 321–330
Skinner MA, Siddell SG (1985) Coding sequence of coronavirus MHV-JHM mRNA. J Gen Virol 66: 593–596
Skinner MA, Ebner D, Siddell SG (1985) Coronavirus MHV-JHM mRNA 5 has a sequence arrangement which potentially allows translation of a second down stream open reading frame. J Gen Virol 66: 581–592
Smith AR, Boursnell MEG, Binns MM, Brown TDK, Inglis SC (1987) Identification of a new gene product encoded by mRNA D of infectious bronchitis virus. In: Lai MMC, Stohlman SA (eds) Coronaviruses. Plenum Press, New York London [Advances in experimental medicine and biology, vol 218, pp 47–54]
Spaan WJM, Delius H, Skinner M, Armstrong J, Rottier P, Smeekens S, van der Zeijst BAM, Siddell SG (1983) Coronavirus mRNA synthesis involves fusion of non-contiguous sequences. EMBO J 2: 1839–1844
von Heijne G (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14: 4683–4690
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Britton, P., Otin, C.L., Alonso, J.M.M. et al. Sequence of the coding regions from the 3.0 kb and 3.9 kb mRNA. Archives of Virology 105, 165–178 (1989). https://doi.org/10.1007/BF01311354
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311354