Summary
Infection of BHK cells by SFV increases the rate of uptake of [3H]MeGlc and of [3H]dGlc at ∼2 hours p.i. Infection by HSV increases the uptake of [3H]MeGlc and [3H]dGlc at ∼10 hours p.i.; the increased uptake is prevented by acyclovir. It is concluded that an increased sugar uptake by infected cells reflects an increased rate of transport across the plasma membrane and is the result of cellular changes caused by virus infection.
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Antoniades, H. N., Owen, A. J.: Growth factors and regulation of cell growth. Ann. Rev. Med.33, 445–463 (1982).
Carrasco, L., Lacal, J. C.: Permeabilization of cells during animal virus infection. Pharmacol. Therap.23, 109–145 (1983).
Carrasco, L., Smith, A. E.: Molecular biology of animal virus infection. Pharmacol. Therap.9, 311–355 (1980).
Dekker, S., Lipmann, F.: Transport of D-glucose by membrane vesicles from normal and avian sarcoma virus-transformed chicken embryo fibroblasts. Proc. Natl. Acad. Sci. U.S.A.78, 5358–5361 (1981).
Eisenthal, R., Cornish-Bowden, A.: The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem. J.139, 715–720 (1974).
Foster, K. A., Gill, K., Micklem, K. J., Pasternak, C. A.: Survey of virally mediated permeability changes. Biochem. J.190, 639–646 (1980).
Foster, K. A., Micklem, K. J., Agnarsdottir, G., Lancashire, C. L., Bogomolova, N. N., Boriskin, Y. S., Pasternak, C. A.: Myxoviruses do not induce non-specific alterations in membrane permeability early on in infection. Arch. Virol.77, 139–153 (1983).
Gray, M. A., Micklem, K. J., Brown, F., Pasternak, C. A.: Effect of vesicular stomatitis virus and Semliki Forest virus on uptake of nutrients and intracellular cation concentration. J. gen. Virol.64, 1449–1456 (1983).
Gray, M. A., Micklem, K. J., Pasternak, C. A.: Protein synthesis in cells infected with Semliki forest virus is not controlled by intracellular cation changes. Eur. J. Biochem.135, 299–302 (1983).
Gray, M. A., Austin, S. A., Clemens, M. J., Rodrigues, L., Pasternak, C. A.: Protein synthesis in Semliki Forest virus infected cells is not controlled by permeability changes. J. gen. Virol.64, 2631–2640 (1983).
Hatanaka, M., Hanafusa, H.: Analysis of a functional change on membrane in the process of cell transformation by Rous sarcoma virus: alteration in the characteristics of sugar transport. Virology41, 647–652 (1970).
Hatanaka, M., Huebner, R. J., Gilder, R. B.: Sugar uptake in murine sarcoma virus transformed mouse cells. J. Nat. Cancer Inst.43, 1091–1096 (1969).
Isselbacher, K. J.: Increased uptake of amino acids and 2-deoxy-D-glucose by virus-transformed cells in culture. Proc. Natl. Acad. Sci. U.S.A.69, 585–589 (1972).
Kletzien, R. F., Perdue, J. F.: Sugar transport in chick embryo fibroblasts. I. A functional change in the plasma membrane associated with the rate of cell growth. J. Biol. Chem.249, 3375–3382 (1974).
Kletzien, R. F., Perdue, J. F.: Regulation of sugar transport in chick embryo fibroblasts and in fibroblasts transformed by a temperature-sensitive mutant of the Rous sarcoma virus. J. Cell. Physiol.89, 723–728 (1976).
Knox, P., Pasternak, C. A.: The cell surface and growthin vitro In:Jamieson, G. A., Robinson, D. M. (eds.), Mammalian Cell Membranes, Vol. 5, 196–219. London: Butterworth 1977.
Landini, M. P.: Early enhanced glucose uptake in human cytomegalovirus-infected cells. J. gen. Virol.65, 1229–1232 (1984).
Martin, G. S., Veruta, S., Weber, M., Rubin, H.: Temperature-dependent alterations in sugar transport in cells infected by a ts mutant of RSV. Proc. Natl. Acad. Sci. U.S.A.68, 2739–2741 (1971).
Nairn, R. C.: Fluorescent Protein Tracing, 4th ed. Edinburgh-London-New York: Churchill Livingstone 1976.
Pasternak, C. A.: Normal and transformed cellsin vitro. In:Jamieson, G. A., Robinson, D. M. (eds.), Mammalian Cell Membranes, Vol. 5, 89–114. London: Butterworth 1977.
Peluso, R. W., Lamb, R. A., Choppin, P. W.: Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus of deprived of glucose. Proc. Natl. Acad. Sci. U.S.A.75, 6120–6124 (1978).
Plagemann, P. G. W.: 2DG transport by uninfected murine sarcoma virus-transformed and murine leukaemia virus-infected mouse cells. J. Cell. Physiol.82, 421–434 (1973).
Romano, A. H.: Is glucose transport enhanced in virus transformed mammalian cells? A dissenting view. J. Cell. Physiol.89, 737–744 (1976).
St. Jeor, S. C., Albrecht, T., Funk., F. D. Rapp, F.: Stimulation of cellular DNA synthesis by human cytomegalovirus. J. Virol.13, 353–362 (1974).
Schaeffer, H. J., Beauchamp, L., de Miranda, P., Elion, G. B., Collins, P., Bauer, D. J.: 9-(2-hydroxyethoxymethyl)guanine activity against viruses of the herpes group. Nature272, 583–585 (1978).
Schultz, S. G., Curran, P. F.: Coupled transport of sodium and organic solutes. Physiol. Revs.50, 637–718 (1970).
Stinski, M. F.: Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. Virology23, 751–767 (1977).
Weber, M. J., Evans, P. K., Johnson, M. A., McNair, T. F., Nakamura, D., Salter, D. W.: Transport of potassium, amino acids, and glucose by cells transformed by Rous sarcoma virus. Fed. Proc.43, 107–112 (1984).
White, M. K., Bramwell, M. E., Harris, H.: Hexose transport in hybrids between malignant and non-malignant cells. Nature294, 232–235 (1981).
White, M. K., Bramwell, M. E., Harris, H.: Kinetic parameters of hexose transport in hybrids between malignant and non-malignant cells. J. Cell Sci.62, 49–80 (1983).
Wohlheuter, R. M., Plagemann, P. G. W.: The role of transport and phosphorylation in nutrient uptake in cultured animals cells. Int. Rev. Cytol.64, 171–240 (1980).
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Gray, M.A., James, M.H., Booth, J.C. et al. Increased sugar transport in BHK cells infected with semliki forest virus or with herpes simplex virus. Archives of Virology 87, 37–48 (1986). https://doi.org/10.1007/BF01310541
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DOI: https://doi.org/10.1007/BF01310541