Summary
A protein of 34kDa (Nx) was induced in vitro by the infection of fish cell cultures with the rhabdovirus agent of viral haemorrhagic septicaemia (VHS) of the trout. This protein only appeared as a major component in concentrated or intracellular labeled VHS virus but not in purified VHS or in the related infectious haematopoietic necrosis virus. That Nx protein is antigenically related to the nucleoprotein of purified virus was shown by its reaction with four antinucleoprotein monoclonal antibodies (at least 3 of them reacting non competitivly against different epitopes) and by immunoprecipitation with polyvalent international reference sera. The Nx protein was shown to be specifically associated with free non-infective particles isolated by ultracentrifugation which were confirmed to be nucleocapsids by electron microscopy.
This is a preview of subscription content, log in via an institution to check access.
Access this article
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Similar content being viewed by others
References
Basurco B, Coll JM (1989) Spanish isolates and reference strains of viral haemorrhagic septicaemia virus show similar protein size patterns. Bull Eur Assoc Fish Pathol 9: 92–95
Bernard J, Lecocq-Xhonneux F, Rossius M, Thiry ME, De Kinkelin P (1990) Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. J Gen Virol 71: 1669–1674
Bunschoten H, Klapmuts RJ, Claassen IJTM, Reyneveld SD, Osterhaus ADME, Uytdehaag FGCM (1989) Rabies virus-specific human T cells clones provide help for an in vitro antibody response against neutralizing antibody-inducing determinants of the viral glycoprotein. J Gen Virol 70: 1513–1521
Cohen J, Lenoir G (1974) Ultrastructure et morphologie de quatre rhabdovirus de poissons. Ann Rech Vet 5: 443–455
Coll JM (1987) Injection of physiological saline facilitates recovery of ascitic fluids for monoclonal antibody production. J Immunol Methods 104: 219–222
Coll JM (1988) Immunochemical recognition of the binding of C-reactive protein to solid-phase phosphorylethanolamine. Rev Esp Fisiol 44: 169–178
Coll JM (1989) Addition of reducing agents to the peroxidase-o-phenylenediamine buffer reduces background of enzymeimmunoassays. Rev Esp Fisiol 45: 41–46
Deuter A, Enzmann PJ (1986) Comparative biochemical and serological studies on two fish-pathogenic rhabdoviruses (VHS-V and SVC-V). J Vet Med 33: 36–46
De Kinkelin P (1972) Le virus d'Egtved. II. Purification. Ann Rech Vet 3: 199–208
De Kineklin P, Berre MB, Bernard J (1980) Viral haemorrhagic septicaemia of rainbow trout: selection of a thermoresistant virus variant and comparison of polypeptide synthesis with the wild-type virus strain. J Virol 36: 652–658
Dietzschold B, Lafon M, Wang H, Otvos L, Celis E, Wunner WH, Koprowski H (1987) Localization and immunological characterization of antigenic domains of the rabies virus internal N and NS proteins. Virus Res 8: 103–125
Ertl HCJ, Dietzschold B, Gore M, Otvos L, Larson JK, Wunner WH, Koprowski H (1989) Induction of rabies virus-specific T-helper cells by synthetic peptides that carry dominant T-helper cell epitopes of the viral ribonucleoprotein. J Virol 63: 2885–2892
Gilmore RD, Leong JC (1988) The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virology 167: 644–648
Hsu YL, Engelking HM, Leong JC (1985) Analysis of the quantity and synthesis of the virion proteins of infectious hematopoietic necrosis virus. Fish Pathol 20: 331–338
Iturralde M, Coll JM (1984) High stability hybrids producing monoclonal antibodies against human C-reactive protein. Rev Esp Fisiol 40: 279–288
Jimenez de la Fuente J, Marcotegui MA, San Juan ML, Basaurco B (1988) Diagnosis of viral diseases in salmonid farms in Spain. Bull Eur Assoc Fish Pathol 271: 3–4
Lenoir G, De Kinkelin P (1975) Fish rhabdoviruses comparative study of protein structure. J Virol 16: 259–262
Lorenzen N, Olesen NJ, Jorgensen PEV (1988) Production and characterization of monoclonal antibodies to four Egtved virus structural proteins. Dis Aquat Organ 4: 35–42
Mc Allister PE, Wagner RR (1975) Structural proteins of two salmonid rhabdoviruses. J Virol 15: 733–738
Martinez J, Coll JM (1988) Selection and clinical performance of monoclonal anti C-reactive proteins in ELISA quantitative assay. Clin Chim Acta 176: 123–132
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17: 208–212
Rueda AZ, Coll JM (1988) Cloning of myelomas and hybridomas in fibrin clots. J Immunol Methods 114: 213–217
Wagner RR, Schnaitman TC, Snyder RM (1969) Structural proteins of vesicular stomatitis viruses. J Virol 3: 395–399
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Basurco, B., Sanz, F., Marcotegui, M.A. et al. The free nucleocapsids of the viral haemorrhagic septicaemia virus contain two antigenically related nucleoproteins. Archives of Virology 119, 153–163 (1991). https://doi.org/10.1007/BF01310666
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310666